MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 12 Aug 2025 at 22:47:09 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 11–20 (out of 313)

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+11

Accession Score Description
Family member distances as a dendrogram 1 Q88NK1_PSEPK 752 Porin D OS=Pseudomonas putida (strain KT2440) GN=oprD PE=4 SV=1
3 Q88MB8_PSEPK 45 GTP pyrophosphokinase OS=Pseudomonas putida (strain KT2440) GN=relA PE=3 SV=1
2 Q88R66_PSEPK 273 Outer membrane protein OprE3 OS=Pseudomonas putida (strain KT2440) GN=oprQ PE=4 SV=1

+12

Accession Score Description
1 Q88FA9_PSEPK 748 2-oxoglutarate dehydrogenase, E1 component OS=Pseudomonas putida (strain KT2440) GN=kgdA PE=4 SV=1

+13

Accession Score Description
1 RPOC_PSEPK 704 DNA-directed RNA polymerase subunit beta' OS=Pseudomonas putida (strain KT2440) GN=rpoC PE=3 SV=1

+14

Accession Score Description
1 RL3_PSEPK 676 50S ribosomal protein L3 OS=Pseudomonas putida (strain KT2440) GN=rplC PE=3 SV=2

+15

Accession Score Description
1 Q88FB1_PSEPK 674 Dihydrolipoyl dehydrogenase OS=Pseudomonas putida (strain KT2440) GN=lpdG PE=4 SV=1

+16

Accession Score Description
1 RS2_PSEPK 613 30S ribosomal protein S2 OS=Pseudomonas putida (strain KT2440) GN=rpsB PE=3 SV=1

+17

Accession Score Description
1 RS5_PSEPK 611 30S ribosomal protein S5 OS=Pseudomonas putida (strain KT2440) GN=rpsE PE=3 SV=1

+18

Accession Score Description
1 Q88R99_PSEPK 608 Porin E OS=Pseudomonas putida (strain KT2440) GN=oprE PE=4 SV=1

-19

Accession Score Description
1 CH60_PSEPK 589 60 kDa chaperonin OS=Pseudomonas putida (strain KT2440) GN=groL PE=3 SV=1
Score Mass Matches Sequences emPAI
19.1 CH60_PSEPK 589 56765 34 (23) 22 (16) 1.29
60 kDa chaperonin OS=Pseudomonas putida (strain KT2440) GN=groL PE=3 SV=1

-34 peptide matches (30 non-duplicate, 4 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
212   487.3255 486.3182 486.3166 3.39 0 18 0.22 +5Score > 24 indicates identity U K.VAAVK.A
277   586.3574 585.3501 585.3486 2.60 0 36 0.0016 +1Score > 20 indicates identity U K.ATLGPK.G
301 +1 301.6854 601.3562 601.3547 2.51 0 22 0.081 +2Score > 23 indicates identity U K.ISNIR.E
302   602.3646 601.3573 601.3547 4.30 0 22 0.076 +1Score > 23 indicates identity U K.ISNIR.E
360   322.2114 642.4082 642.4177 -14.7 1 1 4.7 +7Score > 21 indicates identity
Score > 20 indicates homology		 R.VKQIR.A
361   643.4159 642.4086 642.4064 3.39 0 40 0.00079 +1Score > 21 indicates identity U R.NVVLAK.S
362   322.2117 642.4088 642.4064 3.74 0 10 0.78 +4Score > 21 indicates identity U R.NVVLAK.S
365   322.7088 643.4030 643.4017 2.11 1 9 0.65 +1Score > 23 indicates identity
Score > 20 indicates homology		 R.VKQIR.A + Deamidated (NQ)
377   326.6575 651.3004 651.2976 4.31 0 16 0.12 +1Score > 20 indicates identity U K.FGDSAR.K
421   338.1859 674.3572 674.3599 -3.95 0 28 0.015 +1Score > 22 indicates identity U K.DGVSVAK.E
422   675.3698 674.3625 674.3599 3.88 0 44 0.00035 +1Score > 22 indicates identity U K.DGVSVAK.E
508   360.1780 718.3414 718.3398 2.23 0 29 0.0098 +1Score > 21 indicates identity U K.APGFGDR.R
534   365.7331 729.4516 729.4497 2.68 1 15 0.15 +1Score > 19 indicates identity U K.KISNIR.E
609   386.2227 770.4308 770.4286 2.87 1 26 0.0097 +1Score > 19 indicates identity U R.AVESPLR.Q
728   827.5371 826.5298 826.5276 2.65 0 42 0.00028 +1Score > 19 indicates identity U K.LAGGVAVIK.V
730   414.2726 826.5306 826.5276 3.65 0 19 0.031 +1Score > 16 indicates identity U K.LAGGVAVIK.V
958   921.5068 920.4995 920.4967 3.03 0 52 4.1e-005 +1Score > 21 indicates identity U K.SFGAPTITK.D
959   461.2576 920.5006 920.4967 4.25 0 33 0.0012 +1Score > 21 indicates identity
Score > 16 indicates homology		 U K.SFGAPTITK.D
975 +1 464.3014 926.5882 926.5800 8.87 0 54 3.7e-006 +1Score > 13 indicates identity U R.ALAAIIDLK.G
1096 +1 486.7919 971.5692 971.5651 4.24 1 55 1.4e-005 +1Score > 19 indicates identity U K.ATAAVVAELK.N
1173   337.8465 1010.5177 1010.5145 3.14 1 43 0.00025 +1Score > 19 indicates identity U R.VEDALHATR.A
1174   506.2665 1010.5184 1010.5145 3.91 1 63 2e-006 +1Score > 19 indicates identity U R.VEDALHATR.A
1388   560.2787 1118.5428 1118.5390 3.45 0 1 5.5 +8Score > 21 indicates identity U K.NLSKPCADSK.A
1827   661.8278 1321.6410 1321.6336 5.62 1 35 0.00069 +1Score > 21 indicates identity
Score > 16 indicates homology		 U K.DAFENMGAQLVK.E
1836   664.8887 1327.7628 1327.7534 7.15 0 57 4.7e-006 +1Score > 16 indicates identity U K.MLVGVNVLADAVK.A
2223   757.3872 1512.7598 1512.7532 4.38 1 76 1.8e-007 +1Score > 21 indicates identity U K.GDNEDQNVGIALLR.R
2224   505.2607 1512.7603 1512.7532 4.66 1 52 4.6e-005 +1Score > 21 indicates identity U K.GDNEDQNVGIALLR.R
2433   807.9001 1613.7856 1613.7897 -2.49 0 67 1.5e-006 +1Score > 21 indicates identity U R.QITANAGDEPSVVADK.V
2919   614.6603 1840.9591 1840.9530 3.27 1 39 0.00065 +1Score > 19 indicates identity U R.QITANAGDEPSVVADKVK.Q
3832 +1 801.4170 2401.2292 2401.1973 13.3 1 20 0.054 +1Score > 19 indicates identity U K.ANDAAGDGTTTATVLAQAIVNEGLK.A + Deamidated (NQ)

+20

Accession Score Description
1 Q88PD7_PSEPK 581 Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_0913 PE=4 SV=1
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