MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita1 |
| MS data file | : | PRT1270_T-BRSC_1_20250714114344.mgf |
| Database | : | P-putida 20180924 (5,556 sequences; 1,892,173 residues) |
| Timestamp | : | 12 Aug 2025 at 22:47:09 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,938 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
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301| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88CB4_PSEPK | 23 | Cytochrome c5 OS=Pseudomonas putida (strain KT2440) GN=PP_5267 PE=4 SV=1 |
302| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88QC1_PSEPK | 22 | Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_0573 PE=4 SV=1 |
303| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88EB3_PSEPK | 21 | RNA polymerase sigma-70 factor, ECF subfamily OS=Pseudomonas putida (strain KT2440) GN=PP_4553 PE=4 SV=1 |
304| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88P43_PSEPK | 21 | 6-phosphogluconate dehydratase OS=Pseudomonas putida (strain KT2440) GN=edd PE=4 SV=1 |
305| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | A0A379PS61_PSEPU | 19 | Heat shock protein Hsp20 OS=Pseudomonas putida OX=303 GN=ibpB PE=3 SV=1 |
306| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88KI8_PSEPK | 19 | Lon protease OS=Pseudomonas putida (strain KT2440) GN=lon-2 PE=2 SV=1 |
307| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88DS8_PSEPK | 19 | Type I restriction-modification system, M subunit OS=Pseudomonas putida (strain KT2440) GN=hsdM PE=4 SV=1 |
308| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88CL6_PSEPK | 18 | Penicillin amidase family protein OS=Pseudomonas putida (strain KT2440) GN=PP_5164 PE=4 SV=1 |
309| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88QP6_PSEPK | 18 | Transcription termination/antitermination protein NusG OS=Pseudomonas putida (strain KT2440) GN=nusG PE=3 SV=1 |
310| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88JT4_PSEPK | 17 | Antibiotic biosynthesis protein, putative OS=Pseudomonas putida (strain KT2440) GN=PP_2563 PE=4 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 310.1 | Q88JT4_PSEPK |
17 | 101542 | 10 (1) | 8 (1) | 0.02 | |
| Antibiotic biosynthesis protein, putative OS=Pseudomonas putida (strain KT2440) GN=PP_2563 PE=4 SV=1 | |||||||
10 peptide matches (10 non-duplicate, 0 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 261 |
559.3227 | 558.3154 | 558.3125 | 5.18 | 1 | 17 | 0.47 | 6Score > 26 indicates identity |
U | K.AIEAR.G | |
| 367 |
645.3320 | 644.3247 | 644.3354 | -16.6 | 1 | 10 | 0.64 | 4Score > 21 indicates identity |
U | E.ARGDAR.T | |
| 370 |
324.1667 | 646.3188 | 646.3286 | -15.1 | 0 | 8 | 1.9 | 9Score > 24 indicates identity |
U | R.ADSQVK.I | |
| 371 |
647.3278 | 646.3205 | 646.3286 | -12.5 | 0 | 10 | 1.4 | 7Score > 24 indicates identity |
U | R.ADSQVK.I | |
| 693 |
402.2176 | 802.4206 | 802.4337 | -16.3 | 0 | 3 | 6 | 9Score > 24 indicates identity |
U | E.ALQAFPR.Q + Deamidated (NQ) | |
| 1167 |
503.2969 | 1004.5792 | 1004.5767 | 2.55 | 1 | 4 | 1.1 | 1Score > 17 indicates identity |
U | E.ALIGHLPER.W | |
| 1399 |
374.5374 | 1120.5904 | 1120.5877 | 2.42 | 1 | 1 | 2.1 | 5Score > 20 indicates identity Score > 17 indicates homology |
U | R.FLGRADSQVK.I + Deamidated (NQ) | |
| 1551 |
591.3495 | 1180.6844 | 1180.6928 | -7.06 | 1 | 14 | 0.089 | 1Score > 16 indicates identity |
U | R.ILQVPRLDAR.L + Deamidated (NQ) | |
| 1552 |
1181.6923 | 1180.6850 | 1180.6928 | -6.57 | 1 | 17 | 0.045 | 1Score > 16 indicates identity |
U | R.ILQVPRLDAR.L + Deamidated (NQ) | |
| 2999 |
946.5106 | 1891.0066 | 1891.0203 | -7.23 | 1 | 2 | 2.9 | 2Score > 19 indicates identity |
U | E.ALQAFPRQPVALPAVSPE.Q + Deamidated (NQ) |
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