MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 12 Aug 2025 at 22:47:09 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 11–20 (out of 313)

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+11

Accession Score Description
Family member distances as a dendrogram 1 Q88NK1_PSEPK 752 Porin D OS=Pseudomonas putida (strain KT2440) GN=oprD PE=4 SV=1
3 Q88MB8_PSEPK 45 GTP pyrophosphokinase OS=Pseudomonas putida (strain KT2440) GN=relA PE=3 SV=1
2 Q88R66_PSEPK 273 Outer membrane protein OprE3 OS=Pseudomonas putida (strain KT2440) GN=oprQ PE=4 SV=1

+12

Accession Score Description
1 Q88FA9_PSEPK 748 2-oxoglutarate dehydrogenase, E1 component OS=Pseudomonas putida (strain KT2440) GN=kgdA PE=4 SV=1

+13

Accession Score Description
1 RPOC_PSEPK 704 DNA-directed RNA polymerase subunit beta' OS=Pseudomonas putida (strain KT2440) GN=rpoC PE=3 SV=1

+14

Accession Score Description
1 RL3_PSEPK 676 50S ribosomal protein L3 OS=Pseudomonas putida (strain KT2440) GN=rplC PE=3 SV=2

+15

Accession Score Description
1 Q88FB1_PSEPK 674 Dihydrolipoyl dehydrogenase OS=Pseudomonas putida (strain KT2440) GN=lpdG PE=4 SV=1

+16

Accession Score Description
1 RS2_PSEPK 613 30S ribosomal protein S2 OS=Pseudomonas putida (strain KT2440) GN=rpsB PE=3 SV=1

+17

Accession Score Description
1 RS5_PSEPK 611 30S ribosomal protein S5 OS=Pseudomonas putida (strain KT2440) GN=rpsE PE=3 SV=1

-18

Accession Score Description
1 Q88R99_PSEPK 608 Porin E OS=Pseudomonas putida (strain KT2440) GN=oprE PE=4 SV=1
Score Mass Matches Sequences emPAI
18.1 Q88R99_PSEPK 608 48287 30 (22) 20 (17) 1.53
Porin E OS=Pseudomonas putida (strain KT2440) GN=oprE PE=4 SV=1

-30 peptide matches (27 non-duplicate, 3 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
329   311.6704 621.3262 621.3235 4.49 0 31 0.0036 +1Score > 19 indicates identity U R.QGVYR.G
389   331.6962 661.3778 661.3759 2.99 0 23 0.013 +1Score > 17 indicates identity U K.LSLSSR.T
569   749.4335 748.4262 748.4232 4.08 0 11 0.38 +2Score > 19 indicates identity U K.GLGFSLR.Q
570   375.2206 748.4266 748.4232 4.64 0 21 0.034 +1Score > 19 indicates identity U K.GLGFSLR.Q
646   786.4484 785.4411 785.4508 -12.3 1 19 0.13 +9Score > 23 indicates identity U R.IGGNAKAR.F
647 +1 393.7281 785.4416 785.4508 -11.6 1 12 0.59 +4Score > 23 indicates identity U R.IGGNAKAR.F
669   398.2228 794.4310 794.4286 3.04 0 47 9.1e-005 +1Score > 19 indicates identity U K.ASVSYLR.G
670   795.4384 794.4311 794.4286 3.13 0 14 0.18 +1Score > 19 indicates identity U K.ASVSYLR.G
960   461.7365 921.4584 921.4556 3.11 0 37 0.0014 +1Score > 21 indicates identity U K.IADNQSFK.T
1131   497.7708 993.5270 993.5244 2.71 0 64 1.5e-006 +1Score > 18 indicates identity U R.TGLSVSGAFR.D
1162   503.2131 1004.4116 1004.4087 2.93 0 47 4.5e-005 +1Score > 16 indicates identity U R.YFDSSSDGK.N
1211   516.7448 1031.4750 1031.4713 3.68 0 37 0.00051 +1Score > 20 indicates identity
Score > 16 indicates homology		 U K.FQFAGGDYK.L
1716   633.8346 1265.6546 1265.6503 3.41 1 55 1.7e-005 +1Score > 20 indicates identity U R.VDYTLQEGTLK.G
1757   321.9276 1283.6813 1283.6775 2.95 0 11 0.38 +1Score > 19 indicates identity U K.QHFLGATHVFK.I
1758   428.9015 1283.6827 1283.6775 4.03 0 30 0.0045 +1Score > 19 indicates identity U K.QHFLGATHVFK.I
1773   647.3336 1292.6526 1292.6441 6.60 0 14 0.27 +1Score > 21 indicates identity U R.FIVNYTYNFL.-
2130   492.8982 1475.6728 1475.6681 3.16 1 36 0.0013 +1Score > 19 indicates identity U R.DSNKFQFAGGDYK.L
2234 +2 759.9152 1517.8158 1517.8090 4.54 1 47 8.7e-005 +1Score > 19 indicates identity U R.LLPQTFEGGTLQSK.E
2235   506.9471 1517.8195 1517.8090 6.93 1 29 0.0062 +1Score > 19 indicates identity U R.LLPQTFEGGTLQSK.E
3763   781.0790 2340.2152 2340.1784 15.7 1 46 8.8e-005 +1Score > 18 indicates identity U K.TEVHYGSALAPMLPILVSNDGR.L + Deamidated (NQ)
3814   1194.0863 2386.1580 2386.1256 13.6 1 110 4.7e-011 +1Score > 20 indicates identity U K.LTPDLTLQYYYSNLEDFYK.Q + Deamidated (NQ)
3815   796.3943 2386.1611 2386.1256 14.9 1 27 0.011 +1Score > 20 indicates identity U K.LTPDLTLQYYYSNLEDFYK.Q + Deamidated (NQ)
3837   803.0618 2406.1636 2406.1380 10.6 1 17 0.086 +1Score > 19 indicates identity U R.AGENTTFGQYSYDFAALGVPGLK.A + Deamidated (NQ)
4056   889.0435 2664.1087 2664.0888 7.45 1 57 1.8e-006 +1Score > 13 indicates identity U R.YFDSSSDGKNGQTGYAFDNNGGYAK.N + 2 Deamidated (NQ)
4097   903.0733 2706.1981 2706.1695 10.6 0 68 3e-007 +1Score > 15 indicates identity U R.GSRPDANNTFFPSDSDGSAVHDWAR.I + Deamidated (NQ)
4098   677.5579 2706.2025 2706.1695 12.2 0 61 1.7e-006 +1Score > 16 indicates identity U R.GSRPDANNTFFPSDSDGSAVHDWAR.I + Deamidated (NQ)
4458   1060.5470 3178.6192 3178.5724 14.7 0 23 0.018 +1Score > 18 indicates identity U K.LDYLSGFTQGTVGFGVDVQALWGIHLDGGR.G + Deamidated (NQ)

+19

Accession Score Description
1 CH60_PSEPK 589 60 kDa chaperonin OS=Pseudomonas putida (strain KT2440) GN=groL PE=3 SV=1

+20

Accession Score Description
1 Q88PD7_PSEPK 581 Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_0913 PE=4 SV=1
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