MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 12 Aug 2025 at 22:47:09 GMT
Repeat search

[help]

Export

Not what you expected? Try the select summary.

Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

Filters

[help]

Show

Protein families 31–40 (out of 313)

Page: Previous 1 2 3 4 5 6 7 8 9 … 32 Next 

+31

Accession Score Description
Family member distances as a dendrogram 1 ATPG_PSEPK 335 ATP synthase gamma chain OS=Pseudomonas putida (strain KT2440) GN=atpG PE=3 SV=1
2 Q88MY8_PSEPK 23 description

+32

Accession Score Description
1 RPOA_PSEPK 333 DNA-directed RNA polymerase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=rpoA PE=3 SV=1

+33

Accession Score Description
1 RS3_PSEPK 311 30S ribosomal protein S3 OS=Pseudomonas putida (strain KT2440) GN=rpsC PE=3 SV=2

+34

Accession Score Description
1 Q88E12_PSEPK 307 Protocatechuate 3,4-dioxygenase, beta subunit OS=Pseudomonas putida (strain KT2440) GN=pcaH PE=4 SV=1

+35

Accession Score Description
1 Q88PS5_PSEPK 302 OmpA family protein OS=Pseudomonas putida (strain KT2440) GN=PP_0773 PE=3 SV=1

+36

Accession Score Description
1 RS16_PSEPK 299 30S ribosomal protein S16 OS=Pseudomonas putida (strain KT2440) GN=rpsP PE=3 SV=1

+37

Accession Score Description
1 TTGB_PSEPK 299 Probable efflux pump membrane transporter TtgB OS=Pseudomonas putida (strain KT2440) GN=ttgB PE=1 SV=1

+38

Accession Score Description
1 RL11_PSEPK 295 50S ribosomal protein L11 OS=Pseudomonas putida (strain KT2440) GN=rplK PE=3 SV=1

+39

Accession Score Description
1 RS4_PSEPK 295 30S ribosomal protein S4 OS=Pseudomonas putida (strain KT2440) GN=rpsD PE=3 SV=1

-40

Accession Score Description
1 TTGA_PSEPK 292 Probable efflux pump periplasmic linker TtgA OS=Pseudomonas putida (strain KT2440) GN=ttgA PE=1 SV=1
Score Mass Matches Sequences emPAI
40.1 TTGA_PSEPK 292 41282 14 (9) 13 (8) 0.63
Probable efflux pump periplasmic linker TtgA OS=Pseudomonas putida (strain KT2440) GN=ttgA PE=1 SV=1

-14 peptide matches (14 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
392   333.6772 665.3398 665.3384 2.12 0 3 1.3 +2Score > 20 indicates identity
Score > 17 indicates homology		 U E.DGSLFK.Q
584   379.7310 757.4474 757.4446 3.75 0 26 0.033 +1Score > 23 indicates identity U K.ANLLATR.S
692   803.4277 802.4204 802.4185 2.45 0 10 1.4 +10Score > 24 indicates identity U K.SAQIDLR.Y + Deamidated (NQ)
701   406.7540 811.4934 811.4916 2.31 0 47 4.3e-005 +1Score > 16 indicates identity U K.VLAPISGR.I
821   430.2484 858.4822 858.4810 1.40 1 1 6.8 +5Score > 22 indicates identity U R.LQAEASLK.S
1186   509.2655 1016.5164 1016.5138 2.58 1 24 0.02 +1Score > 22 indicates identity
Score > 20 indicates homology		 U R.DLESGQLQK.A
1426   566.2876 1130.5606 1130.5819 -18.8 1 24 0.036 +2Score > 22 indicates identity U K.QLIDEQAVSK.Q + Deamidated (NQ)
1851   670.8647 1339.7148 1339.7096 3.94 1 61 4e-006 +1Score > 20 indicates identity U K.GAPTALVVNQENK.V
2266   512.9841 1535.9305 1535.9035 17.6 1 7 0.19 +1Score > 13 indicates identity U R.VAEVRPQVNGIILK.R + Deamidated (NQ)
2597   840.4648 1678.9150 1678.9115 2.14 0 68 5e-007 +1Score > 17 indicates identity U K.AGVNANAILAPQQGVTR.D
3060   645.3377 1932.9913 1932.9793 6.21 1 48 3.4e-005 +1Score > 20 indicates identity
Score > 16 indicates homology		 U K.AGDNAASVQLVLEDGSLFK.Q
3061   967.5037 1932.9928 1932.9793 7.03 1 98 8.2e-010 +1Score > 20 indicates identity U K.AGDNAASVQLVLEDGSLFK.Q
3406   534.2875 2133.1209 2133.0830 17.8 0 48 5.6e-005 +1Score > 18 indicates identity U R.ALFPNPDHTLLPGMFVHAR.L + Deamidated (NQ)
3531   1097.5460 2193.0774 2193.0953 -8.15 1 5 2 +2Score > 21 indicates identity U E.GQQLYQIDPAVYEATLANAK.A + Deamidated (NQ)
Page: Previous 1 2 3 4 5 6 7 8 9 … 32 Next 

Not what you expected? Try the select summary.

Mascot: http://www.matrixscience.com/