MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 12 Aug 2025 at 22:47:09 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 191–200 (out of 313)

Page: Previous 1 …15 16 17 18 19 20 21 22 23 24 25 … 32 Next 

-191

Accession Score Description
1 CLPX_PSEPK 60 ATP-dependent Clp protease ATP-binding subunit ClpX OS=Pseudomonas putida (strain KT2440) GN=clpX PE=3 SV=1
Score Mass Matches Sequences emPAI
191.1 CLPX_PSEPK 60 48952 8 (3) 7 (3) 0.15
ATP-dependent Clp protease ATP-binding subunit ClpX OS=Pseudomonas putida (strain KT2440) GN=clpX PE=3 SV=1

-8 peptide matches (8 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
585   380.2203 758.4260 758.4286 -3.39 1 5 5 +10Score > 24 indicates identity U E.KVIQNR.S + 2 Deamidated (NQ)
1122   494.2982 986.5818 986.5760 5.91 1 28 0.0048 +1Score > 17 indicates identity U K.TLLAETLAR.L
1231   519.2873 1036.5600 1036.5553 4.61 1 1 3.2 +2Score > 18 indicates identity U K.NALTKQYAK.L + Deamidated (NQ)
1440   567.8240 1133.6334 1133.6233 8.92 1 29 0.0036 +1Score > 17 indicates identity U K.FGLIPEFVGR.L
1655   410.9017 1229.6833 1229.6689 11.7 0 8 0.89 +2Score > 20 indicates identity U E.AALMQILTEPK.N + Oxidation (M)
1702   628.8685 1255.7224 1255.7136 7.05 0 38 0.00064 +1Score > 18 indicates identity U K.SNILLIGPTGSGK.T
2055   717.8641 1433.7136 1433.7402 -18.5 0 1 1 +1Score > 21 indicates identity
Score > 14 indicates homology		 U E.ISGILDQYVIGQE.R
2057   718.3665 1434.7184 1434.7242 -4.02 0 0 2.3 +4Score > 21 indicates identity
Score > 16 indicates homology		 U E.ISGILDQYVIGQE.R + Deamidated (NQ)

+192

Accession Score Description
1 Q88RK3_PSEPK 60 Cytochrome c4 OS=Pseudomonas putida (strain KT2440) GN=PP_0126 PE=4 SV=1

+193

Accession Score Description
1 Q88K29_PSEPK 59 Cold shock protein CspA OS=Pseudomonas putida (strain KT2440) GN=cspA-2 PE=4 SV=1

+194

Accession Score Description
1 Q88Q14_PSEPK 59 Peptidyl-prolyl cis-trans isomerase OS=Pseudomonas putida (strain KT2440) GN=fklB-1 PE=4 SV=1

+195

Accession Score Description
1 RL15_PSEPK 59 50S ribosomal protein L15 OS=Pseudomonas putida (strain KT2440) GN=rplO PE=3 SV=1

+196

Accession Score Description
1 RECA_PSEPK 59 Protein RecA OS=Pseudomonas putida (strain KT2440) GN=recA PE=3 SV=1

+197

Accession Score Description
Family member distances as a dendrogram 1 Q88KP1_PSEPK 57 Methyl-accepting chemotaxis transducer OS=Pseudomonas putida (strain KT2440) GN=PP_2249 PE=4 SV=1
3 Q88KI0_PSEPK 42 Methyl-accepting chemotaxis transducer OS=Pseudomonas putida (strain KT2440) GN=PP_2310 PE=4 SV=1
2 Q88R17_PSEPK 44 Methyl-accepting chemotaxis transducer OS=Pseudomonas putida (strain KT2440) GN=PP_0317 PE=4 SV=1

+198

Accession Score Description
1 Q88GF9_PSEPK 57 Transcriptional regulator MvaT, P16 subunit, putative OS=Pseudomonas putida (strain KT2440) GN=PP_3765 PE=4 SV=1

+199

Accession Score Description
1 PPK_PSEPK 56 Polyphosphate kinase OS=Pseudomonas putida (strain KT2440) GN=ppk PE=3 SV=1

+200

Accession Score Description
1 OTCC_PSEPK 56 Ornithine carbamoyltransferase, catabolic OS=Pseudomonas putida (strain KT2440) GN=arcB PE=3 SV=3
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