MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 12 Aug 2025 at 22:47:09 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 1–10 (out of 313)

Page: 1 2 3 4 5 6 … 32 Next 

+1

Accession Score Description
1 Q88NM2_PSEPK 4307 Outer membrane protein H1 OS=Pseudomonas putida (strain KT2440) GN=oprH PE=4 SV=1

-2

Accession Score Description
Family member distances as a dendrogram 1 EFTU2_PSEPK 2609 Elongation factor Tu-B OS=Pseudomonas putida (strain KT2440) GN=tufB PE=3 SV=1
2 EFTU1_PSEPK 2412 Elongation factor Tu-A OS=Pseudomonas putida (strain KT2440) GN=tufA PE=1 SV=1
Cut threshold

Score Mass Matches Sequences emPAI
EFTU2_PSEPK 2609 43793 98 (92) 18 (17) 7.97
Elongation factor Tu-B OS=Pseudomonas putida (strain KT2440) GN=tufB PE=3 SV=1
EFTU1_PSEPK 2412 43810 92 (86) 18 (17) 7.53
Elongation factor Tu-A OS=Pseudomonas putida (strain KT2440) GN=tufA PE=1 SV=1

-104 peptide matches (48 non-duplicate, 56 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U 1 2 Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U 1 2 Peptide
448 +1 345.2011 688.3876 688.3868 1.22 0 32 0.0026 +1Score > 21 indicates identity
Score > 18 indicates homology		 U X X R.GTVVTGR.I
451 +1 689.3964 688.3891 688.3868 3.36 0 28 0.011 +1Score > 21 indicates identity U X X R.GTVVTGR.I
471   702.3801 701.3728 701.3708 2.90 1 35 0.0053 +1Score > 25 indicates identity U X X K.LLDEGR.A
472 +1 351.6937 701.3728 701.3708 2.94 1 27 0.036 +1Score > 25 indicates identity U X X K.LLDEGR.A
621 +3 388.7091 775.4036 775.4017 2.49 0 24 0.031 +1Score > 21 indicates identity U X X R.HTPFFK.G
624   776.4114 775.4041 775.4017 3.10 0 32 0.0025 +1Score > 21 indicates identity
Score > 18 indicates homology		 U X X R.HTPFFK.G
686 +2 401.2473 800.4800 800.4756 5.54 0 44 0.00029 +1Score > 21 indicates identity U X X R.TVGAGVVAK.I
687 +1 801.4874 800.4801 800.4756 5.63 0 51 5.2e-005 +1Score > 21 indicates identity U X X R.TVGAGVVAK.I
834   867.5073 866.5000 866.4974 3.06 1 54 9.1e-006 +1Score > 16 indicates identity U X X R.EHILLSR.Q
835 +2 434.2573 866.5000 866.4974 3.08 1 44 0.0001 +1Score > 16 indicates identity U X X R.EHILLSR.Q
1038 +1 947.5552 946.5479 946.5447 3.36 0 51 2.2e-005 +1Score > 17 indicates identity U X X K.TTLTAALTR.V
1039 +2 474.2820 946.5494 946.5447 4.97 0 81 2.3e-008 +1Score > 17 indicates identity U X X K.TTLTAALTR.V
1164 +1 503.2565 1004.4984 1004.4961 2.37 1 47 0.00018 +1Score > 22 indicates identity U X X K.TIAMEDGLR.F
1193   511.2544 1020.4942 1020.4910 3.20 1 39 0.00095 +1Score > 21 indicates identity U X X K.TIAMEDGLR.F + Oxidation (M)
1331   544.7812 1087.5478 1087.5444 3.15 1 101 6.4e-010 +1Score > 22 indicates identity U X X R.AGENCGVLLR.G
1480 +1 1156.6298 1155.6225 1155.6176 4.29 1 67 1.2e-006 +1Score > 20 indicates identity U X X K.FTAEVYVLSK.E
1482 +8 578.8196 1155.6246 1155.6176 6.13 1 49 6.1e-005 +1Score > 20 indicates identity
Score > 19 indicates homology		 U X X K.FTAEVYVLSK.E
1660 +2 617.3128 1232.6110 1232.6091 1.60 0 28 0.0091 +1Score > 21 indicates identity
Score > 21 indicates homology		 U X X K.GYRPQFYFR.T
1663 +3 411.8792 1232.6158 1232.6091 5.44 0 33 0.0044 +1Score > 22 indicates identity U X X K.GYRPQFYFR.T
1675   413.5777 1237.7113 1237.7030 6.66 1 61 1.6e-006 +1Score > 16 indicates identity U X X R.VQDPLEIVGLR.D
1676 +4 619.8635 1237.7124 1237.7030 7.61 1 69 2.9e-007 +1Score > 16 indicates identity U X X R.VQDPLEIVGLR.D
2120   492.6282 1474.8628 1474.8548 5.41 0 41 7.6e-005 +1Score > 13 indicates identity U X X R.QVGVPYIVVFLNK.A
2123 +3 738.4399 1474.8652 1474.8548 7.09 0 69 1.3e-007 +1Score > 13 indicates identity U X X R.QVGVPYIVVFLNK.A
2342 +2 315.7942 1573.9346 1573.9304 2.67 0 25 0.0032 +1Score > 13 indicates identity U X X R.GQVLVKPGSVKPHTK.F
2343   787.9747 1573.9348 1573.9304 2.81 0 72 7e-008 +1Score > 13 indicates identity U X X R.GQVLVKPGSVKPHTK.F
2344 +1 394.4910 1573.9349 1573.9304 2.84 0 37 0.00022 +1Score > 13 indicates identity U X X R.GQVLVKPGSVKPHTK.F
2345 +1 525.6525 1573.9357 1573.9304 3.34 0 57 2.1e-006 +1Score > 13 indicates identity U X X R.GQVLVKPGSVKPHTK.F
2440 +4 807.9468 1613.8790 1613.8665 7.80 1 80 4.4e-008 +1Score > 19 indicates identity U X K.LVETLDAYIPEPVR.A
2441 +1 538.9677 1613.8813 1613.8665 9.18 1 38 0.00068 +1Score > 19 indicates identity U X K.LVETLDAYIPEPVR.A
2463 +2 815.9435 1629.8724 1629.8614 6.79 1 92 3e-009 +1Score > 20 indicates identity U X K.LVETLDSYIPEPVR.A
2465   544.3011 1629.8815 1629.8614 12.3 1 32 0.003 +1Score > 19 indicates identity U X K.LVETLDSYIPEPVR.A
2772   883.9711 1765.9276 1765.9224 2.98 0 83 2e-008 +1Score > 18 indicates identity U X X R.SLPHVNVGTIGHVDHGK.T
2773 +2 354.1929 1765.9281 1765.9224 3.24 0 27 0.008 +1Score > 18 indicates identity U X X R.SLPHVNVGTIGHVDHGK.T
2776 +1 442.4898 1765.9301 1765.9224 4.36 0 48 5.5e-005 +1Score > 18 indicates identity U X X R.SLPHVNVGTIGHVDHGK.T
2777   589.6522 1765.9348 1765.9224 7.01 0 44 0.00015 +1Score > 18 indicates identity U X X R.SLPHVNVGTIGHVDHGK.T
D:\Xcalibur\Data\Jennifer\PRT1270 Rita DDA\PRT1270_T-BRSC_1_20250714114344.raw

Score > 19 indicates identity
2779 +2 442.7401 1766.9313 1766.9064 14.1 0 44 0.00017 -1Score > 19 indicates identity U X X R.SLPHVNVGTIGHVDHGK.T + Deamidated (NQ)
No other peptide matches in query
2780   589.9844 1766.9314 1766.9064 14.1 0 55 1.1e-005 +1Score > 19 indicates identity U X X R.SLPHVNVGTIGHVDHGK.T + Deamidated (NQ)
2781   354.3936 1766.9316 1766.9064 14.3 0 28 0.0062 +1Score > 19 indicates identity U X X R.SLPHVNVGTIGHVDHGK.T + Deamidated (NQ)
2783   442.9528 1767.7821 1767.7787 1.89 0 8 0.67 +1Score > 18 indicates identity U X X R.HYAHVDCPGHADYVK.N
2815   894.9700 1787.9254 1787.9166 4.95 1 86 1.7e-008 +1Score > 20 indicates identity U X R.GITINTAHVEYNSTIR.H
2820   597.3161 1788.9265 1788.9006 14.5 1 47 0.00012 +1Score > 21 indicates identity U X R.GITINTAHVEYNSTIR.H + Deamidated (NQ)
2839   601.3135 1800.9187 1800.9118 3.80 1 67 1.1e-006 +1Score > 20 indicates identity U X R.GITINTAHVEYNSNIR.H
2840 +1 901.4675 1800.9204 1800.9118 4.78 1 106 1.5e-010 +1Score > 20 indicates identity
Score > 20 indicates homology		 U X R.GITINTAHVEYNSNIR.H
2846 +1 601.6489 1801.9249 1801.8958 16.1 1 50 3.7e-005 +1Score > 20 indicates identity
Score > 19 indicates homology		 U X R.GITINTAHVEYNSNIR.H + Deamidated (NQ)
3408   712.3768 2134.1086 2134.0769 14.9 1 83 1.9e-008 +1Score > 19 indicates identity U X X R.AIDQPFLMPIEDVFSISGR.G
3410 +1 1068.5546 2135.0946 2135.0609 15.8 1 88 6.9e-009 +1Score > 19 indicates identity U X X R.AIDQPFLMPIEDVFSISGR.G + Deamidated (NQ)
3412   712.7077 2135.1013 2135.0609 18.9 1 83 2.2e-008 +1Score > 19 indicates identity U X X R.AIDQPFLMPIEDVFSISGR.G + Deamidated (NQ)
3451 +1 1076.5529 2151.0912 2151.0558 16.5 1 77 1.1e-007 +1Score > 20 indicates identity U X X R.AIDQPFLMPIEDVFSISGR.G + Deamidated (NQ); Oxidation (M)

1 subset or intersection (2 subset proteins in total)

Score Mass Subset of
GLNE_PSEPK 37 109651 2.1
Glutamate-ammonia-ligase adenylyltransferase OS=Pseudomonas putida (strain KT2440) GN=glnE PE=3 SV=1
1 sameset of GLNE_PSEPK
RAPA_PSEPK 37 106215
RNA polymerase-associated protein RapA OS=Pseudomonas putida (strain KT2440) GN=rapA PE=3 SV=1

+3

Accession Score Description
1 ATPB_PSEPK 2344 ATP synthase subunit beta OS=Pseudomonas putida (strain KT2440) GN=atpD PE=3 SV=1

+4

Accession Score Description
1 Q88ES5_PSEPK 2322 Flagellin OS=Pseudomonas putida (strain KT2440) GN=fliC PE=3 SV=1

+5

Accession Score Description
1 Q88L46_PSEPK 1745 Outer membrane protein OprF OS=Pseudomonas putida (strain KT2440) GN=oprF PE=3 SV=1

+6

Accession Score Description
1 ATPA_PSEPK 1711 ATP synthase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=atpA PE=3 SV=1

+7

Accession Score Description
1 Q88FB0_PSEPK 1295 Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex OS=Pseudomonas putida (strain KT2440) GN=kgdB PE=3 SV=1

+8

Accession Score Description
1 Q88FA7_PSEPK 1233 Succinate dehydrogenase flavoprotein subunit OS=Pseudomonas putida (strain KT2440) GN=sdhA PE=3 SV=1

+9

Accession Score Description
1 RPOB_PSEPK 843 DNA-directed RNA polymerase subunit beta OS=Pseudomonas putida (strain KT2440) GN=rpoB PE=3 SV=1

+10

Accession Score Description
1 Q88FA8_PSEPK 775 Succinate dehydrogenase, iron-sulfur protein OS=Pseudomonas putida (strain KT2440) GN=sdhB PE=4 SV=1
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