MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita1 |
| MS data file | : | PRT1270_T-BRSC_1_20250714114344.mgf |
| Database | : | P-putida 20180924 (5,556 sequences; 1,892,173 residues) |
| Timestamp | : | 12 Aug 2025 at 22:47:09 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,938 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
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161| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RS6_PSEPK | 81 | 30S ribosomal protein S6 OS=Pseudomonas putida (strain KT2440) GN=rpsF PE=3 SV=1 |
162| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | PURA_PSEPK | 81 | Adenylosuccinate synthetase OS=Pseudomonas putida (strain KT2440) GN=purA PE=3 SV=1 |
163| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88FA0_PSEPK | 80 | OmpA family protein OS=Pseudomonas putida (strain KT2440) GN=PP_4198 PE=3 SV=1 |
164| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88N50_PSEPK | 80 | Transcriptional regulator MvaT, P16 subunit OS=Pseudomonas putida (strain KT2440) GN=PP_1366 PE=4 SV=1 |
165| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88RW6_PSEPK | 80 | DNA gyrase subunit B OS=Pseudomonas putida (strain KT2440) GN=gyrB PE=3 SV=1 |
166| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ACP_PSEPK | 79 | Acyl carrier protein OS=Pseudomonas putida (strain KT2440) GN=acpP PE=3 SV=1 |
167| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | HSLU_PSEPK | 79 | ATP-dependent protease ATPase subunit HslU OS=Pseudomonas putida (strain KT2440) GN=hslU PE=3 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 167.1 | HSLU_PSEPK |
79 | 50008 | 5 (2) | 5 (2) | 0.09 | |
| ATP-dependent protease ATPase subunit HslU OS=Pseudomonas putida (strain KT2440) GN=hslU PE=3 SV=1 | |||||||
5 peptide matches (5 non-duplicate, 0 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 420 |
673.4290 | 672.4217 | 672.4170 | 7.01 | 1 | 7 | 0.54 | 8Score > 17 indicates identity |
U | E.IDKVAK.R | |
| 598 |
382.2271 | 762.4396 | 762.4276 | 15.8 | 0 | 10 | 0.41 | 6Score > 18 indicates identity |
U | E.FLADGIK.R | |
| 852 |
437.7545 | 873.4944 | 873.4960 | -1.74 | 0 | 4 | 2.4 | 6Score > 20 indicates identity |
U | K.LANAPFLK.V + Deamidated (NQ) | |
| 1374 |
555.3433 | 1108.6720 | 1108.6604 | 10.5 | 0 | 47 | 2e-005 | 1Score > 13 indicates identity |
U | R.ILDALLPQAR.V | |
| 3306 |
695.0541 | 2082.1405 | 2082.0997 | 19.6 | 1 | 46 | 5e-005 | 1Score > 15 indicates identity |
U | R.ILQEPHASLTEQYQALLK.T + Deamidated (NQ) |
168| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88N93_PSEPK | 78 | Ubiquinol--cytochrome c reductase, cytochrome c1 OS=Pseudomonas putida (strain KT2440) GN=petC PE=4 SV=1 |
169| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88G04_PSEPK | 78 | SohB protein, peptidase U7 family OS=Pseudomonas putida (strain KT2440) GN=PP_3922 PE=4 SV=1 |
170| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88C80_PSEPK | 76 | Endoribonuclease OS=Pseudomonas putida (strain KT2440) GN=PP_5303 PE=4 SV=1 |
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