MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 12 Aug 2025 at 22:47:09 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 31–40 (out of 313)

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+31

Accession Score Description
Family member distances as a dendrogram 1 ATPG_PSEPK 335 ATP synthase gamma chain OS=Pseudomonas putida (strain KT2440) GN=atpG PE=3 SV=1
2 Q88MY8_PSEPK 23 description

+32

Accession Score Description
1 RPOA_PSEPK 333 DNA-directed RNA polymerase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=rpoA PE=3 SV=1

+33

Accession Score Description
1 RS3_PSEPK 311 30S ribosomal protein S3 OS=Pseudomonas putida (strain KT2440) GN=rpsC PE=3 SV=2

+34

Accession Score Description
1 Q88E12_PSEPK 307 Protocatechuate 3,4-dioxygenase, beta subunit OS=Pseudomonas putida (strain KT2440) GN=pcaH PE=4 SV=1

+35

Accession Score Description
1 Q88PS5_PSEPK 302 OmpA family protein OS=Pseudomonas putida (strain KT2440) GN=PP_0773 PE=3 SV=1

+36

Accession Score Description
1 RS16_PSEPK 299 30S ribosomal protein S16 OS=Pseudomonas putida (strain KT2440) GN=rpsP PE=3 SV=1

+37

Accession Score Description
1 TTGB_PSEPK 299 Probable efflux pump membrane transporter TtgB OS=Pseudomonas putida (strain KT2440) GN=ttgB PE=1 SV=1

+38

Accession Score Description
1 RL11_PSEPK 295 50S ribosomal protein L11 OS=Pseudomonas putida (strain KT2440) GN=rplK PE=3 SV=1

-39

Accession Score Description
1 RS4_PSEPK 295 30S ribosomal protein S4 OS=Pseudomonas putida (strain KT2440) GN=rpsD PE=3 SV=1
Score Mass Matches Sequences emPAI
39.1 RS4_PSEPK 295 23270 30 (13) 15 (9) 2.14
30S ribosomal protein S4 OS=Pseudomonas putida (strain KT2440) GN=rpsD PE=3 SV=1

-30 peptide matches (20 non-duplicate, 10 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
247   537.3058 536.2985 536.2959 4.98 0 2 2 +1Score > 17 indicates identity U K.SGVFK.N
257   556.3223 555.3150 555.3129 3.86 0 7 0.32 +6Score > 15 indicates identity U K.NVPAR.S
270   577.3359 576.3286 576.3271 2.59 0 23 0.013 +1Score > 17 indicates identity U R.YIGPK.C
416 +1 337.2038 672.3930 672.3919 1.77 0 18 0.19 +3Score > 23 indicates identity U K.SLGQLR.I
446   344.7050 687.3954 687.3915 5.71 0 21 0.061 +1Score > 22 indicates identity U K.AISVNGK.T
447   345.1949 688.3752 688.3755 -0.42 0 12 0.56 +3Score > 22 indicates identity U K.AISVNGK.T + Deamidated (NQ)
690 +3 401.2520 800.4894 800.4868 3.29 1 13 0.25 +3Score > 20 indicates identity U E.KSLGQLR.I
691 +2 801.4975 800.4902 800.4868 4.26 1 13 0.28 +2Score > 20 indicates identity U E.KSLGQLR.I
785   849.4850 848.4777 848.4756 2.52 1 31 0.0039 +1Score > 20 indicates identity U R.IYGVLER.Q
786   425.2464 848.4782 848.4756 3.14 1 26 0.0038 +1Score > 20 indicates identity
Score > 15 indicates homology		 U R.IYGVLER.Q
856   439.7417 877.4688 877.4658 3.52 0 46 0.00021 +1Score > 21 indicates identity U R.LDNVVYR.M
962 +2 461.7491 921.4836 921.4807 3.15 1 53 3.3e-005 +1Score > 20 indicates identity U R.EGTDLFLK.S
1067   958.4661 957.4588 957.4556 3.40 0 43 0.00031 +1Score > 20 indicates identity U R.GYYQAAASK.K
1068   479.7367 957.4588 957.4556 3.43 0 38 0.001 +1Score > 20 indicates identity U R.GYYQAAASK.K
1279   534.2614 1066.5082 1066.5043 3.67 0 66 1.3e-006 +1Score > 20 indicates identity U R.QSDYGTQLR.E
1427   566.2895 1130.5644 1130.5608 3.23 1 56 2.6e-005 +1Score > 22 indicates identity U R.VEWVDVDAAK.K
1786   650.8594 1299.7042 1299.6969 5.67 1 40 0.00066 +1Score > 21 indicates identity U R.IVQALELCAQR.G
1938   461.5934 1381.7584 1381.7565 1.35 1 3 1.8 +1Score > 18 indicates identity U R.QLVSHKAISVNGK.T + 2 Deamidated (NQ)
3099   985.5500 1969.0854 1969.0745 5.55 0 58 3.4e-006 +1Score > 16 indicates identity U K.TVNIPSYQVRPGDVVAVR.E
3101 +2 657.3697 1969.0873 1969.0745 6.47 0 19 0.025 +1Score > 16 indicates identity U K.TVNIPSYQVRPGDVVAVR.E

+40

Accession Score Description
1 TTGA_PSEPK 292 Probable efflux pump periplasmic linker TtgA OS=Pseudomonas putida (strain KT2440) GN=ttgA PE=1 SV=1
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