MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita1 |
| MS data file | : | PRT1270_T-BRSC_1_20250714114344.mgf |
| Database | : | P-putida 20180924 (5,556 sequences; 1,892,173 residues) |
| Timestamp | : | 12 Aug 2025 at 22:47:09 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,938 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
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11| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88NK1_PSEPK | 752 | Porin D OS=Pseudomonas putida (strain KT2440) GN=oprD PE=4 SV=1 | |
| 3 | Q88MB8_PSEPK | 45 | GTP pyrophosphokinase OS=Pseudomonas putida (strain KT2440) GN=relA PE=3 SV=1 | |
| 2 | Q88R66_PSEPK | 273 | Outer membrane protein OprE3 OS=Pseudomonas putida (strain KT2440) GN=oprQ PE=4 SV=1 | |
12| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88FA9_PSEPK | 748 | 2-oxoglutarate dehydrogenase, E1 component OS=Pseudomonas putida (strain KT2440) GN=kgdA PE=4 SV=1 |
13| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RPOC_PSEPK | 704 | DNA-directed RNA polymerase subunit beta' OS=Pseudomonas putida (strain KT2440) GN=rpoC PE=3 SV=1 |
14| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL3_PSEPK | 676 | 50S ribosomal protein L3 OS=Pseudomonas putida (strain KT2440) GN=rplC PE=3 SV=2 |
15| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88FB1_PSEPK | 674 | Dihydrolipoyl dehydrogenase OS=Pseudomonas putida (strain KT2440) GN=lpdG PE=4 SV=1 |
16| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RS2_PSEPK | 613 | 30S ribosomal protein S2 OS=Pseudomonas putida (strain KT2440) GN=rpsB PE=3 SV=1 |
17| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RS5_PSEPK | 611 | 30S ribosomal protein S5 OS=Pseudomonas putida (strain KT2440) GN=rpsE PE=3 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 17.1 | RS5_PSEPK |
611 | 17712 | 17 (14) | 7 (5) | 1.71 | |
| 30S ribosomal protein S5 OS=Pseudomonas putida (strain KT2440) GN=rpsE PE=3 SV=1 | |||||||
17 peptide matches (11 non-duplicate, 6 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
| 246 |
535.3007 | 534.2934 | 534.2914 | 3.71 | 0 | 12 | 0.24 | 1Score > 18 indicates identity |
U | R.VGFGR.G | |
| 526 |
1 |
364.7251 | 727.4356 | 727.4341 | 2.17 | 0 | 37 | 0.00086 | 1Score > 19 indicates identity |
U | K.LVQVNR.V |
| 527 |
728.4432 | 727.4359 | 727.4341 | 2.55 | 0 | 30 | 0.0045 | 1Score > 19 indicates identity |
U | K.LVQVNR.V | |
| 811 |
855.4952 | 854.4879 | 854.4861 | 2.08 | 1 | 57 | 7.4e-006 | 1Score > 18 indicates identity |
U | R.EVPAAIQK.A | |
| 812 |
428.2514 | 854.4882 | 854.4861 | 2.46 | 1 | 20 | 0.039 | 1Score > 18 indicates identity |
U | R.EVPAAIQK.A | |
| 1107 |
488.7600 | 975.5054 | 975.5059 | -0.47 | 0 | 4 | 3.5 | 6Score > 22 indicates identity |
U | R.NMIQVDLK.G + Oxidation (M) | |
| 1117 |
982.5229 | 981.5156 | 981.5131 | 2.56 | 0 | 75 | 9.2e-008 | 1Score > 17 indicates identity |
U | K.GTTLQYATK.A | |
| 1118 |
491.7656 | 981.5166 | 981.5131 | 3.61 | 0 | 38 | 0.00053 | 1Score > 18 indicates identity |
U | K.GTTLQYATK.A | |
| 2002 |
470.9525 | 1409.8357 | 1409.8242 | 8.14 | 1 | 3 | 0.69 | 1Score > 14 indicates identity |
U | R.AVLEVAGVQNVLAK.C | |
| 2004 |
2 |
705.9265 | 1409.8384 | 1409.8242 | 10.1 | 1 | 75 | 4.9e-008 | 1Score > 14 indicates identity |
U | R.AVLEVAGVQNVLAK.C |
| 2104 |
3 |
734.9117 | 1467.8088 | 1467.7974 | 7.82 | 0 | 104 | 1.3e-010 | 1Score > 18 indicates identity |
U | R.IFTFTALTVVGDGK.G |
18| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88R99_PSEPK | 608 | Porin E OS=Pseudomonas putida (strain KT2440) GN=oprE PE=4 SV=1 |
19| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | CH60_PSEPK | 589 | 60 kDa chaperonin OS=Pseudomonas putida (strain KT2440) GN=groL PE=3 SV=1 |
20| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88PD7_PSEPK | 581 | Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_0913 PE=4 SV=1 |
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