MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 12 Aug 2025 at 22:47:09 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 31–40 (out of 313)

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+31

Accession Score Description
Family member distances as a dendrogram 1 ATPG_PSEPK 335 ATP synthase gamma chain OS=Pseudomonas putida (strain KT2440) GN=atpG PE=3 SV=1
2 Q88MY8_PSEPK 23 description

+32

Accession Score Description
1 RPOA_PSEPK 333 DNA-directed RNA polymerase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=rpoA PE=3 SV=1

+33

Accession Score Description
1 RS3_PSEPK 311 30S ribosomal protein S3 OS=Pseudomonas putida (strain KT2440) GN=rpsC PE=3 SV=2

+34

Accession Score Description
1 Q88E12_PSEPK 307 Protocatechuate 3,4-dioxygenase, beta subunit OS=Pseudomonas putida (strain KT2440) GN=pcaH PE=4 SV=1

+35

Accession Score Description
1 Q88PS5_PSEPK 302 OmpA family protein OS=Pseudomonas putida (strain KT2440) GN=PP_0773 PE=3 SV=1

+36

Accession Score Description
1 RS16_PSEPK 299 30S ribosomal protein S16 OS=Pseudomonas putida (strain KT2440) GN=rpsP PE=3 SV=1

-37

Accession Score Description
1 TTGB_PSEPK 299 Probable efflux pump membrane transporter TtgB OS=Pseudomonas putida (strain KT2440) GN=ttgB PE=1 SV=1
Score Mass Matches Sequences emPAI
37.1 TTGB_PSEPK 299 112935 13 (10) 12 (9) 0.22
Probable efflux pump membrane transporter TtgB OS=Pseudomonas putida (strain KT2440) GN=ttgB PE=1 SV=1

-13 peptide matches (13 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
502   358.2283 714.4420 714.4388 4.53 0 12 0.4 +5Score > 20 indicates identity U R.SVGAILR.N
509   360.7031 719.3916 719.3966 -6.89 0 4 2.2 +5Score > 20 indicates identity U R.NQFLAK.A
764   421.7436 841.4726 841.4698 3.42 0 29 0.0044 +1Score > 18 indicates identity U R.IWLDPAK.L
1246   523.8008 1045.5870 1045.5808 5.97 0 18 0.025 +1Score > 20 indicates identity
Score > 15 indicates homology		 U K.FQLTPVDVK.T
1328   544.2642 1086.5138 1086.5036 9.46 0 28 0.004 +1Score > 20 indicates identity
Score > 16 indicates homology		 U K.GGFFGWFNR.N
1461   573.3132 1144.6118 1144.6088 2.68 0 48 0.00012 +1Score > 21 indicates identity U K.LATGANALDTAK.A
1550   394.5345 1180.5817 1180.5778 3.29 0 28 0.0098 +1Score > 20 indicates identity U R.AQQHFFTFR.D
2148   495.9560 1484.8462 1484.8311 10.2 1 0 2.1 +5Score > 16 indicates identity U K.LATGANALDTAKALR.E
3188   1010.0805 2018.1464 2018.1160 15.1 1 74 4.8e-008 +1Score > 13 indicates identity U K.LNLATPLLPQEVQQQGIR.V + Deamidated (NQ)
3189   673.7239 2018.1499 2018.1160 16.8 1 69 1.3e-007 +1Score > 13 indicates identity U K.LNLATPLLPQEVQQQGIR.V + Deamidated (NQ)
4137   919.8059 2756.3959 2756.3617 12.4 1 35 0.001 +1Score > 18 indicates identity U K.ILSAVRPNGLNDEPQYQLTIDDER.A + Deamidated (NQ)
4398   1032.5490 3094.6252 3094.5822 13.9 0 59 2.8e-006 +1Score > 16 indicates identity U R.ASALGVTIADINNTLSIALGASYVNDFIDR.G + Deamidated (NQ)
4457   1059.2621 3174.7645 3174.7248 12.5 0 58 1.5e-006 +1Score > 13 indicates identity U K.TAVAAQNVQVSSGQLGGLPALPGTQLNATIIGK.T + Deamidated (NQ)

+38

Accession Score Description
1 RL11_PSEPK 295 50S ribosomal protein L11 OS=Pseudomonas putida (strain KT2440) GN=rplK PE=3 SV=1

+39

Accession Score Description
1 RS4_PSEPK 295 30S ribosomal protein S4 OS=Pseudomonas putida (strain KT2440) GN=rpsD PE=3 SV=1

+40

Accession Score Description
1 TTGA_PSEPK 292 Probable efflux pump periplasmic linker TtgA OS=Pseudomonas putida (strain KT2440) GN=ttgA PE=1 SV=1
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