MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita1 |
| MS data file | : | PRT1270_T-BRSC_1_20250714114344.mgf |
| Database | : | P-putida 20180924 (5,556 sequences; 1,892,173 residues) |
| Timestamp | : | 12 Aug 2025 at 22:47:09 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,938 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
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281| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88J94_PSEPK | 26 | Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_2755 PE=4 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 281.1 | Q88J94_PSEPK |
26 | 45327 | 13 (1) | 4 (1) | 0.05 | |
| Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_2755 PE=4 SV=1 | |||||||
13 peptide matches (7 non-duplicate, 6 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 501 |
715.4481 | 714.4408 | 714.4500 | -12.9 | 1 | 14 | 0.34 | 6Score > 22 indicates identity |
U | E.TLRVAR.Q | |
| 502 |
358.2283 | 714.4420 | 714.4500 | -11.2 | 1 | 26 | 0.015 | 1Score > 20 indicates identity |
U | E.TLRVAR.Q | |
| 549 |
736.4011 | 735.3938 | 735.3915 | 3.13 | 0 | 20 | 0.072 | 3Score > 21 indicates identity |
U | K.VLQGYR.F + Deamidated (NQ) | |
| 645 |
393.7277 | 785.4408 | 785.4395 | 1.68 | 1 | 7 | 2.2 | 10Score > 23 indicates identity |
U | E.VAQRLAE.D | |
| 2190 |
6 |
752.3901 | 1502.7656 | 1502.7801 | -9.63 | 1 | 11 | 0.63 | 2Score > 21 indicates identity |
U | E.LGGTRSVSASQGLDR.F |
| 2192 |
501.9298 | 1502.7676 | 1502.7801 | -8.35 | 1 | 5 | 1.4 | 2Score > 21 indicates identity Score > 19 indicates homology |
U | E.LGGTRSVSASQGLDR.F | |
| 2206 |
502.2605 | 1503.7597 | 1503.7641 | -2.96 | 1 | 2 | 1.6 | 2Score > 21 indicates identity Score > 16 indicates homology |
U | E.LGGTRSVSASQGLDR.F + Deamidated (NQ) |
282| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL27_PSEPK | 26 | 50S ribosomal protein L27 OS=Pseudomonas putida (strain KT2440) GN=rpmA PE=3 SV=1 |
283| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88GL0_PSEPK | 26 | Sensory box protein OS=Pseudomonas putida (strain KT2440) GN=PP_3711 PE=4 SV=1 |
284| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RIMP_PSEPK | 26 | Ribosome maturation factor RimP OS=Pseudomonas putida (strain KT2440) GN=rimP PE=3 SV=2 |
285| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | NUOG_PSEPK | 26 | NADH-quinone oxidoreductase subunit G OS=Pseudomonas putida (strain KT2440) GN=nuoG PE=3 SV=1 |
286| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88E13_PSEPK | 26 | Protocatechuate 3,4-dioxygenase, alpha subunit OS=Pseudomonas putida (strain KT2440) GN=pcaG PE=4 SV=1 |
287| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88FG7_PSEPK | 26 | NADH dehydrogenase I, L subunit OS=Pseudomonas putida (strain KT2440) GN=nuoL PE=4 SV=1 |
288| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88Q73_PSEPK | 25 | Outer membrane protein assembly factor BamD OS=Pseudomonas putida (strain KT2440) GN=bamD PE=3 SV=1 |
289| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88H19_PSEPK | 24 | description |
290| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88GQ5_PSEPK | 24 | GGDEF domain protein OS=Pseudomonas putida (strain KT2440) GN=PP_3663 PE=4 SV=1 |
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