MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita1 |
| MS data file | : | PRT1270_T-BRSC_1_20250714114344.mgf |
| Database | : | P-putida 20180924 (5,556 sequences; 1,892,173 residues) |
| Timestamp | : | 12 Aug 2025 at 22:47:09 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,938 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
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191| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | CLPX_PSEPK | 60 | ATP-dependent Clp protease ATP-binding subunit ClpX OS=Pseudomonas putida (strain KT2440) GN=clpX PE=3 SV=1 |
192| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88RK3_PSEPK | 60 | Cytochrome c4 OS=Pseudomonas putida (strain KT2440) GN=PP_0126 PE=4 SV=1 |
193| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88K29_PSEPK | 59 | Cold shock protein CspA OS=Pseudomonas putida (strain KT2440) GN=cspA-2 PE=4 SV=1 |
194| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88Q14_PSEPK | 59 | Peptidyl-prolyl cis-trans isomerase OS=Pseudomonas putida (strain KT2440) GN=fklB-1 PE=4 SV=1 |
195| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL15_PSEPK | 59 | 50S ribosomal protein L15 OS=Pseudomonas putida (strain KT2440) GN=rplO PE=3 SV=1 |
196| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RECA_PSEPK | 59 | Protein RecA OS=Pseudomonas putida (strain KT2440) GN=recA PE=3 SV=1 |
197| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88KP1_PSEPK | 57 | Methyl-accepting chemotaxis transducer OS=Pseudomonas putida (strain KT2440) GN=PP_2249 PE=4 SV=1 | |
| 3 | Q88KI0_PSEPK | 42 | Methyl-accepting chemotaxis transducer OS=Pseudomonas putida (strain KT2440) GN=PP_2310 PE=4 SV=1 | |
| 2 | Q88R17_PSEPK | 44 | Methyl-accepting chemotaxis transducer OS=Pseudomonas putida (strain KT2440) GN=PP_0317 PE=4 SV=1 | |
198| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88GF9_PSEPK | 57 | Transcriptional regulator MvaT, P16 subunit, putative OS=Pseudomonas putida (strain KT2440) GN=PP_3765 PE=4 SV=1 |
199| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | PPK_PSEPK | 56 | Polyphosphate kinase OS=Pseudomonas putida (strain KT2440) GN=ppk PE=3 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 199.1 | PPK_PSEPK |
56 | 81966 | 8 (1) | 7 (1) | 0.03 | |
| Polyphosphate kinase OS=Pseudomonas putida (strain KT2440) GN=ppk PE=3 SV=1 | |||||||
8 peptide matches (7 non-duplicate, 1 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 212 |
487.3255 | 486.3182 | 486.3166 | 3.39 | 0 | 15 | 0.46 | 6Score > 24 indicates identity |
R.VAGLK.K | ||
| 491 |
354.6884 | 707.3622 | 707.3490 | 18.7 | 0 | 3 | 2.2 | 5Score > 22 indicates identity Score > 19 indicates homology |
U | E.TQFALE.G | |
| 914 |
1 |
450.7825 | 899.5504 | 899.5440 | 7.16 | 1 | 7 | 0.51 | 4Score > 17 indicates identity |
U | E.VTAVIELR.A |
| 1178 |
506.7883 | 1011.5620 | 1011.5713 | -9.12 | 0 | 1 | 3 | 3Score > 18 indicates identity |
U | R.LSNPVLNVR.- + Deamidated (NQ) | |
| 2208 |
752.9343 | 1503.8540 | 1503.8297 | 16.2 | 0 | 56 | 4.1e-006 | 1Score > 15 indicates identity |
U | K.LLANADNIFSVISK.Q | |
| 4015 |
871.1652 | 2610.4738 | 2610.4309 | 16.4 | 1 | 4 | 0.44 | 1Score > 13 indicates identity |
U | R.DEIAPIITPIGLDPTHPFPLLVNK.S + Deamidated (NQ) | |
| 4060 |
891.8101 | 2672.4085 | 2672.3738 | 13.0 | 1 | 4 | 1.1 | 1Score > 17 indicates identity |
U | R.LFSITGLDSHPELQYTPFTPAIPK.L + Deamidated (NQ) |
200| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | OTCC_PSEPK | 56 | Ornithine carbamoyltransferase, catabolic OS=Pseudomonas putida (strain KT2440) GN=arcB PE=3 SV=3 |
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