MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 12 Aug 2025 at 22:47:09 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 21–30 (out of 313)

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-21

Accession Score Description
1 RL2_PSEPK 571 50S ribosomal protein L2 OS=Pseudomonas putida (strain KT2440) GN=rplB PE=3 SV=1
Score Mass Matches Sequences emPAI
21.1 RL2_PSEPK 571 29822 24 (20) 13 (12) 2.30
50S ribosomal protein L2 OS=Pseudomonas putida (strain KT2440) GN=rplB PE=3 SV=1

-24 peptide matches (19 non-duplicate, 5 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
321   615.3600 614.3527 614.3500 4.45 0 27 0.014 +2Score > 21 indicates identity U K.GAQIAR.S
372   325.1879 648.3612 648.3595 2.68 0 20 0.029 +1Score > 18 indicates identity U R.LVDFR.R
484   352.7217 703.4288 703.4268 2.86 0 30 0.0011 +1Score > 13 indicates identity U R.YIIAPK.G
485   704.4369 703.4296 703.4268 3.97 0 40 9.1e-005 +1Score > 13 indicates identity U R.YIIAPK.G
643   392.7442 783.4738 783.4715 2.96 0 26 0.0046 +1Score > 15 indicates identity U R.GVRPTVR.G
818   429.7447 857.4748 857.4719 3.45 0 44 0.0004 +1Score > 23 indicates identity U K.AGNSLQLR.N
925   453.7201 905.4256 905.4243 1.52 1 39 0.0011 +1Score > 22 indicates identity U R.IEYDPNR.T
996   468.7626 935.5106 935.5076 3.23 1 20 0.036 +1Score > 18 indicates identity U R.EGVYVTLR.L
1213 +1 344.8671 1031.5795 1031.5764 3.02 1 34 0.0013 +1Score > 18 indicates identity U K.GAPHAPLIEK.K
1214   516.7978 1031.5810 1031.5764 4.55 1 38 0.00062 +1Score > 18 indicates identity U K.GAPHAPLIEK.K
1243 +1 522.7986 1043.5826 1043.5723 9.89 0 54 2.1e-005 +1Score > 20 indicates identity U R.SAGASAQLIAR.E
1665   617.3167 1232.6188 1232.6414 -18.3 1 0 3.9 +5Score > 22 indicates identity
Score > 19 indicates homology		 U K.QHYRLVDFR.R
1698 +1 626.8296 1251.6446 1251.6401 3.67 0 66 7e-007 +1Score > 20 indicates identity
Score > 17 indicates homology		 U R.HPVSPWGFPTK.G
2354 +2 790.9595 1579.9044 1579.8933 7.03 0 91 1.3e-009 +1Score > 14 indicates identity U K.GVSAGDQLIAGALAPIK.A
2355   527.6426 1579.9060 1579.8933 7.99 0 61 1.3e-006 +1Score > 14 indicates identity U K.GVSAGDQLIAGALAPIK.A
2753   587.3401 1758.9985 1758.9992 -0.42 1 23 0.0069 +1Score > 14 indicates identity U R.NIPVGSTIHGIELKPGK.G
2754   440.7589 1759.0065 1758.9992 4.14 1 13 0.066 +1Score > 13 indicates identity U R.NIPVGSTIHGIELKPGK.G
2756   441.0092 1760.0077 1759.9832 13.9 1 14 0.042 +1Score > 13 indicates identity U R.NIPVGSTIHGIELKPGK.G + Deamidated (NQ)
2757   587.6773 1760.0101 1759.9832 15.3 1 10 0.11 +1Score > 13 indicates identity U R.NIPVGSTIHGIELKPGK.G + Deamidated (NQ)

1 subset or intersection (1 subset protein in total)

Score Mass Subset of
Q88DZ5_PSEPK 27 0 21.1
description

+22

Accession Score Description
1 TTGC_PSEPK 498 Probable efflux pump outer membrane protein TtgC OS=Pseudomonas putida (strain KT2440) GN=ttgC PE=1 SV=1

+23

Accession Score Description
1 Q88EQ0_PSEPK 480 2-oxoisovalerate dehydrogenase, lipoamide acyltransferase component OS=Pseudomonas putida (strain KT2440) GN=bkdB PE=3 SV=1

+24

Accession Score Description
1 RL4_PSEPK 443 50S ribosomal protein L4 OS=Pseudomonas putida (strain KT2440) GN=rplD PE=3 SV=1

+25

Accession Score Description
1 RL5_PSEPK 416 50S ribosomal protein L5 OS=Pseudomonas putida (strain KT2440) GN=rplE PE=3 SV=1

+26

Accession Score Description
1 RL20_PSEPK 376 50S ribosomal protein L20 OS=Pseudomonas putida (strain KT2440) GN=rplT PE=3 SV=1

+27

Accession Score Description
1 SUCC_PSEPK 367 Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas putida (strain KT2440) GN=sucC PE=3 SV=1

+28

Accession Score Description
1 Q88M03_PSEPK 353 30S ribosomal protein S1 OS=Pseudomonas putida (strain KT2440) GN=rpsA PE=3 SV=1

+29

Accession Score Description
1 RL16_PSEPK 348 50S ribosomal protein L16 OS=Pseudomonas putida (strain KT2440) GN=rplP PE=3 SV=1

+30

Accession Score Description
1 EFG1_PSEPK 340 Elongation factor G 1 OS=Pseudomonas putida (strain KT2440) GN=fusA PE=3 SV=1
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