MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 12 Aug 2025 at 22:47:09 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 11–20 (out of 313)

Page: Previous 1 2 3 4 5 6 7 … 32 Next 

+11

Accession Score Description
Family member distances as a dendrogram 1 Q88NK1_PSEPK 752 Porin D OS=Pseudomonas putida (strain KT2440) GN=oprD PE=4 SV=1
3 Q88MB8_PSEPK 45 GTP pyrophosphokinase OS=Pseudomonas putida (strain KT2440) GN=relA PE=3 SV=1
2 Q88R66_PSEPK 273 Outer membrane protein OprE3 OS=Pseudomonas putida (strain KT2440) GN=oprQ PE=4 SV=1

+12

Accession Score Description
1 Q88FA9_PSEPK 748 2-oxoglutarate dehydrogenase, E1 component OS=Pseudomonas putida (strain KT2440) GN=kgdA PE=4 SV=1

+13

Accession Score Description
1 RPOC_PSEPK 704 DNA-directed RNA polymerase subunit beta' OS=Pseudomonas putida (strain KT2440) GN=rpoC PE=3 SV=1

+14

Accession Score Description
1 RL3_PSEPK 676 50S ribosomal protein L3 OS=Pseudomonas putida (strain KT2440) GN=rplC PE=3 SV=2

-15

Accession Score Description
1 Q88FB1_PSEPK 674 Dihydrolipoyl dehydrogenase OS=Pseudomonas putida (strain KT2440) GN=lpdG PE=4 SV=1
Score Mass Matches Sequences emPAI
15.1 Q88FB1_PSEPK 674 50109 28 (23) 18 (15) 1.45
Dihydrolipoyl dehydrogenase OS=Pseudomonas putida (strain KT2440) GN=lpdG PE=4 SV=1

-28 peptide matches (25 non-duplicate, 3 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
212   487.3255 486.3182 486.3166 3.39 0 28 0.026 +1Score > 24 indicates identity U K.AGIVK.N
219   501.3408 500.3335 500.3322 2.62 0 30 0.0055 +1Score > 20 indicates identity U K.LLAGK.K
322   308.6876 615.3606 615.3592 2.42 0 18 0.24 +2Score > 24 indicates identity U K.VIADAK.T
333   312.1740 622.3334 622.3326 1.32 0 20 0.021 +1Score > 20 indicates identity
Score > 16 indicates homology		 U K.ITFDK.L
346   315.2218 628.4290 628.4272 2.99 1 4 0.52 +1Score > 14 indicates identity U K.LLAGKK.V
414   673.4001 672.3928 672.3806 18.1 0 28 0.017 +1Score > 23 indicates identity U K.QGLDIK.L
467   350.7220 699.4294 699.4279 2.21 0 24 0.033 +1Score > 22 indicates identity U K.AAQLGLK.T
468   700.4371 699.4298 699.4279 2.75 0 45 0.00029 +1Score > 22 indicates identity U K.AAQLGLK.T
524   727.4844 726.4771 726.4752 2.64 0 31 0.0019 +1Score > 16 indicates identity U K.LIVAVGR.R
525   364.2462 726.4778 726.4752 3.64 0 25 0.0078 +1Score > 16 indicates identity U K.LIVAVGR.R
949   460.2493 918.4840 918.4811 3.25 0 35 0.0025 +1Score > 21 indicates identity U K.ALLDSSWK.Y
985   465.7681 929.5216 929.5070 15.8 1 5 2 +2Score > 21 indicates identity
Score > 20 indicates homology		 U R.LGAEVTVLE.A
1393   1120.6439 1119.6366 1119.6288 6.97 0 88 3.9e-009 +1Score > 16 indicates identity U K.NLTGGVATLFK.A
1394   560.8265 1119.6384 1119.6288 8.60 0 47 3.9e-005 +1Score > 15 indicates identity U K.NLTGGVATLFK.A
1401   1121.6271 1120.6198 1120.6128 6.25 0 4 1.4 +2Score > 18 indicates identity U K.NLTGGVATLFK.A + Deamidated (NQ)
1402 +1 561.3199 1120.6252 1120.6128 11.1 0 24 0.014 +2Score > 17 indicates identity U K.NLTGGVATLFK.A + Deamidated (NQ)
1515   390.2065 1167.5977 1167.5996 -1.69 0 18 0.088 +1Score > 20 indicates identity U K.ANGVTSIQGHGK.L
1516   584.8085 1167.6024 1167.5996 2.40 0 67 6.7e-007 +1Score > 20 indicates identity
Score > 18 indicates homology		 U K.ANGVTSIQGHGK.L
1549   591.2859 1180.5572 1180.5547 2.19 0 43 0.00033 +1Score > 21 indicates identity U R.AMAANDTGGFVK.V
2707   866.9929 1731.9712 1731.9560 8.82 0 38 0.00035 +1Score > 16 indicates identity U K.FDVVVIGAGPGGYVAAIK.A
2962   934.0498 1866.0850 1866.0727 6.60 1 81 7.4e-009 +1Score > 13 indicates identity U R.LGVIGAGVIGLELGSVWAR.L
2963   623.0397 1866.0973 1866.0727 13.2 1 59 1.3e-006 +1Score > 13 indicates identity U R.LGVIGAGVIGLELGSVWAR.L
3241 +2 682.3595 2044.0567 2044.0437 6.36 1 85 1.5e-008 +1Score > 19 indicates identity U R.RPVTTDLLASDSGVTIDER.G
3320   697.4011 2089.1815 2089.1572 11.6 1 63 6.4e-007 +1Score > 14 indicates identity U M.TQKFDVVVIGAGPGGYVAAIK.A
4039   882.4583 2644.3531 2644.3359 6.49 1 47 6.1e-005 +1Score > 17 indicates identity U K.AQMNYDLIPSVIYTHPEIAWVGK.T

2 subsets and intersections (7 subset proteins in total)

Score Mass Subset of
ECOT_PSEPK 30 0 15.1
description
4 samesets of ECOT_PSEPK
Q88F18_PSEPK 30 0
description
Q88CJ9_PSEPK 30 40156
Putrescine ABC transporter, periplasmic putrescine-binding protein OS=Pseudomonas putida (strain KT2440) GN=potF-2 PE=4 SV=1
Q88EP9_PSEPK 30 0
description
Q88MT2_PSEPK 30 41987
Polyamine ABC transporter, periplasmic polyamine-binding protein OS=Pseudomonas putida (strain KT2440) GN=PP_1486 PE=4 SV=1
Q88L97_PSEPK 28 0 15.1
description
1 sameset of Q88L97_PSEPK
SYY_PSEPK 28 44317
Tyrosine--tRNA ligase OS=Pseudomonas putida (strain KT2440) GN=tyrS PE=3 SV=1

+16

Accession Score Description
1 RS2_PSEPK 613 30S ribosomal protein S2 OS=Pseudomonas putida (strain KT2440) GN=rpsB PE=3 SV=1

+17

Accession Score Description
1 RS5_PSEPK 611 30S ribosomal protein S5 OS=Pseudomonas putida (strain KT2440) GN=rpsE PE=3 SV=1

+18

Accession Score Description
1 Q88R99_PSEPK 608 Porin E OS=Pseudomonas putida (strain KT2440) GN=oprE PE=4 SV=1

+19

Accession Score Description
1 CH60_PSEPK 589 60 kDa chaperonin OS=Pseudomonas putida (strain KT2440) GN=groL PE=3 SV=1

+20

Accession Score Description
1 Q88PD7_PSEPK 581 Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_0913 PE=4 SV=1
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