MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 12 Aug 2025 at 22:47:09 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 11–20 (out of 313)

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+11

Accession Score Description
Family member distances as a dendrogram 1 Q88NK1_PSEPK 752 Porin D OS=Pseudomonas putida (strain KT2440) GN=oprD PE=4 SV=1
3 Q88MB8_PSEPK 45 GTP pyrophosphokinase OS=Pseudomonas putida (strain KT2440) GN=relA PE=3 SV=1
2 Q88R66_PSEPK 273 Outer membrane protein OprE3 OS=Pseudomonas putida (strain KT2440) GN=oprQ PE=4 SV=1

+12

Accession Score Description
1 Q88FA9_PSEPK 748 2-oxoglutarate dehydrogenase, E1 component OS=Pseudomonas putida (strain KT2440) GN=kgdA PE=4 SV=1

+13

Accession Score Description
1 RPOC_PSEPK 704 DNA-directed RNA polymerase subunit beta' OS=Pseudomonas putida (strain KT2440) GN=rpoC PE=3 SV=1

+14

Accession Score Description
1 RL3_PSEPK 676 50S ribosomal protein L3 OS=Pseudomonas putida (strain KT2440) GN=rplC PE=3 SV=2

+15

Accession Score Description
1 Q88FB1_PSEPK 674 Dihydrolipoyl dehydrogenase OS=Pseudomonas putida (strain KT2440) GN=lpdG PE=4 SV=1

-16

Accession Score Description
1 RS2_PSEPK 613 30S ribosomal protein S2 OS=Pseudomonas putida (strain KT2440) GN=rpsB PE=3 SV=1
Score Mass Matches Sequences emPAI
16.1 RS2_PSEPK 613 27063 32 (25) 12 (10) 3.04
30S ribosomal protein S2 OS=Pseudomonas putida (strain KT2440) GN=rpsB PE=3 SV=1

-32 peptide matches (24 non-duplicate, 8 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
232   518.2979 517.2906 517.2972 -12.8 1 1 1.7 +4Score > 24 indicates identity
Score > 15 indicates homology		 U K.RSAGK.I
487   354.1801 706.3456 706.3438 2.56 0 15 0.073 +1Score > 20 indicates identity
Score > 16 indicates homology		 U R.YWNPK.M
488   707.3535 706.3462 706.3438 3.37 0 28 0.0078 +1Score > 19 indicates identity U R.YWNPK.M
520   363.7015 725.3884 725.3860 3.31 0 14 0.062 +1Score > 15 indicates identity U K.YIFGAR.N
521   726.3965 725.3892 725.3860 4.38 0 21 0.012 +1Score > 15 indicates identity U K.YIFGAR.N
542   367.6849 733.3552 733.3541 1.57 0 11 0.58 +1Score > 21 indicates identity U M.SQVNMR.D
628 +3 389.2484 776.4822 776.4796 3.37 0 43 4.9e-005 +1Score > 13 indicates identity U K.ILFVGTK.R
630   777.4896 776.4823 776.4796 3.47 0 53 4.8e-006 +1Score > 13 indicates identity U K.ILFVGTK.R
815 +1 429.2466 856.4786 856.4766 2.35 1 41 0.00062 +1Score > 21 indicates identity U K.IVAEQAAR.C
816   857.4860 856.4787 856.4766 2.43 1 64 3.2e-006 +1Score > 21 indicates identity U K.IVAEQAAR.C
821   430.2484 858.4822 858.4810 1.40 1 42 0.0005 +1Score > 22 indicates identity U R.IAITEANK.L
822   859.4901 858.4828 858.4810 2.07 1 59 1.2e-005 +1Score > 22 indicates identity U R.IAITEANK.L
1086 +2 322.5318 964.5736 964.5705 3.14 1 27 0.0028 +1Score > 14 indicates identity U K.IHIVNLEK.T
1087   483.2946 964.5746 964.5705 4.26 1 18 0.024 +1Score > 14 indicates identity U K.IHIVNLEK.T
1372   370.5255 1108.5547 1108.5526 1.83 0 38 0.0012 +1Score > 21 indicates identity U K.AGVHFGHQTR.Y
1373   555.2849 1108.5552 1108.5526 2.35 0 18 0.12 +1Score > 21 indicates identity U K.AGVHFGHQTR.Y
1556   591.8077 1181.6008 1181.5903 8.93 0 31 0.0013 +1Score > 20 indicates identity
Score > 15 indicates homology		 U R.WLGGMLTNYK.T
2491   547.2792 1638.8158 1638.8076 5.00 1 82 3.6e-008 +1Score > 20 indicates identity
Score > 20 indicates homology		 U K.TLPMFNDALSFVER.L
2492 +1 820.4160 1638.8174 1638.8076 6.03 1 62 3.8e-006 +1Score > 20 indicates identity U K.TLPMFNDALSFVER.L
2495   820.9069 1639.7992 1639.7916 4.68 1 17 0.13 +1Score > 21 indicates identity U K.TLPMFNDALSFVER.L + Deamidated (NQ)
2532   828.4158 1654.8170 1654.8025 8.80 1 25 0.022 +1Score > 21 indicates identity U K.TLPMFNDALSFVER.L + Oxidation (M)
4400 +1 1032.8754 3095.6044 3095.5663 12.3 1 68 4.1e-007 +1Score > 16 indicates identity U K.LGIPVIGVVDTNSSPEGVDYIIPGNDDAIR.A + Deamidated (NQ)
4401   774.9089 3095.6065 3095.5663 13.0 1 37 0.00049 +1Score > 16 indicates identity U K.LGIPVIGVVDTNSSPEGVDYIIPGNDDAIR.A + Deamidated (NQ)
4402   1032.8773 3095.6101 3095.5663 14.1 1 44 8e-005 +1Score > 16 indicates identity U K.LGIPVIGVVDTNSSPEGVDYIIPGNDDAIR.A + Deamidated (NQ)

+17

Accession Score Description
1 RS5_PSEPK 611 30S ribosomal protein S5 OS=Pseudomonas putida (strain KT2440) GN=rpsE PE=3 SV=1

+18

Accession Score Description
1 Q88R99_PSEPK 608 Porin E OS=Pseudomonas putida (strain KT2440) GN=oprE PE=4 SV=1

+19

Accession Score Description
1 CH60_PSEPK 589 60 kDa chaperonin OS=Pseudomonas putida (strain KT2440) GN=groL PE=3 SV=1

+20

Accession Score Description
1 Q88PD7_PSEPK 581 Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_0913 PE=4 SV=1
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