MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 12 Aug 2025 at 22:47:09 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 31–40 (out of 313)

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-31

Accession Score Description
Family member distances as a dendrogram 1 ATPG_PSEPK 335 ATP synthase gamma chain OS=Pseudomonas putida (strain KT2440) GN=atpG PE=3 SV=1
2 Q88MY8_PSEPK 23 description
Cut threshold

Score Mass Matches Sequences emPAI
ATPG_PSEPK 335 31558 14 (12) 11 (10) 1.18
ATP synthase gamma chain OS=Pseudomonas putida (strain KT2440) GN=atpG PE=3 SV=1
Q88MY8_PSEPK 23 0 3 (2) 3 (2) 0.09
description

-17 peptide matches (15 non-duplicate, 2 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U 1 2 Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U 1 2 Peptide
243   531.3516 530.3443 530.3428 2.92 0 29 0.0021 +1Score > 15 indicates identity U X K.IASIK.S
268   572.3781 571.3708 571.3694 2.57 0 25 0.013 +4Score > 19 indicates identity U X K.GGVVIK.E
526   364.7251 727.4356 727.4453 -13.3 1 6 1.3 +7Score > 19 indicates identity U X R.VLRQGR.A
566   373.7261 745.4376 745.4334 5.71 0 44 0.00011 +1Score > 17 indicates identity U X R.LSVVSNK.F
633   779.3958 778.3885 778.3895 -1.21 1 7 1.5 +4Score > 21 indicates identity U X K.ITSAMEK.V
702   407.2778 812.5410 812.5484 -9.01 1 14 0.04 +2Score > 13 indicates identity U X E.LKGGVVIK.E
1192   510.7921 1019.5696 1019.5651 4.45 1 32 0.0023 +1Score > 18 indicates identity U X K.ELQLIYNK.A
1245   523.2935 1044.5724 1044.5638 8.33 1 30 0.0037 +1Score > 21 indicates identity
Score > 18 indicates homology		 U X K.ELLDGLMVR.Y
1277   533.7819 1065.5492 1065.5455 3.54 0 61 3.6e-006 +1Score > 20 indicates identity U X R.AGYIVVSSDR.G
1426   566.2876 1130.5606 1130.5567 3.45 0 40 0.00093 +1Score > 22 indicates identity U X K.NATDNAGDLIK.E
1474   576.7941 1151.5736 1151.5645 7.96 0 64 1.7e-006 +1Score > 20 indicates identity
Score > 18 indicates homology		 U X K.VMLDAYLDGR.I
2333   525.2427 1572.7063 1572.6997 4.16 0 22 0.025 +1Score > 19 indicates identity U X K.HHWDYLYEPDAK.E
2359   791.4130 1580.8114 1580.8059 3.50 1 69 8.9e-007 +1Score > 21 indicates identity U X R.QVIGHLANANPEYR.H
2360 +1 527.9451 1580.8135 1580.8059 4.78 1 48 9.2e-005 +1Score > 21 indicates identity
Score > 20 indicates homology		 U X R.QVIGHLANANPEYR.H
4120 +1 913.1668 2736.4786 2736.4334 16.5 1 64 8.9e-007 +1Score > 16 indicates identity U X R.IFGGNVVAAISHLGEEPSINDLIGSVK.V + Deamidated (NQ)

3 subsets and intersections (4 subset proteins in total)

Score Mass Subset of
Q88MW9_PSEPK 38 158721 31.1, 31.2
Surface colonization protein, putative OS=Pseudomonas putida (strain KT2440) GN=PP_1449 PE=4 SV=1
Q88NT6_PSEPK 29 0 31.1
description
1 sameset of Q88NT6_PSEPK
Q88R05_PSEPK 29 0
description
Q88QF7_PSEPK 25 0 31.1, 31.2
description

+32

Accession Score Description
1 RPOA_PSEPK 333 DNA-directed RNA polymerase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=rpoA PE=3 SV=1

+33

Accession Score Description
1 RS3_PSEPK 311 30S ribosomal protein S3 OS=Pseudomonas putida (strain KT2440) GN=rpsC PE=3 SV=2

+34

Accession Score Description
1 Q88E12_PSEPK 307 Protocatechuate 3,4-dioxygenase, beta subunit OS=Pseudomonas putida (strain KT2440) GN=pcaH PE=4 SV=1

+35

Accession Score Description
1 Q88PS5_PSEPK 302 OmpA family protein OS=Pseudomonas putida (strain KT2440) GN=PP_0773 PE=3 SV=1

+36

Accession Score Description
1 RS16_PSEPK 299 30S ribosomal protein S16 OS=Pseudomonas putida (strain KT2440) GN=rpsP PE=3 SV=1

+37

Accession Score Description
1 TTGB_PSEPK 299 Probable efflux pump membrane transporter TtgB OS=Pseudomonas putida (strain KT2440) GN=ttgB PE=1 SV=1

+38

Accession Score Description
1 RL11_PSEPK 295 50S ribosomal protein L11 OS=Pseudomonas putida (strain KT2440) GN=rplK PE=3 SV=1

+39

Accession Score Description
1 RS4_PSEPK 295 30S ribosomal protein S4 OS=Pseudomonas putida (strain KT2440) GN=rpsD PE=3 SV=1

+40

Accession Score Description
1 TTGA_PSEPK 292 Probable efflux pump periplasmic linker TtgA OS=Pseudomonas putida (strain KT2440) GN=ttgA PE=1 SV=1
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