MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 12 Aug 2025 at 22:47:09 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 181–190 (out of 313)


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-181

Accession Score Description
1 Q88P99_PSEPK 68 ABC transporter, ATP-binding protein, putative OS=Pseudomonas putida (strain KT2440) GN=PP_0953 PE=3 SV=1
Score Mass Matches Sequences emPAI
181.1 Q88P99_PSEPK 68 26677 2 (1) 2 (1) 0.09
ABC transporter, ATP-binding protein, putative OS=Pseudomonas putida (strain KT2440) GN=PP_0953 PE=3 SV=1

-2 peptide matches (2 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
511   361.2166 720.4186 720.4282 -13.3 1 6 0.61 +2Score > 17 indicates identity U E.ASIFRK.L
2242   761.4117 1520.8088 1520.7987 6.67 1 68 7.1e-007 +1Score > 19 indicates identity U R.AGIGYLPQEASIFR.K

+182

Accession Score Description
1 Q88NY5_PSEPK 67 Amino acid ABC transporter, ATP-binding protein OS=Pseudomonas putida (strain KT2440) GN=PP_1068 PE=3 SV=1

+183

Accession Score Description
1 Q88RL8_PSEPK 66 Sco1/SenC family protein OS=Pseudomonas putida (strain KT2440) GN=PP_0111 PE=4 SV=1

+184

Accession Score Description
1 Q88EI3_PSEPK 65 Arginine N-succinyltransferase OS=Pseudomonas putida (strain KT2440) GN=aruG PE=4 SV=1

+185

Accession Score Description
1 Q88NI8_PSEPK 65 Biopolymer transport protein TolQ OS=Pseudomonas putida (strain KT2440) GN=tolQ PE=3 SV=1

+186

Accession Score Description
1 ATP6_PSEPK 63 ATP synthase subunit a OS=Pseudomonas putida (strain KT2440) GN=atpB PE=3 SV=1

+187

Accession Score Description
1 Q88FQ0_PSEPK 63 Dihydroorotate dehydrogenase family protein OS=Pseudomonas putida (strain KT2440) GN=PP_4038 PE=4 SV=1

+188

Accession Score Description
1 Q88G86_PSEPK 62 Alcohol dehydrogenase, zinc-containing OS=Pseudomonas putida (strain KT2440) GN=PP_3839 PE=3 SV=1

+189

Accession Score Description
1 DAVD_PSEPK 60 Glutarate-semialdehyde dehydrogenase DavD OS=Pseudomonas putida (strain KT2440) GN=davD PE=3 SV=1

+190

Accession Score Description
1 HTPX_PSEPK 60 Protease HtpX OS=Pseudomonas putida (strain KT2440) GN=htpX PE=3 SV=1
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