MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 12 Aug 2025 at 22:47:09 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 11–20 (out of 313)

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+11

Accession Score Description
Family member distances as a dendrogram 1 Q88NK1_PSEPK 752 Porin D OS=Pseudomonas putida (strain KT2440) GN=oprD PE=4 SV=1
3 Q88MB8_PSEPK 45 GTP pyrophosphokinase OS=Pseudomonas putida (strain KT2440) GN=relA PE=3 SV=1
2 Q88R66_PSEPK 273 Outer membrane protein OprE3 OS=Pseudomonas putida (strain KT2440) GN=oprQ PE=4 SV=1

+12

Accession Score Description
1 Q88FA9_PSEPK 748 2-oxoglutarate dehydrogenase, E1 component OS=Pseudomonas putida (strain KT2440) GN=kgdA PE=4 SV=1

+13

Accession Score Description
1 RPOC_PSEPK 704 DNA-directed RNA polymerase subunit beta' OS=Pseudomonas putida (strain KT2440) GN=rpoC PE=3 SV=1

+14

Accession Score Description
1 RL3_PSEPK 676 50S ribosomal protein L3 OS=Pseudomonas putida (strain KT2440) GN=rplC PE=3 SV=2

+15

Accession Score Description
1 Q88FB1_PSEPK 674 Dihydrolipoyl dehydrogenase OS=Pseudomonas putida (strain KT2440) GN=lpdG PE=4 SV=1

+16

Accession Score Description
1 RS2_PSEPK 613 30S ribosomal protein S2 OS=Pseudomonas putida (strain KT2440) GN=rpsB PE=3 SV=1

+17

Accession Score Description
1 RS5_PSEPK 611 30S ribosomal protein S5 OS=Pseudomonas putida (strain KT2440) GN=rpsE PE=3 SV=1

+18

Accession Score Description
1 Q88R99_PSEPK 608 Porin E OS=Pseudomonas putida (strain KT2440) GN=oprE PE=4 SV=1

+19

Accession Score Description
1 CH60_PSEPK 589 60 kDa chaperonin OS=Pseudomonas putida (strain KT2440) GN=groL PE=3 SV=1

-20

Accession Score Description
1 Q88PD7_PSEPK 581 Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_0913 PE=4 SV=1
Score Mass Matches Sequences emPAI
20.1 Q88PD7_PSEPK 581 47104 20 (18) 14 (13) 1.14
Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_0913 PE=4 SV=1

-20 peptide matches (17 non-duplicate, 3 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
202   474.2695 473.2622 473.2598 5.16 0 1 3.1 +5Score > 29 indicates identity
Score > 18 indicates homology		 E.QAAGK.L
680   400.7484 799.4822 799.4803 2.40 0 29 0.0099 +1Score > 22 indicates identity U R.DAIAALVK.Q
681   800.4903 799.4830 799.4803 3.37 0 62 3.6e-006 +1Score > 20 indicates identity U R.DAIAALVK.Q
787   425.7160 849.4174 849.4133 4.87 1 38 0.0011 +1Score > 21 indicates identity U K.EAWFAAR.V
1090   485.7657 969.5168 969.5131 3.87 0 36 0.0008 +1Score > 20 indicates identity
Score > 17 indicates homology		 U K.VDPAADAALK.A
1332   545.2818 1088.5490 1088.5462 2.63 1 82 5.6e-008 +1Score > 22 indicates identity U K.QTGSIEQAAGK.L
1441   379.2342 1134.6808 1134.6761 4.13 0 15 0.03 +1Score > 13 indicates identity U K.LQVIVDHALK.G
1531 +2 586.8517 1171.6888 1171.6812 6.50 0 51 1.5e-005 +1Score > 15 indicates identity U K.LTGPSLSSLVAK.V
1608   606.8166 1211.6186 1211.6146 3.35 1 46 0.00017 +1Score > 21 indicates identity U K.LEAEPVNDGLR.K
1683   620.3246 1238.6346 1238.6295 4.13 1 30 0.0019 +1Score > 20 indicates identity
Score > 15 indicates homology		 U R.VPYLQSETFR.F
2420   806.9015 1611.7884 1611.7893 -0.51 1 48 3.5e-005 +1Score > 21 indicates identity
Score > 16 indicates homology		 U R.NVYLGEYTRPDGTK.L
2421   538.2713 1611.7921 1611.7893 1.73 1 30 0.007 +1Score > 21 indicates identity U R.NVYLGEYTRPDGTK.L
2458   813.8967 1625.7788 1625.7685 6.34 1 45 0.00016 +1Score > 20 indicates identity U K.LGIANLNPDTADHEF.-
3289 +1 692.7125 2075.1157 2075.0786 17.9 1 61 2.1e-006 +1Score > 17 indicates identity U K.TLQTAVDAFLAKPNDETLK.A + Deamidated (NQ)
3423   714.6862 2141.0368 2141.0137 10.8 1 66 1.5e-006 +1Score > 20 indicates identity U K.GEHYDQLIAADNAAGNQVVR.D + Deamidated (NQ)
3651   1139.5818 2277.1490 2277.1237 11.2 1 92 2.6e-009 +1Score > 19 indicates identity U K.AAAPQAQAPAAATTAAPGAVDEAAGK.A + Deamidated (NQ)
3652   760.0572 2277.1498 2277.1237 11.5 1 65 1.4e-006 +1Score > 19 indicates identity U K.AAAPQAQAPAAATTAAPGAVDEAAGK.A + Deamidated (NQ)
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