MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 12 Aug 2025 at 22:47:09 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 31–40 (out of 313)

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+31

Accession Score Description
Family member distances as a dendrogram 1 ATPG_PSEPK 335 ATP synthase gamma chain OS=Pseudomonas putida (strain KT2440) GN=atpG PE=3 SV=1
2 Q88MY8_PSEPK 23 description

+32

Accession Score Description
1 RPOA_PSEPK 333 DNA-directed RNA polymerase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=rpoA PE=3 SV=1

+33

Accession Score Description
1 RS3_PSEPK 311 30S ribosomal protein S3 OS=Pseudomonas putida (strain KT2440) GN=rpsC PE=3 SV=2

+34

Accession Score Description
1 Q88E12_PSEPK 307 Protocatechuate 3,4-dioxygenase, beta subunit OS=Pseudomonas putida (strain KT2440) GN=pcaH PE=4 SV=1

+35

Accession Score Description
1 Q88PS5_PSEPK 302 OmpA family protein OS=Pseudomonas putida (strain KT2440) GN=PP_0773 PE=3 SV=1

+36

Accession Score Description
1 RS16_PSEPK 299 30S ribosomal protein S16 OS=Pseudomonas putida (strain KT2440) GN=rpsP PE=3 SV=1

+37

Accession Score Description
1 TTGB_PSEPK 299 Probable efflux pump membrane transporter TtgB OS=Pseudomonas putida (strain KT2440) GN=ttgB PE=1 SV=1

-38

Accession Score Description
1 RL11_PSEPK 295 50S ribosomal protein L11 OS=Pseudomonas putida (strain KT2440) GN=rplK PE=3 SV=1
Score Mass Matches Sequences emPAI
38.1 RL11_PSEPK 295 14914 10 (8) 6 (6) 2.27
50S ribosomal protein L11 OS=Pseudomonas putida (strain KT2440) GN=rplK PE=3 SV=1

-10 peptide matches (8 non-duplicate, 2 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
820   430.2148 858.4150 858.4123 3.16 1 33 0.00087 +1Score > 21 indicates identity
Score > 15 indicates homology		 U R.SFTFETK.S
876 +1 444.2476 886.4806 886.4760 5.28 1 50 8.3e-005 +1Score > 22 indicates identity U R.AQLEDIAK.A
946   915.5532 914.5459 914.5437 2.46 0 41 0.00033 +1Score > 19 indicates identity U K.STPASVLLK.K
947   458.2817 914.5488 914.5437 5.66 0 24 0.012 +1Score > 18 indicates identity U K.STPASVLLK.K
2044   715.3705 1428.7264 1428.7209 3.91 0 88 1.3e-008 +1Score > 22 indicates identity U K.QADLTAADLDAAVR.T
2045   477.2649 1428.7729 1428.7685 3.08 0 26 0.014 +1Score > 20 indicates identity U K.AAGLTSGSARPNTVK.V
3433   1073.5706 2145.1266 2145.0954 14.6 1 93 1.6e-009 +1Score > 18 indicates identity U R.TQGQEAGLPTPVIITVYSDR.S + Deamidated (NQ)
3434 +1 716.0533 2145.1381 2145.0954 19.9 1 71 2.5e-007 +1Score > 17 indicates identity U R.TQGQEAGLPTPVIITVYSDR.S + Deamidated (NQ)

+39

Accession Score Description
1 RS4_PSEPK 295 30S ribosomal protein S4 OS=Pseudomonas putida (strain KT2440) GN=rpsD PE=3 SV=1

+40

Accession Score Description
1 TTGA_PSEPK 292 Probable efflux pump periplasmic linker TtgA OS=Pseudomonas putida (strain KT2440) GN=ttgA PE=1 SV=1
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