MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita1 |
| MS data file | : | PRT1270_T-BRSC_1_20250714114344.mgf |
| Database | : | P-putida 20180924 (5,556 sequences; 1,892,173 residues) |
| Timestamp | : | 12 Aug 2025 at 22:47:09 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,938 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle () in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
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41| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88DV1_PSEPK | 291 | ATP-dependent zinc metalloprotease FtsH OS=Pseudomonas putida (strain KT2440) GN=ftsH PE=3 SV=1 |
42| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ATPF_PSEPK | 287 | ATP synthase subunit b OS=Pseudomonas putida (strain KT2440) GN=atpF PE=3 SV=1 |
43| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RS9_PSEPK | 277 | 30S ribosomal protein S9 OS=Pseudomonas putida (strain KT2440) GN=rpsI PE=3 SV=1 |
44| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL18_PSEPK | 277 | 50S ribosomal protein L18 OS=Pseudomonas putida (strain KT2440) GN=rplR PE=3 SV=1 |
45| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88KF1_PSEPK | 276 | Aconitate hydratase B OS=Pseudomonas putida (strain KT2440) GN=acnB PE=3 SV=1 |
46| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88Q24_PSEPK | 275 | ABC transporter, ATP-binding protein OS=Pseudomonas putida (strain KT2440) GN=PP_0674 PE=3 SV=1 |
47| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL14_PSEPK | 274 | 50S ribosomal protein L14 OS=Pseudomonas putida (strain KT2440) GN=rplN PE=3 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 47.1 | RL14_PSEPK |
274 | 13458 | 18 (15) | 10 (8) | 5.04 | |
| 50S ribosomal protein L14 OS=Pseudomonas putida (strain KT2440) GN=rplN PE=3 SV=1 | |||||||
18 peptide matches (13 non-duplicate, 5 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
| 276 |
585.3362 | 584.3289 | 584.3282 | 1.26 | 1 | 18 | 0.065 | 2Score > 19 indicates identity |
U | K.EAIPR.G | |
| 358 |
321.2032 | 640.3918 | 640.4020 | -15.9 | 1 | 4 | 0.59 | 1Score > 14 indicates identity |
U | E.AIPRGK.V | |
| 535 |
366.2067 | 730.3988 | 730.3973 | 2.07 | 0 | 36 | 0.0035 | 1Score > 24 indicates identity |
U | R.ADGSIIR.F | |
| 654 |
789.4647 | 788.4574 | 788.4545 | 3.73 | 0 | 19 | 0.032 | 1Score > 17 indicates identity |
U | R.IFGPVTR.E | |
| 656 |
2 |
395.2362 | 788.4578 | 788.4545 | 4.27 | 0 | 27 | 0.0059 | 1Score > 17 indicates identity |
U | R.IFGPVTR.E |
| 676 |
400.7178 | 799.4210 | 799.4188 | 2.80 | 0 | 32 | 0.0028 | 1Score > 19 indicates identity |
U | K.QEPIGTR.I | |
| 878 |
444.2583 | 886.5020 | 886.4984 | 4.07 | 1 | 27 | 0.015 | 1Score > 21 indicates identity |
U | R.RADGSIIR.F | |
| 1010 |
1 |
940.5779 | 939.5706 | 939.5641 | 6.97 | 1 | 31 | 0.0012 | 1Score > 14 indicates identity |
U | K.IVSLAPEVL.- |
| 1011 |
470.7928 | 939.5710 | 939.5641 | 7.42 | 1 | 26 | 0.0035 | 1Score > 14 indicates identity |
U | K.IVSLAPEVL.- | |
| 1045 |
949.5393 | 948.5320 | 948.5280 | 4.23 | 0 | 56 | 9.1e-006 | 1Score > 18 indicates identity |
U | R.YAGIGDIIK.V | |
| 1046 |
1 |
475.2741 | 948.5336 | 948.5280 | 5.94 | 0 | 39 | 0.00034 | 1Score > 17 indicates identity |
U | R.YAGIGDIIK.V |
| 1266 |
530.3098 | 1058.6050 | 1058.5907 | 13.6 | 0 | 35 | 0.001 | 1Score > 18 indicates identity |
U | K.GQVMTAVVVR.T | |
| 1736 |
1 |
638.3443 | 1274.6740 | 1274.6619 | 9.55 | 0 | 67 | 1.5e-006 | 1Score > 21 indicates identity |
U | R.FDGNAAVLLNNK.Q |
48| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88MH2_PSEPK | 260 | Outer membrane protein assembly factor BamA OS=Pseudomonas putida (strain KT2440) GN=bamA PE=3 SV=1 |
49| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88PJ4_PSEPK | 259 | Outer membrane protein assembly factor BamB OS=Pseudomonas putida (strain KT2440) GN=bamB PE=3 SV=1 |
50| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88FM2_PSEPK | 258 | Acetyl-CoA carboxylase, biotin carboxylase, putative OS=Pseudomonas putida (strain KT2440) GN=PP_4067 PE=4 SV=1 |
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