MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 12 Aug 2025 at 22:47:09 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 31–40 (out of 313)

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+31

Accession Score Description
Family member distances as a dendrogram 1 ATPG_PSEPK 335 ATP synthase gamma chain OS=Pseudomonas putida (strain KT2440) GN=atpG PE=3 SV=1
2 Q88MY8_PSEPK 23 description

+32

Accession Score Description
1 RPOA_PSEPK 333 DNA-directed RNA polymerase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=rpoA PE=3 SV=1

-33

Accession Score Description
1 RS3_PSEPK 311 30S ribosomal protein S3 OS=Pseudomonas putida (strain KT2440) GN=rpsC PE=3 SV=2
Score Mass Matches Sequences emPAI
33.1 RS3_PSEPK 311 25709 31 (21) 13 (10) 4.16
30S ribosomal protein S3 OS=Pseudomonas putida (strain KT2440) GN=rpsC PE=3 SV=2

-31 peptide matches (24 non-duplicate, 7 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
218   501.3160 500.3087 500.3071 3.28 0 5 1.1 +2Score > 24 indicates identity
Score > 17 indicates homology		 U E.VIGGR.Q
239   529.3723 528.3650 528.3635 2.84 0 18 0.095 +1Score > 20 indicates identity U R.LGIVK.E
384 +1 330.1959 658.3772 658.3762 1.56 0 30 0.015 +1Score > 24 indicates identity U K.IQVSGR.L
386   659.3857 658.3784 658.3762 3.35 0 36 0.0023 +1Score > 22 indicates identity U K.IQVSGR.L
454   692.4158 691.4085 691.4057 4.04 0 19 0.036 +1Score > 17 indicates identity U K.VWIFK.G
456 +1 346.7126 691.4106 691.4057 7.11 0 20 0.03 +1Score > 17 indicates identity U K.VWIFK.G
579   377.6804 753.3462 753.3446 2.22 1 31 0.0025 +1Score > 18 indicates identity U R.TEWYR.E
580   754.3537 753.3464 753.3446 2.46 1 21 0.025 +1Score > 18 indicates identity U R.TEWYR.E
646   786.4484 785.4411 785.4395 2.03 1 34 0.0042 +1Score > 23 indicates identity U R.LGGAEIAR.T
647 +1 393.7281 785.4416 785.4395 2.70 1 32 0.0065 +1Score > 23 indicates identity U R.LGGAEIAR.T
652   789.4056 788.3983 788.3963 2.58 0 24 0.046 +1Score > 23 indicates identity U R.AVQNAMR.I
653   395.2068 788.3990 788.3963 3.50 0 16 0.28 +1Score > 23 indicates identity U R.AVQNAMR.I
665   795.3896 794.3823 794.3810 1.65 1 45 0.00022 +1Score > 20 indicates identity U R.EYLQDK.L
666   398.1987 794.3828 794.3810 2.31 1 23 0.033 +1Score > 20 indicates identity U R.EYLQDK.L
752   418.2626 834.5106 834.5076 3.69 0 33 0.00047 +1Score > 13 indicates identity U R.VPLHTLR.A
847   437.2628 872.5110 872.5080 3.55 1 19 0.063 +1Score > 20 indicates identity U K.LRQDLTK.Q
1738 +1 320.1830 1276.7029 1276.7000 2.27 0 23 0.018 +1Score > 18 indicates identity U R.IDIHRPAQTAR.I
1741   426.5754 1276.7044 1276.7000 3.43 0 18 0.052 +1Score > 17 indicates identity U R.IDIHRPAQTAR.I
1742   639.3599 1276.7052 1276.7000 4.11 0 26 0.0088 +1Score > 17 indicates identity U R.IDIHRPAQTAR.I
2127 +2 369.7334 1474.9045 1474.8984 4.15 0 9 0.12 +1Score > 13 indicates identity U R.ITIHTARPGIVIGK.K
2128   738.4596 1474.9046 1474.8984 4.25 0 31 0.00089 +1Score > 13 indicates identity U R.ITIHTARPGIVIGK.K
2129   492.6426 1474.9060 1474.8984 5.15 0 22 0.0062 +1Score > 13 indicates identity U R.ITIHTARPGIVIGK.K
3583   1116.0438 2230.0730 2230.0430 13.5 1 97 1e-009 +1Score > 19 indicates identity U R.ADIDYNTYEAHTTYGVIGVK.V + Deamidated (NQ)
3584 +1 744.3653 2230.0741 2230.0430 13.9 1 61 3.5e-006 +1Score > 19 indicates identity U R.ADIDYNTYEAHTTYGVIGVK.V + Deamidated (NQ)

+34

Accession Score Description
1 Q88E12_PSEPK 307 Protocatechuate 3,4-dioxygenase, beta subunit OS=Pseudomonas putida (strain KT2440) GN=pcaH PE=4 SV=1

+35

Accession Score Description
1 Q88PS5_PSEPK 302 OmpA family protein OS=Pseudomonas putida (strain KT2440) GN=PP_0773 PE=3 SV=1

+36

Accession Score Description
1 RS16_PSEPK 299 30S ribosomal protein S16 OS=Pseudomonas putida (strain KT2440) GN=rpsP PE=3 SV=1

+37

Accession Score Description
1 TTGB_PSEPK 299 Probable efflux pump membrane transporter TtgB OS=Pseudomonas putida (strain KT2440) GN=ttgB PE=1 SV=1

+38

Accession Score Description
1 RL11_PSEPK 295 50S ribosomal protein L11 OS=Pseudomonas putida (strain KT2440) GN=rplK PE=3 SV=1

+39

Accession Score Description
1 RS4_PSEPK 295 30S ribosomal protein S4 OS=Pseudomonas putida (strain KT2440) GN=rpsD PE=3 SV=1

+40

Accession Score Description
1 TTGA_PSEPK 292 Probable efflux pump periplasmic linker TtgA OS=Pseudomonas putida (strain KT2440) GN=ttgA PE=1 SV=1
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