MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita1 |
| MS data file | : | PRT1270_T-BRSC_1_20250714114344.mgf |
| Database | : | P-putida 20180924 (5,556 sequences; 1,892,173 residues) |
| Timestamp | : | 12 Aug 2025 at 22:47:09 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,938 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
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41| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88DV1_PSEPK | 291 | ATP-dependent zinc metalloprotease FtsH OS=Pseudomonas putida (strain KT2440) GN=ftsH PE=3 SV=1 |
42| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ATPF_PSEPK | 287 | ATP synthase subunit b OS=Pseudomonas putida (strain KT2440) GN=atpF PE=3 SV=1 |
43| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RS9_PSEPK | 277 | 30S ribosomal protein S9 OS=Pseudomonas putida (strain KT2440) GN=rpsI PE=3 SV=1 |
44| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL18_PSEPK | 277 | 50S ribosomal protein L18 OS=Pseudomonas putida (strain KT2440) GN=rplR PE=3 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 44.1 | RL18_PSEPK |
277 | 12677 | 10 (6) | 7 (5) | 1.83 | |
| 50S ribosomal protein L18 OS=Pseudomonas putida (strain KT2440) GN=rplR PE=3 SV=1 | |||||||
10 peptide matches (10 non-duplicate, 0 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 287 |
587.3529 | 586.3456 | 586.3438 | 3.04 | 1 | 5 | 1.2 | 4Score > 25 indicates identity Score > 19 indicates homology |
K.LVAER.A | ||
| 441 |
344.1936 | 686.3726 | 686.3711 | 2.24 | 0 | 21 | 0.096 | 1Score > 24 indicates identity |
U | K.ALADAAR.E | |
| 442 |
687.3805 | 686.3732 | 686.3711 | 3.08 | 0 | 13 | 0.62 | 6Score > 24 indicates identity |
U | K.ALADAAR.E | |
| 1160 |
1004.5444 | 1003.5371 | 1003.5550 | -17.8 | 0 | 17 | 0.16 | 2Score > 21 indicates identity |
U | K.VLASASTLDK.D | |
| 1161 |
502.7768 | 1003.5390 | 1003.5550 | -15.9 | 0 | 23 | 0.033 | 2Score > 21 indicates identity |
U | K.VLASASTLDK.D | |
| 1389 |
560.7926 | 1119.5706 | 1119.5673 | 3.01 | 0 | 61 | 7.1e-006 | 1Score > 22 indicates identity |
U | K.AAGVSQVAFDR.S | |
| 1431 |
567.2758 | 1132.5370 | 1132.5360 | 0.90 | 0 | 75 | 2.2e-007 | 1Score > 21 indicates identity |
U | R.DGATGNIDAATK.V | |
| 1957 |
694.8934 | 1387.7722 | 1387.7671 | 3.73 | 1 | 104 | 1.4e-010 | 1Score > 18 indicates identity |
U | K.VLASASTLDKDLR.D | |
| 2609 |
564.2859 | 1689.8359 | 1689.8322 | 2.17 | 0 | 44 | 0.00029 | 1Score > 21 indicates identity |
U | R.SSQHIYAQVISADGSK.V | |
| 2610 |
845.9254 | 1689.8362 | 1689.8322 | 2.40 | 0 | 74 | 2.9e-007 | 1Score > 21 indicates identity |
U | R.SSQHIYAQVISADGSK.V |
45| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88KF1_PSEPK | 276 | Aconitate hydratase B OS=Pseudomonas putida (strain KT2440) GN=acnB PE=3 SV=1 |
46| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88Q24_PSEPK | 275 | ABC transporter, ATP-binding protein OS=Pseudomonas putida (strain KT2440) GN=PP_0674 PE=3 SV=1 |
47| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL14_PSEPK | 274 | 50S ribosomal protein L14 OS=Pseudomonas putida (strain KT2440) GN=rplN PE=3 SV=1 |
48| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88MH2_PSEPK | 260 | Outer membrane protein assembly factor BamA OS=Pseudomonas putida (strain KT2440) GN=bamA PE=3 SV=1 |
49| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88PJ4_PSEPK | 259 | Outer membrane protein assembly factor BamB OS=Pseudomonas putida (strain KT2440) GN=bamB PE=3 SV=1 |
50| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88FM2_PSEPK | 258 | Acetyl-CoA carboxylase, biotin carboxylase, putative OS=Pseudomonas putida (strain KT2440) GN=PP_4067 PE=4 SV=1 |
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