MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 12 Aug 2025 at 22:47:09 GMT
Repeat search

[help]

Export

Not what you expected? Try the select summary.

Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

Filters

[help]

Show

Protein families 21–30 (out of 313)

Page: Previous 1 2 3 4 5 6 7 8 … 32 Next 

+21

Accession Score Description
1 RL2_PSEPK 571 50S ribosomal protein L2 OS=Pseudomonas putida (strain KT2440) GN=rplB PE=3 SV=1

+22

Accession Score Description
1 TTGC_PSEPK 498 Probable efflux pump outer membrane protein TtgC OS=Pseudomonas putida (strain KT2440) GN=ttgC PE=1 SV=1

+23

Accession Score Description
1 Q88EQ0_PSEPK 480 2-oxoisovalerate dehydrogenase, lipoamide acyltransferase component OS=Pseudomonas putida (strain KT2440) GN=bkdB PE=3 SV=1

+24

Accession Score Description
1 RL4_PSEPK 443 50S ribosomal protein L4 OS=Pseudomonas putida (strain KT2440) GN=rplD PE=3 SV=1

-25

Accession Score Description
1 RL5_PSEPK 416 50S ribosomal protein L5 OS=Pseudomonas putida (strain KT2440) GN=rplE PE=3 SV=1
Score Mass Matches Sequences emPAI
25.1 RL5_PSEPK 416 20316 23 (17) 12 (10) 2.32
50S ribosomal protein L5 OS=Pseudomonas putida (strain KT2440) GN=rplE PE=3 SV=1

-23 peptide matches (16 non-duplicate, 7 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
272   581.2704 580.2631 580.2605 4.47 0 19 0.055 +2Score > 19 indicates identity U K.SFDGR.G
331 +1 311.6825 621.3504 621.3486 2.97 0 18 0.021 +1Score > 13 indicates identity U K.SIAGFK.V
332   622.3580 621.3507 621.3486 3.42 0 10 0.12 +2Score > 13 indicates identity U K.SIAGFK.V
403   336.1896 670.3646 670.3650 -0.47 1 7 0.73 +2Score > 18 indicates identity U R.NEIAPK.L
404   671.3745 670.3672 670.3650 3.38 1 28 0.0052 +2Score > 18 indicates identity U R.NEIAPK.L
425   340.6805 679.3464 679.3442 3.32 1 17 0.1 +1Score > 19 indicates identity U K.FPFRN.-
462   348.1724 694.3302 694.3286 2.42 1 0 6 +9Score > 20 indicates identity U E.IYRNE.I + Deamidated (NQ)
573   375.7307 749.4468 749.4436 4.39 1 24 0.0041 +1Score > 13 indicates identity U R.KSIAGFK.V
865   882.5818 881.5745 881.5698 5.35 0 2 0.6 +1Score > 13 indicates identity U R.LLAISLPR.V
867 +3 441.7954 881.5762 881.5698 7.30 0 50 9e-006 +1Score > 13 indicates identity U R.LLAISLPR.V
935   304.8425 911.5057 911.5018 4.30 1 28 0.0062 +1Score > 19 indicates identity U R.AFKFPFR.N
1099   487.2276 972.4406 972.4375 3.25 1 45 0.00011 +1Score > 18 indicates identity U K.MYEFLDR.L
1510 +2 581.3317 1160.6488 1160.6401 7.53 0 52 2.5e-005 +1Score > 18 indicates identity U R.GLDITLTTTAR.S
1809   658.8898 1315.7650 1315.7612 2.90 0 53 8.8e-006 +1Score > 15 indicates identity U K.ITGQKPVVTFAR.K
1810 +1 439.5958 1315.7656 1315.7612 3.30 0 54 7.1e-006 +1Score > 15 indicates identity U K.ITGQKPVVTFAR.K
2052   716.3847 1430.7548 1430.7439 7.65 1 78 8.8e-008 +1Score > 20 indicates identity
Score > 20 indicates homology		 U K.ITLNMGLGEAIGDK.K

2 subsets and intersections (2 subset proteins in total)

Score Mass Subset of
Q88HM8_PSEPK 28 0 25.1
description
Q88IP4_PSEPK 19 0 25.1
description

+26

Accession Score Description
1 RL20_PSEPK 376 50S ribosomal protein L20 OS=Pseudomonas putida (strain KT2440) GN=rplT PE=3 SV=1

+27

Accession Score Description
1 SUCC_PSEPK 367 Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas putida (strain KT2440) GN=sucC PE=3 SV=1

+28

Accession Score Description
1 Q88M03_PSEPK 353 30S ribosomal protein S1 OS=Pseudomonas putida (strain KT2440) GN=rpsA PE=3 SV=1

+29

Accession Score Description
1 RL16_PSEPK 348 50S ribosomal protein L16 OS=Pseudomonas putida (strain KT2440) GN=rplP PE=3 SV=1

+30

Accession Score Description
1 EFG1_PSEPK 340 Elongation factor G 1 OS=Pseudomonas putida (strain KT2440) GN=fusA PE=3 SV=1
Page: Previous 1 2 3 4 5 6 7 8 … 32 Next 

Not what you expected? Try the select summary.

Mascot: http://www.matrixscience.com/