MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 12 Aug 2025 at 22:47:09 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 1–10 (out of 313)

Page: 1 2 3 4 5 6 … 32 Next 

+1

Accession Score Description
1 Q88NM2_PSEPK 4307 Outer membrane protein H1 OS=Pseudomonas putida (strain KT2440) GN=oprH PE=4 SV=1

+2

Accession Score Description
Family member distances as a dendrogram 1 EFTU2_PSEPK 2609 Elongation factor Tu-B OS=Pseudomonas putida (strain KT2440) GN=tufB PE=3 SV=1
2 EFTU1_PSEPK 2412 Elongation factor Tu-A OS=Pseudomonas putida (strain KT2440) GN=tufA PE=1 SV=1

+3

Accession Score Description
1 ATPB_PSEPK 2344 ATP synthase subunit beta OS=Pseudomonas putida (strain KT2440) GN=atpD PE=3 SV=1

-4

Accession Score Description
1 Q88ES5_PSEPK 2322 Flagellin OS=Pseudomonas putida (strain KT2440) GN=fliC PE=3 SV=1
Score Mass Matches Sequences emPAI
4.1 Q88ES5_PSEPK 2322 67806 49 (42) 20 (19) 1.79
Flagellin OS=Pseudomonas putida (strain KT2440) GN=fliC PE=3 SV=1

-49 peptide matches (37 non-duplicate, 12 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
307   604.3674 603.3601 603.3592 1.60 0 40 0.00026 +1Score > 16 indicates identity U R.LSSGLK.I
308   302.6877 603.3608 603.3592 2.80 0 24 0.0098 +1Score > 16 indicates identity U R.LSSGLK.I
D:\Xcalibur\Data\Jennifer\PRT1270 Rita DDA\PRT1270_T-BRSC_1_20250714114344.raw

Score > 18 indicates identity
419   337.2169 672.4192 672.4170 3.31 0 23 0.016 -1Score > 18 indicates identity U K.GLSVAVK.N
18.1 1 8 0.52 2 AWIKR  
18.1 1 8 0.52 2 AWLKR  
-13.4 1 7 0.75 4 SLLGKR  
3.31 0 7 0.75 5 SLVQVK  
3.33 1 4 1.2 6 IGDLKK  
-13.4 1 4 1.4 7 GLISRK  
3.33 1 4 1.4 8 QKVLGK   + Deamidated (NQ)
-13.4 1 3 1.7 9 SLARVK  
3.33 0 3 1.7 10 AITAAVK  
420   673.4290 672.4217 672.4170 6.99 0 44 9.7e-005 +1Score > 17 indicates identity U K.GLSVAVK.N
481 +1 352.2224 702.4302 702.4276 3.80 0 10 0.17 +1Score > 15 indicates identity U R.LTTQIK.G
482   703.4376 702.4303 702.4276 3.90 0 24 0.0057 +1Score > 15 indicates identity U R.LTTQIK.G
535   366.2067 730.3988 730.3973 2.07 0 12 0.93 +3Score > 24 indicates identity U E.AGGAQLSK.T
538   732.4019 731.3946 731.3926 2.78 0 23 0.062 +1Score > 23 indicates identity U K.TGTANLR.D
539   366.7047 731.3948 731.3926 3.08 0 48 0.00021 +1Score > 23 indicates identity U K.TGTANLR.D
1094   972.5245 971.5172 971.5148 2.46 0 43 0.00046 +1Score > 22 indicates identity U R.SQLGAVQNR.F
1095   486.7660 971.5174 971.5148 2.69 0 38 0.0008 +1Score > 22 indicates identity
Score > 19 indicates homology		 U R.SQLGAVQNR.F
1230   519.2740 1036.5334 1036.5302 3.17 0 66 1.5e-006 +1Score > 20 indicates identity U R.IANTTTFGGR.N
1342   548.2604 1094.5062 1094.5026 3.31 0 58 9.3e-006 +1Score > 20 indicates identity U K.SSDALGTTMGR.L
1497   580.2903 1158.5660 1158.5629 2.72 0 51 6e-005 +1Score > 22 indicates identity
Score > 21 indicates homology		 U K.DDAAGLQISNR.L
1705 +1 629.8559 1257.6972 1257.6929 3.49 0 79 6e-008 +1Score > 19 indicates identity U R.LVLTSANGQDIK.L
1708   630.3486 1258.6826 1258.6769 4.59 0 46 0.00015 +1Score > 20 indicates identity U R.LVLTSANGQDIK.L + Deamidated (NQ)
2399   536.2835 1605.8287 1605.8396 -6.81 0 13 0.26 +1Score > 19 indicates identity U M.ALTVNTNITSMSVQK.N
2403   803.9313 1605.8480 1605.8396 5.26 0 84 1.8e-008 +1Score > 19 indicates identity U M.ALTVNTNITSMSVQK.N
2584 +1 836.9656 1671.9166 1671.9043 7.38 1 88 5.8e-009 +1Score > 18 indicates identity U K.AGLVADTSISLNGVEVK.F
2585   558.3132 1671.9178 1671.9043 8.05 1 18 0.054 +1Score > 18 indicates identity U K.AGLVADTSISLNGVEVK.F
2587   558.6278 1672.8616 1672.8380 14.1 1 52 2.7e-005 +1Score > 21 indicates identity
Score > 18 indicates homology		 U K.INSAKDDAAGLQISNR.L + Deamidated (NQ)
2588 +1 837.4651 1672.9156 1672.8883 16.3 1 78 4.6e-008 +1Score > 17 indicates identity U K.AGLVADTSISLNGVEVK.F + Deamidated (NQ)
2611   846.3784 1690.7422 1690.7394 1.68 1 122 1.5e-012 +1Score > 17 indicates identity U R.ELALQSANGSNSDDDR.A
3170   670.9976 2009.9710 2009.9654 2.78 1 21 0.038 +1Score > 20 indicates identity U R.FDSTVANLQSISENSTAAR.S
3171   1005.9938 2009.9730 2009.9654 3.81 1 109 6.9e-011 +1Score > 20 indicates identity U R.FDSTVANLQSISENSTAAR.S
3175   1006.4918 2010.9690 2010.9494 9.77 1 114 2.4e-011 +1Score > 20 indicates identity U R.FDSTVANLQSISENSTAAR.S + Deamidated (NQ)
3176   671.3322 2010.9748 2010.9494 12.6 1 82 3.4e-008 +1Score > 20 indicates identity U R.FDSTVANLQSISENSTAAR.S + Deamidated (NQ)
3177   1006.4968 2010.9790 2010.9494 14.7 1 91 4.3e-009 +1Score > 20 indicates identity U R.FDSTVANLQSISENSTAAR.S + Deamidated (NQ)
3380   707.3687 2119.0843 2119.0757 4.04 0 90 5.3e-009 +1Score > 20 indicates identity U K.VNAQTGTTGVTASIDSATGQLK.L
3381 +1 1061.0498 2120.0850 2120.0597 11.9 0 144 2.2e-014 +1Score > 20 indicates identity U K.VNAQTGTTGVTASIDSATGQLK.L + Deamidated (NQ)
3513 +1 1095.0631 2188.1116 2188.0972 6.62 0 129 7.1e-013 +1Score > 20 indicates identity U K.LDNGSGVTGQGALAAVGLNSGTTK.A + Deamidated (NQ)
3514 +1 730.3787 2188.1143 2188.0972 7.82 0 49 6.7e-005 +1Score > 20 indicates identity U K.LDNGSGVTGQGALAAVGLNSGTTK.A + Deamidated (NQ)
3668   1147.5818 2293.1490 2293.1186 13.3 1 121 3.6e-012 +1Score > 19 indicates identity U K.GNYNEASVAGGVATLQASVTGAAGK.F + Deamidated (NQ)
3669   1147.5830 2293.1514 2293.1186 14.3 1 141 3.8e-014 +1Score > 19 indicates identity U K.GNYNEASVAGGVATLQASVTGAAGK.F + Deamidated (NQ)
3671 +1 765.3922 2293.1548 2293.1186 15.8 1 100 5e-010 +1Score > 19 indicates identity U K.GNYNEASVAGGVATLQASVTGAAGK.F + Deamidated (NQ)
4354 +2 1010.5552 3028.6438 3028.5929 16.8 0 102 7.1e-011 +1Score > 13 indicates identity U R.DGVLGAGGAGNLTLGSTNIALVATDTLSSVVGK.V + Deamidated (NQ)
4516 +2 1079.8918 3236.6536 3236.5936 18.5 1 92 1.5e-009 +1Score > 16 indicates identity U K.LDGTQAFTIDASTDDVLSTALGLAEAGGAQLSK.T + Deamidated (NQ)

+5

Accession Score Description
1 Q88L46_PSEPK 1745 Outer membrane protein OprF OS=Pseudomonas putida (strain KT2440) GN=oprF PE=3 SV=1

+6

Accession Score Description
1 ATPA_PSEPK 1711 ATP synthase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=atpA PE=3 SV=1

+7

Accession Score Description
1 Q88FB0_PSEPK 1295 Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex OS=Pseudomonas putida (strain KT2440) GN=kgdB PE=3 SV=1

+8

Accession Score Description
1 Q88FA7_PSEPK 1233 Succinate dehydrogenase flavoprotein subunit OS=Pseudomonas putida (strain KT2440) GN=sdhA PE=3 SV=1

+9

Accession Score Description
1 RPOB_PSEPK 843 DNA-directed RNA polymerase subunit beta OS=Pseudomonas putida (strain KT2440) GN=rpoB PE=3 SV=1

+10

Accession Score Description
1 Q88FA8_PSEPK 775 Succinate dehydrogenase, iron-sulfur protein OS=Pseudomonas putida (strain KT2440) GN=sdhB PE=4 SV=1
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