MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 12 Aug 2025 at 22:47:09 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 41–50 (out of 313)

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+41

Accession Score Description
1 Q88DV1_PSEPK 291 ATP-dependent zinc metalloprotease FtsH OS=Pseudomonas putida (strain KT2440) GN=ftsH PE=3 SV=1

+42

Accession Score Description
1 ATPF_PSEPK 287 ATP synthase subunit b OS=Pseudomonas putida (strain KT2440) GN=atpF PE=3 SV=1

-43

Accession Score Description
1 RS9_PSEPK 277 30S ribosomal protein S9 OS=Pseudomonas putida (strain KT2440) GN=rpsI PE=3 SV=1
Score Mass Matches Sequences emPAI
43.1 RS9_PSEPK 277 14583 13 (12) 5 (5) 2.90
30S ribosomal protein S9 OS=Pseudomonas putida (strain KT2440) GN=rpsI PE=3 SV=1

-13 peptide matches (9 non-duplicate, 4 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
394   333.6828 665.3510 665.3497 2.07 0 32 0.0038 +1Score > 20 indicates identity U R.AGYVTR.D
395 +1 666.3594 665.3521 665.3497 3.69 0 23 0.024 +2Score > 19 indicates identity U R.AGYVTR.D
1004 +2 470.7502 939.4858 939.4814 4.70 0 50 4.7e-005 +1Score > 19 indicates identity U R.SLDVFFGR.E
1006   940.4968 939.4895 939.4814 8.62 0 37 0.00082 +1Score > 19 indicates identity U R.SLDVFFGR.E
1254   527.7517 1053.4888 1053.4839 4.67 0 68 7.8e-007 +1Score > 19 indicates identity U M.SATQNYGTGR.R
2536 +1 553.3102 1656.9088 1656.9060 1.69 0 39 0.00041 +1Score > 18 indicates identity U R.VFLRPGTGNISINNR.S
2537   829.4620 1656.9094 1656.9060 2.10 0 51 3.1e-005 +1Score > 18 indicates identity U R.VFLRPGTGNISINNR.S
2540   553.6454 1657.9144 1657.8900 14.7 0 22 0.021 +1Score > 18 indicates identity U R.VFLRPGTGNISINNR.S + Deamidated (NQ)
3082   977.5109 1953.0072 1952.9956 5.95 0 75 1.9e-007 +1Score > 20 indicates identity U K.FDIYVTVSGGGVSGQAGAIR.H

+44

Accession Score Description
1 RL18_PSEPK 277 50S ribosomal protein L18 OS=Pseudomonas putida (strain KT2440) GN=rplR PE=3 SV=1

+45

Accession Score Description
1 Q88KF1_PSEPK 276 Aconitate hydratase B OS=Pseudomonas putida (strain KT2440) GN=acnB PE=3 SV=1

+46

Accession Score Description
1 Q88Q24_PSEPK 275 ABC transporter, ATP-binding protein OS=Pseudomonas putida (strain KT2440) GN=PP_0674 PE=3 SV=1

+47

Accession Score Description
1 RL14_PSEPK 274 50S ribosomal protein L14 OS=Pseudomonas putida (strain KT2440) GN=rplN PE=3 SV=1

+48

Accession Score Description
1 Q88MH2_PSEPK 260 Outer membrane protein assembly factor BamA OS=Pseudomonas putida (strain KT2440) GN=bamA PE=3 SV=1

+49

Accession Score Description
1 Q88PJ4_PSEPK 259 Outer membrane protein assembly factor BamB OS=Pseudomonas putida (strain KT2440) GN=bamB PE=3 SV=1

+50

Accession Score Description
1 Q88FM2_PSEPK 258 Acetyl-CoA carboxylase, biotin carboxylase, putative OS=Pseudomonas putida (strain KT2440) GN=PP_4067 PE=4 SV=1
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