MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 12 Aug 2025 at 22:47:09 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 21–30 (out of 313)

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+21

Accession Score Description
1 RL2_PSEPK 571 50S ribosomal protein L2 OS=Pseudomonas putida (strain KT2440) GN=rplB PE=3 SV=1

+22

Accession Score Description
1 TTGC_PSEPK 498 Probable efflux pump outer membrane protein TtgC OS=Pseudomonas putida (strain KT2440) GN=ttgC PE=1 SV=1

+23

Accession Score Description
1 Q88EQ0_PSEPK 480 2-oxoisovalerate dehydrogenase, lipoamide acyltransferase component OS=Pseudomonas putida (strain KT2440) GN=bkdB PE=3 SV=1

+24

Accession Score Description
1 RL4_PSEPK 443 50S ribosomal protein L4 OS=Pseudomonas putida (strain KT2440) GN=rplD PE=3 SV=1

+25

Accession Score Description
1 RL5_PSEPK 416 50S ribosomal protein L5 OS=Pseudomonas putida (strain KT2440) GN=rplE PE=3 SV=1

+26

Accession Score Description
1 RL20_PSEPK 376 50S ribosomal protein L20 OS=Pseudomonas putida (strain KT2440) GN=rplT PE=3 SV=1

-27

Accession Score Description
1 SUCC_PSEPK 367 Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas putida (strain KT2440) GN=sucC PE=3 SV=1
Score Mass Matches Sequences emPAI
27.1 SUCC_PSEPK 367 41499 14 (13) 13 (12) 0.91
Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas putida (strain KT2440) GN=sucC PE=3 SV=1

-14 peptide matches (13 non-duplicate, 1 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
176   431.2636 430.2563 430.2540 5.44 0 37 0.00073 +1Score > 18 indicates identity U K.AGGVK.L
396   334.7272 667.4398 667.4381 2.60 0 31 0.00077 +1Score > 13 indicates identity U K.VPVVVR.L
460   347.6938 693.3730 693.3697 4.78 1 28 0.0075 +1Score > 19 indicates identity U R.VTEAFK.I
567   374.2431 746.4716 746.4691 3.47 0 34 0.0004 +1Score > 13 indicates identity U K.IFVGLAK.L
1127   496.2735 990.5324 990.5287 3.80 0 36 0.0016 +1Score > 21 indicates identity U K.AFAAQWLGK.N
1153   501.2767 1000.5388 1000.5342 4.67 1 30 0.007 +1Score > 23 indicates identity
Score > 21 indicates homology		 U K.IGGNEWVVK.A
1439   567.8148 1133.6150 1133.6081 6.16 1 14 0.21 +1Score > 20 indicates identity U K.ELYLGAVVDR.S
1762   430.1913 1287.5521 1287.5480 3.16 0 21 0.025 +1Score > 17 indicates identity U K.TFHDPSQDDAR.E
1870   676.3734 1350.7322 1350.7183 10.3 1 73 1.6e-007 +1Score > 18 indicates identity U K.QLFAEYGLPVSK.G
2329   785.4303 1568.8460 1568.8311 9.53 0 67 1e-006 +1Score > 20 indicates identity U K.ATIDPLVGAQPFQGR.E
2608   563.9954 1688.9644 1688.9349 17.5 1 36 0.00027 +1Score > 13 indicates identity U K.DHDLALLEVNPLVIK.A + Deamidated (NQ)
2720   868.4416 1734.8686 1734.8424 15.1 0 67 1.1e-006 +1Score > 20 indicates identity U K.NLVTYQTDANGQPVSK.I + Deamidated (NQ)
3842 +1 805.1207 2412.3403 2412.3111 12.1 1 65 3.4e-007 +1Score > 13 indicates identity U K.VLAESGLNIIAATSLTDAAQQVVK.A + Deamidated (NQ)

+28

Accession Score Description
1 Q88M03_PSEPK 353 30S ribosomal protein S1 OS=Pseudomonas putida (strain KT2440) GN=rpsA PE=3 SV=1

+29

Accession Score Description
1 RL16_PSEPK 348 50S ribosomal protein L16 OS=Pseudomonas putida (strain KT2440) GN=rplP PE=3 SV=1

+30

Accession Score Description
1 EFG1_PSEPK 340 Elongation factor G 1 OS=Pseudomonas putida (strain KT2440) GN=fusA PE=3 SV=1
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