MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita1 |
| MS data file | : | PRT1270_T-BRSC_1_20250714114344.mgf |
| Database | : | P-putida 20180924 (5,556 sequences; 1,892,173 residues) |
| Timestamp | : | 12 Aug 2025 at 22:47:09 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,938 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
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201| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88MY6_PSEPK | 56 | Signal peptidase I OS=Pseudomonas putida (strain KT2440) GN=lepB PE=3 SV=1 |
202| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ANT3_HUMAN | 56 | Antithrombin-III OS=Homo sapiens GN=SERPINC1 PE=1 SV=1 |
203| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88P29_PSEPK | 53 | 2-dehydro-3-deoxyphosphogluconate aldolase/4-hydroxy-2-oxoglutarate aldolase OS=Pseudomonas putida (strain KT2440) GN=eda PE=4 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 203.1 | Q88P29_PSEPK |
53 | 25273 | 6 (3) | 4 (3) | 0.30 | |
| 2-dehydro-3-deoxyphosphogluconate aldolase/4-hydroxy-2-oxoglutarate aldolase OS=Pseudomonas putida (strain KT2440) GN=eda PE=4 SV=1 | |||||||
6 peptide matches (4 non-duplicate, 2 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 467 |
350.7220 | 699.4294 | 699.4279 | 2.21 | 0 | 1 | 7.2 | 10Score > 22 indicates identity |
U | K.AIQVLR.E + Deamidated (NQ) | |
| 744 |
2 |
416.2511 | 830.4876 | 830.4862 | 1.79 | 1 | 31 | 0.0027 | 1Score > 18 indicates identity |
U | R.TLEVTLR.S |
| 1137 |
498.3380 | 994.6614 | 994.6539 | 7.60 | 0 | 24 | 0.0045 | 1Score > 13 indicates identity |
U | R.ILPVITIAR.E | |
| 1229 |
518.7681 | 1035.5216 | 1035.5138 | 7.58 | 0 | 28 | 0.0022 | 1Score > 21 indicates identity Score > 14 indicates homology |
U | K.AFGGPFGDIR.F |
204| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88P55_PSEPK | 51 | Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_0998 PE=4 SV=1 |
205| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88P30_PSEPK | 51 | 6-phosphogluconolactonase OS=Pseudomonas putida (strain KT2440) GN=pgl PE=4 SV=1 |
206| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RS8_PSEPK | 51 | 30S ribosomal protein S8 OS=Pseudomonas putida (strain KT2440) GN=rpsH PE=3 SV=1 |
207| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ATPD_PSEPK | 50 | ATP synthase subunit delta OS=Pseudomonas putida (strain KT2440) GN=atpH PE=3 SV=1 |
208| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | PAL_PSEPK | 49 | Peptidoglycan-associated lipoprotein OS=Pseudomonas putida (strain KT2440) GN=pal PE=3 SV=1 |
209| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88HC7_PSEPK | 49 | 4-hydroxyphenylpyruvate dioxygenase OS=Pseudomonas putida (strain KT2440) GN=hpd PE=3 SV=1 |
210| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88NX1_PSEPK | 49 | Ferroxidase OS=Pseudomonas putida (strain KT2440) GN=bfr PE=3 SV=1 |
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