MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita1 |
| MS data file | : | PRT1270_T-BRSC_1_20250714114344.mgf |
| Database | : | P-putida 20180924 (5,556 sequences; 1,892,173 residues) |
| Timestamp | : | 12 Aug 2025 at 22:47:09 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,938 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
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301| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88CB4_PSEPK | 23 | Cytochrome c5 OS=Pseudomonas putida (strain KT2440) GN=PP_5267 PE=4 SV=1 |
302| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88QC1_PSEPK | 22 | Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_0573 PE=4 SV=1 |
303| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88EB3_PSEPK | 21 | RNA polymerase sigma-70 factor, ECF subfamily OS=Pseudomonas putida (strain KT2440) GN=PP_4553 PE=4 SV=1 |
304| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88P43_PSEPK | 21 | 6-phosphogluconate dehydratase OS=Pseudomonas putida (strain KT2440) GN=edd PE=4 SV=1 |
305| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | A0A379PS61_PSEPU | 19 | Heat shock protein Hsp20 OS=Pseudomonas putida OX=303 GN=ibpB PE=3 SV=1 |
306| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88KI8_PSEPK | 19 | Lon protease OS=Pseudomonas putida (strain KT2440) GN=lon-2 PE=2 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 306.1 | Q88KI8_PSEPK |
19 | 88895 | 9 (1) | 5 (1) | 0.03 | |
| Lon protease OS=Pseudomonas putida (strain KT2440) GN=lon-2 PE=2 SV=1 | |||||||
9 peptide matches (7 non-duplicate, 2 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 323 |
308.7061 | 615.3976 | 615.3955 | 3.45 | 1 | 8 | 0.55 | 4Score > 18 indicates identity |
U | E.LNKLK.Q + Deamidated (NQ) | |
| 357 |
637.3323 | 636.3250 | 636.3231 | 3.02 | 0 | 11 | 0.51 | 4Score > 20 indicates identity |
E.DALYR.V | ||
| 430 |
2 |
342.2265 | 682.4384 | 682.4378 | 1.01 | 0 | 19 | 0.016 | 1Score > 14 indicates identity |
U | E.AVVIPGK.G |
| 432 |
683.4470 | 682.4397 | 682.4378 | 2.88 | 0 | 9 | 0.16 | 2Score > 14 indicates identity |
U | E.AVVIPGK.G | |
| 446 |
344.7050 | 687.3954 | 687.4028 | -10.6 | 1 | 10 | 0.84 | 6Score > 22 indicates identity |
U | K.AQSKVR.L | |
| 692 |
803.4277 | 802.4204 | 802.4297 | -11.5 | 0 | 12 | 0.81 | 8Score > 24 indicates identity |
U | E.SIAAATNR.K | |
| 693 |
402.2176 | 802.4206 | 802.4297 | -11.3 | 0 | 2 | 8.1 | 10Score > 24 indicates identity |
U | E.SIAAATNR.K |
307| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88DS8_PSEPK | 19 | Type I restriction-modification system, M subunit OS=Pseudomonas putida (strain KT2440) GN=hsdM PE=4 SV=1 |
308| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88CL6_PSEPK | 18 | Penicillin amidase family protein OS=Pseudomonas putida (strain KT2440) GN=PP_5164 PE=4 SV=1 |
309| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88QP6_PSEPK | 18 | Transcription termination/antitermination protein NusG OS=Pseudomonas putida (strain KT2440) GN=nusG PE=3 SV=1 |
310| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88JT4_PSEPK | 17 | Antibiotic biosynthesis protein, putative OS=Pseudomonas putida (strain KT2440) GN=PP_2563 PE=4 SV=1 |
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