MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 12 Aug 2025 at 22:47:09 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 161–170 (out of 313)


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-161

Accession Score Description
1 RS6_PSEPK 81 30S ribosomal protein S6 OS=Pseudomonas putida (strain KT2440) GN=rpsF PE=3 SV=1
Score Mass Matches Sequences emPAI
161.1 RS6_PSEPK 81 16420 5 (4) 4 (3) 0.71
30S ribosomal protein S6 OS=Pseudomonas putida (strain KT2440) GN=rpsF PE=3 SV=1

-5 peptide matches (5 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
315   307.7038 613.3930 613.3911 3.13 0 23 0.036 +1Score > 21 indicates identity U R.NLVIR.R
615   388.1916 774.3686 774.3660 3.36 1 20 0.063 +1Score > 21 indicates identity
Score > 20 indicates homology
U R.LEDWGR.R
789   425.7253 849.4360 849.4344 1.88 0 35 0.0028 +1Score > 22 indicates identity U R.YNDAVIR.N
1725   635.8506 1269.6866 1269.6829 2.92 0 59 3.9e-006 +1Score > 21 indicates identity
Score > 18 indicates homology
U R.QLAYAINNVHK.A
1726   424.2365 1269.6877 1269.6829 3.73 0 26 0.021 +1Score > 21 indicates identity U R.QLAYAINNVHK.A

1 subset or intersection (1 subset protein in total)

Score Mass Subset of
Q88DW4_PSEPK 23 0 161.1
description

+162

Accession Score Description
1 PURA_PSEPK 81 Adenylosuccinate synthetase OS=Pseudomonas putida (strain KT2440) GN=purA PE=3 SV=1

+163

Accession Score Description
1 Q88FA0_PSEPK 80 OmpA family protein OS=Pseudomonas putida (strain KT2440) GN=PP_4198 PE=3 SV=1

+164

Accession Score Description
1 Q88N50_PSEPK 80 Transcriptional regulator MvaT, P16 subunit OS=Pseudomonas putida (strain KT2440) GN=PP_1366 PE=4 SV=1

+165

Accession Score Description
1 Q88RW6_PSEPK 80 DNA gyrase subunit B OS=Pseudomonas putida (strain KT2440) GN=gyrB PE=3 SV=1

+166

Accession Score Description
1 ACP_PSEPK 79 Acyl carrier protein OS=Pseudomonas putida (strain KT2440) GN=acpP PE=3 SV=1

+167

Accession Score Description
1 HSLU_PSEPK 79 ATP-dependent protease ATPase subunit HslU OS=Pseudomonas putida (strain KT2440) GN=hslU PE=3 SV=1

+168

Accession Score Description
1 Q88N93_PSEPK 78 Ubiquinol--cytochrome c reductase, cytochrome c1 OS=Pseudomonas putida (strain KT2440) GN=petC PE=4 SV=1

+169

Accession Score Description
1 Q88G04_PSEPK 78 SohB protein, peptidase U7 family OS=Pseudomonas putida (strain KT2440) GN=PP_3922 PE=4 SV=1

+170

Accession Score Description
1 Q88C80_PSEPK 76 Endoribonuclease OS=Pseudomonas putida (strain KT2440) GN=PP_5303 PE=4 SV=1
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