MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita1 |
| MS data file | : | PRT1270_T-BRSC_1_20250714114344.mgf |
| Database | : | P-putida 20180924 (5,556 sequences; 1,892,173 residues) |
| Timestamp | : | 12 Aug 2025 at 22:47:09 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,938 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle () in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
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141| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88F96_PSEPK | 99 | Electron transfer flavoprotein, beta subunit OS=Pseudomonas putida (strain KT2440) GN=PP_4202 PE=4 SV=1 |
142| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88IU6_PSEPK | 97 | Peptidyl-prolyl cis-trans isomerase OS=Pseudomonas putida (strain KT2440) GN=ppiB PE=3 SV=1 |
143| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88D83_PSEPK | 96 | Carbamoyltransferase, NodU family OS=Pseudomonas putida (strain KT2440) GN=PP_4944 PE=4 SV=1 |
144| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88FA5_PSEPK | 95 | Succinate dehydrogenase, cytochrome b556 subunit OS=Pseudomonas putida (strain KT2440) GN=sdhC PE=4 SV=1 |
145| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL7_PSEPK | 95 | 50S ribosomal protein L7/L12 OS=Pseudomonas putida (strain KT2440) GN=rplL PE=3 SV=2 |
146| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | AROB_PSEPK | 93 | 3-dehydroquinate synthase OS=Pseudomonas putida (strain KT2440) GN=aroB PE=3 SV=1 |
147| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88FA6_PSEPK | 93 | Succinate dehydrogenase, hydrophobic membrane anchor protein OS=Pseudomonas putida (strain KT2440) GN=sdhD PE=4 SV=1 |
148| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88NS0_PSEPK | 93 | Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_1135 PE=4 SV=1 |
149| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88F35_PSEPK | 92 | Coproporphyrinogen-III oxidase OS=Pseudomonas putida (strain KT2440) GN=hemN PE=3 SV=1 |
150| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL23_PSEPK | 92 | 50S ribosomal protein L23 OS=Pseudomonas putida (strain KT2440) GN=rplW PE=3 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 150.1 | RL23_PSEPK |
92 | 10893 | 5 (3) | 5 (3) | 0.82 | |
| 50S ribosomal protein L23 OS=Pseudomonas putida (strain KT2440) GN=rplW PE=3 SV=1 | |||||||
5 peptide matches (5 non-duplicate, 0 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 537 |
366.2195 | 730.4244 | 730.4225 | 2.69 | 1 | 18 | 0.1 | 1Score > 21 indicates identity |
U | K.ATVLAEK.K | |
| 1108 |
488.7798 | 975.5450 | 975.5389 | 6.28 | 1 | 41 | 0.00027 | 1Score > 18 indicates identity |
U | K.AVEGLFNVK.V | |
| 1312 |
360.2144 | 1077.6214 | 1077.6182 | 2.91 | 1 | 29 | 0.0026 | 1Score > 15 indicates identity |
U | K.VLLGPHVSEK.A | |
| 1706 |
629.8570 | 1257.6994 | 1257.6929 | 5.23 | 1 | 57 | 1e-005 | 1Score > 19 indicates identity |
U | K.VENVSTVNVLGK.T | |
| 2952 |
932.4613 | 1862.9080 | 1862.8898 | 9.80 | 0 | 19 | 0.068 | 1Score > 20 indicates identity |
U | K.AIVSLQPGQDLDFSSSAE.- |
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