MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 12 Aug 2025 at 22:47:09 GMT
Repeat search

[help]

Export

Not what you expected? Try the select summary.

Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

Filters

[help]

Show

Protein families 31–40 (out of 313)

Page: Previous 1 2 3 4 5 6 7 8 9 … 32 Next 

+31

Accession Score Description
Family member distances as a dendrogram 1 ATPG_PSEPK 335 ATP synthase gamma chain OS=Pseudomonas putida (strain KT2440) GN=atpG PE=3 SV=1
2 Q88MY8_PSEPK 23 description

-32

Accession Score Description
1 RPOA_PSEPK 333 DNA-directed RNA polymerase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=rpoA PE=3 SV=1
Score Mass Matches Sequences emPAI
32.1 RPOA_PSEPK 333 36721 17 (11) 11 (7) 0.84
DNA-directed RNA polymerase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=rpoA PE=3 SV=1

-17 peptide matches (15 non-duplicate, 2 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
344   629.3636 628.3563 628.3544 3.04 0 3 1.9 +9Score > 18 indicates identity U K.TPNLGK.K
497   713.4960 712.4887 712.4847 5.68 0 8 0.16 +4Score > 13 indicates identity U E.ILLNLK.G
498 +1 357.2518 712.4890 712.4847 6.13 0 10 0.11 +5Score > 13 indicates identity U E.ILLNLK.G
1092 +1 485.7845 969.5544 969.5495 5.14 1 41 0.00039 +1Score > 20 indicates identity
Score > 20 indicates homology		 U K.ITLEPLER.G
1219   517.7868 1033.5590 1033.5556 3.32 1 38 0.00078 +1Score > 19 indicates identity U R.IAYVVENAR.V
1449   570.8093 1139.6040 1139.5975 5.76 0 39 0.00062 +1Score > 19 indicates identity U R.LDNWPPASLK.K
1451   571.8083 1141.6020 1141.5992 2.46 0 36 0.0014 +1Score > 21 indicates identity
Score > 20 indicates homology		 U R.GFGHTLGNALR.R
1452   381.5413 1141.6021 1141.5992 2.48 0 41 0.00052 +1Score > 21 indicates identity U R.GFGHTLGNALR.R
1658   616.8403 1231.6660 1231.6561 8.09 0 59 6.7e-006 +1Score > 20 indicates identity U R.LQLDASFSPVR.R
1694   417.5680 1249.6822 1249.6779 3.42 0 35 0.00099 +1Score > 17 indicates identity U R.HIDVQVVSPTR.A
1695   625.8488 1249.6830 1249.6779 4.12 0 64 1.3e-006 +1Score > 17 indicates identity U R.HIDVQVVSPTR.A
1956   463.5959 1387.7659 1387.7572 6.26 1 12 0.25 +1Score > 18 indicates identity U R.LQLDASFSPVRR.I
2054   717.8631 1433.7116 1433.7337 -15.4 1 2 1 +1Score > 21 indicates identity
Score > 14 indicates homology		 U -.MQISVNEFLTPR.H
2323   523.2775 1566.8107 1566.8042 4.15 1 75 1.7e-007 +1Score > 20 indicates identity U K.AENIYYIGDLIQR.T
2324   784.4152 1566.8158 1566.8042 7.45 1 64 2e-006 +1Score > 20 indicates identity U K.AENIYYIGDLIQR.T

+33

Accession Score Description
1 RS3_PSEPK 311 30S ribosomal protein S3 OS=Pseudomonas putida (strain KT2440) GN=rpsC PE=3 SV=2

+34

Accession Score Description
1 Q88E12_PSEPK 307 Protocatechuate 3,4-dioxygenase, beta subunit OS=Pseudomonas putida (strain KT2440) GN=pcaH PE=4 SV=1

+35

Accession Score Description
1 Q88PS5_PSEPK 302 OmpA family protein OS=Pseudomonas putida (strain KT2440) GN=PP_0773 PE=3 SV=1

+36

Accession Score Description
1 RS16_PSEPK 299 30S ribosomal protein S16 OS=Pseudomonas putida (strain KT2440) GN=rpsP PE=3 SV=1

+37

Accession Score Description
1 TTGB_PSEPK 299 Probable efflux pump membrane transporter TtgB OS=Pseudomonas putida (strain KT2440) GN=ttgB PE=1 SV=1

+38

Accession Score Description
1 RL11_PSEPK 295 50S ribosomal protein L11 OS=Pseudomonas putida (strain KT2440) GN=rplK PE=3 SV=1

+39

Accession Score Description
1 RS4_PSEPK 295 30S ribosomal protein S4 OS=Pseudomonas putida (strain KT2440) GN=rpsD PE=3 SV=1

+40

Accession Score Description
1 TTGA_PSEPK 292 Probable efflux pump periplasmic linker TtgA OS=Pseudomonas putida (strain KT2440) GN=ttgA PE=1 SV=1
Page: Previous 1 2 3 4 5 6 7 8 9 … 32 Next 

Not what you expected? Try the select summary.

Mascot: http://www.matrixscience.com/