MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita1 |
| MS data file | : | PRT1270_T-BRSC_1_20250714114344.mgf |
| Database | : | P-putida 20180924 (5,556 sequences; 1,892,173 residues) |
| Timestamp | : | 12 Aug 2025 at 22:47:09 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,938 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
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211| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RS12_PSEPK | 48 | 30S ribosomal protein S12 OS=Pseudomonas putida (strain KT2440) GN=rpsL PE=3 SV=1 |
212| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88EH7_PSEPK | 47 | Basic amino acid ABC transporter, periplasmic basic amino acid-binding protein OS=Pseudomonas putida (strain KT2440) GN=PP_4486 PE=4 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 212.1 | Q88EH7_PSEPK |
47 | 28399 | 4 (1) | 3 (1) | 0.08 | |
| Basic amino acid ABC transporter, periplasmic basic amino acid-binding protein OS=Pseudomonas putida (strain KT2440) GN=PP_4486 PE=4 SV=1 | |||||||
4 peptide matches (4 non-duplicate, 0 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 1061 |
478.7827 | 955.5508 | 955.5450 | 6.07 | 0 | 6 | 1.1 | 2Score > 19 indicates identity |
U | R.INAAIDAIR.A | |
| 1066 |
479.2728 | 956.5310 | 956.5291 | 2.09 | 0 | 14 | 0.26 | 3Score > 21 indicates identity |
U | R.INAAIDAIR.A + Deamidated (NQ) | |
| 2032 |
712.3901 | 1422.7656 | 1422.7547 | 7.68 | 1 | 12 | 0.28 | 1Score > 19 indicates identity |
U | K.IGIEAAYPPFAFK.Q | |
| 2803 |
889.4735 | 1776.9324 | 1776.9146 | 10.1 | 1 | 47 | 0.0001 | 1Score > 19 indicates identity |
U | R.LDGTVADATLLEDGFLK.T |
213| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88DN0_PSEPK | 46 | LPS-assembly lipoprotein LptE OS=Pseudomonas putida (strain KT2440) GN=lptE PE=3 SV=1 |
214| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL9_PSEPK | 46 | 50S ribosomal protein L9 OS=Pseudomonas putida (strain KT2440) GN=rplI PE=3 SV=1 |
215| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88KS2_PSEPK | 45 | Enoyl-CoA hydratase/isomerase FadB1x OS=Pseudomonas putida (strain KT2440) GN=fadB1x PE=3 SV=1 |
216| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88NY3_PSEPK | 44 | Amino acid ABC transporter, permease protein OS=Pseudomonas putida (strain KT2440) GN=PP_1070 PE=3 SV=1 |
217| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | DAPD_PSEPK | 44 | 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase OS=Pseudomonas putida (strain KT2440) GN=dapD PE=3 SV=1 |
218| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88CG5_PSEPK | 42 | Exopolyphosphatase OS=Pseudomonas putida (strain KT2440) GN=ppx PE=4 SV=1 |
219| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | AK_PSEPK | 42 | Aspartate kinase OS=Pseudomonas putida (strain KT2440) GN=PP_4473 PE=1 SV=1 |
220| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88F49_PSEPK | 42 | Cytochrome c oxidase, cbb3-type, subunit I OS=Pseudomonas putida (strain KT2440) GN=ccoN-1 PE=3 SV=1 |
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