MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 12 Aug 2025 at 22:47:09 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 41–50 (out of 313)

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+41

Accession Score Description
1 Q88DV1_PSEPK 291 ATP-dependent zinc metalloprotease FtsH OS=Pseudomonas putida (strain KT2440) GN=ftsH PE=3 SV=1

+42

Accession Score Description
1 ATPF_PSEPK 287 ATP synthase subunit b OS=Pseudomonas putida (strain KT2440) GN=atpF PE=3 SV=1

+43

Accession Score Description
1 RS9_PSEPK 277 30S ribosomal protein S9 OS=Pseudomonas putida (strain KT2440) GN=rpsI PE=3 SV=1

+44

Accession Score Description
1 RL18_PSEPK 277 50S ribosomal protein L18 OS=Pseudomonas putida (strain KT2440) GN=rplR PE=3 SV=1

+45

Accession Score Description
1 Q88KF1_PSEPK 276 Aconitate hydratase B OS=Pseudomonas putida (strain KT2440) GN=acnB PE=3 SV=1

+46

Accession Score Description
1 Q88Q24_PSEPK 275 ABC transporter, ATP-binding protein OS=Pseudomonas putida (strain KT2440) GN=PP_0674 PE=3 SV=1

+47

Accession Score Description
1 RL14_PSEPK 274 50S ribosomal protein L14 OS=Pseudomonas putida (strain KT2440) GN=rplN PE=3 SV=1

-48

Accession Score Description
1 Q88MH2_PSEPK 260 Outer membrane protein assembly factor BamA OS=Pseudomonas putida (strain KT2440) GN=bamA PE=3 SV=1
Score Mass Matches Sequences emPAI
48.1 Q88MH2_PSEPK 260 86575 14 (6) 13 (6) 0.17
Outer membrane protein assembly factor BamA OS=Pseudomonas putida (strain KT2440) GN=bamA PE=3 SV=1

-14 peptide matches (14 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
309   303.1460 604.2774 604.2704 11.7 0 0 5.9 +10Score > 23 indicates identity
Score > 20 indicates homology		 U K.QSGLAE.G + Deamidated (NQ)
329   311.6704 621.3262 621.3234 4.52 0 12 0.28 +5Score > 19 indicates identity U R.AYVNR.I
441   344.1936 686.3726 686.3711 2.22 0 14 0.55 +4Score > 24 indicates identity U R.VNGLQR.V + Deamidated (NQ)
467   350.7220 699.4294 699.4391 -13.8 1 2 4.8 +8Score > 22 indicates identity U R.NRVALK.I
468   700.4371 699.4298 699.4391 -13.3 1 13 0.43 +5Score > 22 indicates identity U R.NRVALK.I
565   745.4592 744.4519 744.4494 3.41 0 15 0.22 +2Score > 20 indicates identity U K.VSIGLTR.S
1457   572.3004 1142.5862 1142.5760 8.93 0 15 0.27 +1Score > 21 indicates identity U K.TTNWLSFFK.N
1698   626.8296 1251.6446 1251.6347 7.97 0 0 2.6 +2Score > 20 indicates identity
Score > 17 indicates homology		 U R.LTYGLSVQQDK.I + Deamidated (NQ)
1802   656.3427 1310.6708 1310.6619 6.82 0 67 1.3e-006 +1Score > 20 indicates identity U K.TGFFQDIQLSR.D
2071   722.4244 1442.8342 1442.8246 6.72 0 62 1.4e-006 +1Score > 16 indicates identity U K.SLLLVQPGQVFSR.K
2488   546.9703 1637.8891 1637.8777 6.94 0 51 2.8e-005 +1Score > 18 indicates identity U R.GQLFKPITSDYTLR.L
3315   1044.5276 2087.0406 2087.0284 5.88 0 108 8.5e-011 +1Score > 20 indicates identity U R.VSAGSVFGALPLNVGDQADDR.R
3590   746.3792 2236.1158 2236.1012 6.52 1 37 0.00095 +1Score > 19 indicates identity U R.GHSQSLTLESTVPGSDLSFFK.L
4158   929.1050 2784.2932 2784.2384 19.7 0 27 0.005 +1Score > 17 indicates identity U R.YNFGFVDPYFTADGVSLGYNAFYR.S + 2 Deamidated (NQ)

1 subset or intersection (1 subset protein in total)

Score Mass Subset of
Q88HI4_PSEPK 199 86574 48.1
Outer membrane protein assembly factor BamA OS=Pseudomonas putida (strain KT2440) GN=bamA PE=3 SV=1

+49

Accession Score Description
1 Q88PJ4_PSEPK 259 Outer membrane protein assembly factor BamB OS=Pseudomonas putida (strain KT2440) GN=bamB PE=3 SV=1

+50

Accession Score Description
1 Q88FM2_PSEPK 258 Acetyl-CoA carboxylase, biotin carboxylase, putative OS=Pseudomonas putida (strain KT2440) GN=PP_4067 PE=4 SV=1
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