MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita1 |
| MS data file | : | PRT1270_T-BRSC_1_20250714114344.mgf |
| Database | : | P-putida 20180924 (5,556 sequences; 1,892,173 residues) |
| Timestamp | : | 12 Aug 2025 at 22:47:09 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,938 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle () in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
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21| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL2_PSEPK | 571 | 50S ribosomal protein L2 OS=Pseudomonas putida (strain KT2440) GN=rplB PE=3 SV=1 |
22| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | TTGC_PSEPK | 498 | Probable efflux pump outer membrane protein TtgC OS=Pseudomonas putida (strain KT2440) GN=ttgC PE=1 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 22.1 | TTGC_PSEPK |
498 | 52889 | 11 (10) | 10 (9) | 0.53 | |
| Probable efflux pump outer membrane protein TtgC OS=Pseudomonas putida (strain KT2440) GN=ttgC PE=1 SV=1 | |||||||
11 peptide matches (11 non-duplicate, 0 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
| 385 |
330.1960 | 658.3774 | 658.3650 | 18.9 | 0 | 10 | 1.4 | 5Score > 24 indicates identity Score > 24 indicates homology |
U | K.DINVAK.Y | |
| 978 |
464.7527 | 927.4908 | 927.4886 | 2.44 | 0 | 52 | 2.8e-005 | 1Score > 19 indicates identity |
U | K.AANANIGAAR.A | |
| 1941 |
692.8296 | 1383.6446 | 1383.6419 | 2.00 | 0 | 42 | 0.00031 | 1Score > 20 indicates identity |
U | R.DLVQANQDYYR.L | |
| 1969 |
697.8825 | 1393.7504 | 1393.7453 | 3.72 | 1 | 82 | 2.8e-008 | 1Score > 19 indicates identity |
U | R.LSQLTSEVNLYK.A | |
| 2053 |
716.8865 | 1431.7584 | 1431.7470 | 7.99 | 0 | 64 | 2.1e-006 | 1Score > 20 indicates identity |
U | R.NLFSAQQALIGDR.L | |
| 2057 |
718.3665 | 1434.7184 | 1434.7103 | 5.67 | 0 | 45 | 7.8e-005 | 1Score > 21 indicates identity Score > 16 indicates homology |
U | R.ADLFPAVSATGSGSR.Q | |
| 2314 |
781.9204 | 1561.8262 | 1561.8100 | 10.4 | 0 | 117 | 9.6e-012 | 1Score > 20 indicates identity |
U | R.IGIDSNLTFLDAQR.N | |
| 2395 |
801.9103 | 1601.8060 | 1601.8009 | 3.21 | 1 | 89 | 1.1e-008 | 1Score > 22 indicates identity |
U | R.SNEVGVASALDVSQAR.T | |
| 2643 |
569.6328 | 1705.8766 | 1705.8635 | 7.66 | 1 | 94 | 2.1e-009 | 1Score > 20 indicates identity |
U | K.TIQTAFQEVSDGLAAR.K | |
| 2644 |
854.4401 | 1706.8656 | 1706.8475 | 10.6 | 1 | 51 | 4.7e-005 | 1Score > 20 indicates identity |
U | K.TIQTAFQEVSDGLAAR.K + Deamidated (NQ) | |
| 3789 |
790.4212 | 2368.2418 | 2368.2288 | 5.49 | 0 | 36 | 0.0008 | 1Score > 18 indicates identity |
U | R.QFFHDPALQQLIQTSLVNNR.D |
23| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88EQ0_PSEPK | 480 | 2-oxoisovalerate dehydrogenase, lipoamide acyltransferase component OS=Pseudomonas putida (strain KT2440) GN=bkdB PE=3 SV=1 |
24| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL4_PSEPK | 443 | 50S ribosomal protein L4 OS=Pseudomonas putida (strain KT2440) GN=rplD PE=3 SV=1 |
25| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL5_PSEPK | 416 | 50S ribosomal protein L5 OS=Pseudomonas putida (strain KT2440) GN=rplE PE=3 SV=1 |
26| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL20_PSEPK | 376 | 50S ribosomal protein L20 OS=Pseudomonas putida (strain KT2440) GN=rplT PE=3 SV=1 |
27| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | SUCC_PSEPK | 367 | Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas putida (strain KT2440) GN=sucC PE=3 SV=1 |
28| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88M03_PSEPK | 353 | 30S ribosomal protein S1 OS=Pseudomonas putida (strain KT2440) GN=rpsA PE=3 SV=1 |
29| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL16_PSEPK | 348 | 50S ribosomal protein L16 OS=Pseudomonas putida (strain KT2440) GN=rplP PE=3 SV=1 |
30| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | EFG1_PSEPK | 340 | Elongation factor G 1 OS=Pseudomonas putida (strain KT2440) GN=fusA PE=3 SV=1 |
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