MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 12 Aug 2025 at 22:47:09 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 21–30 (out of 313)

Page: Previous 1 2 3 4 5 6 7 8 … 32 Next 

+21

Accession Score Description
1 RL2_PSEPK 571 50S ribosomal protein L2 OS=Pseudomonas putida (strain KT2440) GN=rplB PE=3 SV=1

+22

Accession Score Description
1 TTGC_PSEPK 498 Probable efflux pump outer membrane protein TtgC OS=Pseudomonas putida (strain KT2440) GN=ttgC PE=1 SV=1

+23

Accession Score Description
1 Q88EQ0_PSEPK 480 2-oxoisovalerate dehydrogenase, lipoamide acyltransferase component OS=Pseudomonas putida (strain KT2440) GN=bkdB PE=3 SV=1

+24

Accession Score Description
1 RL4_PSEPK 443 50S ribosomal protein L4 OS=Pseudomonas putida (strain KT2440) GN=rplD PE=3 SV=1

+25

Accession Score Description
1 RL5_PSEPK 416 50S ribosomal protein L5 OS=Pseudomonas putida (strain KT2440) GN=rplE PE=3 SV=1

-26

Accession Score Description
1 RL20_PSEPK 376 50S ribosomal protein L20 OS=Pseudomonas putida (strain KT2440) GN=rplT PE=3 SV=1
Score Mass Matches Sequences emPAI
26.1 RL20_PSEPK 376 13272 23 (19) 12 (10) 9.04
50S ribosomal protein L20 OS=Pseudomonas putida (strain KT2440) GN=rplT PE=3 SV=1

-23 peptide matches (17 non-duplicate, 6 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
226   515.3321 514.3248 514.3227 4.08 0 5 4.1 +8Score > 26 indicates identity
Score > 23 indicates homology		 U R.GVIAR.K
260   558.3622 557.3549 557.3537 2.23 0 29 0.0073 +3Score > 20 indicates identity U K.QAVIK.A
318 +1 307.7162 613.4178 613.4163 2.56 0 36 0.00027 +1Score > 13 indicates identity U R.LIAGLK.K
405   336.2198 670.4250 670.4238 1.82 1 11 0.56 +3Score > 21 indicates identity U K.RGVIAR.K
436   343.6673 685.3200 685.3184 2.46 0 7 0.56 +1Score > 17 indicates identity U K.GYYGAR.S
437   686.3276 685.3203 685.3184 2.87 0 29 0.0035 +1Score > 17 indicates identity U K.GYYGAR.S
531   729.4426 728.4353 728.4333 2.75 0 22 0.043 +2Score > 20 indicates identity U R.ALWIAR.I
532   365.2252 728.4358 728.4333 3.47 0 25 0.02 +1Score > 20 indicates identity U R.ALWIAR.I
556   371.7640 741.5134 741.5112 2.99 1 38 0.00015 +1Score > 13 indicates identity U R.LIAGLKK.A
692   803.4277 802.4204 802.4185 2.45 1 39 0.0018 +1Score > 24 indicates identity U K.ASIEIDR.K
693   402.2176 802.4206 802.4185 2.73 1 25 0.038 +1Score > 24 indicates identity U K.ASIEIDR.K
731   414.7040 827.3934 827.3926 1.05 0 41 0.00024 +1Score > 18 indicates identity U K.AGQYAYR.D
732   828.4015 827.3942 827.3926 1.99 0 27 0.0049 +1Score > 17 indicates identity U K.AGQYAYR.D
905   449.7169 897.4192 897.4192 0.066 0 43 0.0002 +1Score > 19 indicates identity U R.TNGLSYSR.L + Deamidated (NQ)
954   919.5299 918.5226 918.5174 5.64 1 55 1e-005 +1Score > 18 indicates identity U K.AAFAAIVEK.A
955 +2 460.2702 918.5258 918.5174 9.15 1 44 0.00011 +1Score > 17 indicates identity U K.AAFAAIVEK.A
1290 +3 536.3088 1070.6030 1070.5972 5.51 1 65 2.3e-006 +1Score > 21 indicates identity U K.VLADLAVNEK.A

1 subset or intersection (1 subset protein in total)

Score Mass Subset of
A0A2N3Y5Z2_SACSN 49 275006 26.1
Rifamycin polyketide synthase module 1/2/3 OS=Saccharopolyspora spinosa OX=60894 GN=A8926_6432 PE=4 SV=1

+27

Accession Score Description
1 SUCC_PSEPK 367 Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas putida (strain KT2440) GN=sucC PE=3 SV=1

+28

Accession Score Description
1 Q88M03_PSEPK 353 30S ribosomal protein S1 OS=Pseudomonas putida (strain KT2440) GN=rpsA PE=3 SV=1

+29

Accession Score Description
1 RL16_PSEPK 348 50S ribosomal protein L16 OS=Pseudomonas putida (strain KT2440) GN=rplP PE=3 SV=1

+30

Accession Score Description
1 EFG1_PSEPK 340 Elongation factor G 1 OS=Pseudomonas putida (strain KT2440) GN=fusA PE=3 SV=1
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