MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita1 |
| MS data file | : | PRT1270_T-BRSC_1_20250714114344.mgf |
| Database | : | P-putida 20180924 (5,556 sequences; 1,892,173 residues) |
| Timestamp | : | 12 Aug 2025 at 22:47:09 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,938 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
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181| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88P99_PSEPK | 68 | ABC transporter, ATP-binding protein, putative OS=Pseudomonas putida (strain KT2440) GN=PP_0953 PE=3 SV=1 |
182| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88NY5_PSEPK | 67 | Amino acid ABC transporter, ATP-binding protein OS=Pseudomonas putida (strain KT2440) GN=PP_1068 PE=3 SV=1 |
183| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88RL8_PSEPK | 66 | Sco1/SenC family protein OS=Pseudomonas putida (strain KT2440) GN=PP_0111 PE=4 SV=1 |
184| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88EI3_PSEPK | 65 | Arginine N-succinyltransferase OS=Pseudomonas putida (strain KT2440) GN=aruG PE=4 SV=1 |
185| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88NI8_PSEPK | 65 | Biopolymer transport protein TolQ OS=Pseudomonas putida (strain KT2440) GN=tolQ PE=3 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 185.1 | Q88NI8_PSEPK |
65 | 25294 | 3 (2) | 3 (2) | 0.19 | |
| Biopolymer transport protein TolQ OS=Pseudomonas putida (strain KT2440) GN=tolQ PE=3 SV=1 | |||||||
3 peptide matches (3 non-duplicate, 0 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 954 |
919.5299 | 918.5226 | 918.5069 | 17.1 | 1 | 3 | 1.7 | 9Score > 18 indicates identity |
U | R.AMRVAISR.E + Oxidation (M) | |
| 1251 |
526.7775 | 1051.5404 | 1051.5338 | 6.28 | 0 | 30 | 0.0027 | 1Score > 20 indicates identity Score > 17 indicates homology |
U | R.FWSGIDLSK.L | |
| 2798 |
592.2960 | 1773.8662 | 1773.8362 | 16.9 | 1 | 54 | 2.4e-005 | 1Score > 21 indicates identity |
U | R.YYTFADEFQAILHR.K + Deamidated (NQ) |
186| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ATP6_PSEPK | 63 | ATP synthase subunit a OS=Pseudomonas putida (strain KT2440) GN=atpB PE=3 SV=1 |
187| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88FQ0_PSEPK | 63 | Dihydroorotate dehydrogenase family protein OS=Pseudomonas putida (strain KT2440) GN=PP_4038 PE=4 SV=1 |
188| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88G86_PSEPK | 62 | Alcohol dehydrogenase, zinc-containing OS=Pseudomonas putida (strain KT2440) GN=PP_3839 PE=3 SV=1 |
189| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | DAVD_PSEPK | 60 | Glutarate-semialdehyde dehydrogenase DavD OS=Pseudomonas putida (strain KT2440) GN=davD PE=3 SV=1 |
190| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | HTPX_PSEPK | 60 | Protease HtpX OS=Pseudomonas putida (strain KT2440) GN=htpX PE=3 SV=1 |
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