MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita1 |
| MS data file | : | PRT1270_T-BRSC_1_20250714114344.mgf |
| Database | : | P-putida 20180924 (5,556 sequences; 1,892,173 residues) |
| Timestamp | : | 12 Aug 2025 at 22:47:09 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,938 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
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11| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88NK1_PSEPK | 752 | Porin D OS=Pseudomonas putida (strain KT2440) GN=oprD PE=4 SV=1 | |
| 3 | Q88MB8_PSEPK | 45 | GTP pyrophosphokinase OS=Pseudomonas putida (strain KT2440) GN=relA PE=3 SV=1 | |
| 2 | Q88R66_PSEPK | 273 | Outer membrane protein OprE3 OS=Pseudomonas putida (strain KT2440) GN=oprQ PE=4 SV=1 | |
12| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88FA9_PSEPK | 748 | 2-oxoglutarate dehydrogenase, E1 component OS=Pseudomonas putida (strain KT2440) GN=kgdA PE=4 SV=1 |
13| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RPOC_PSEPK | 704 | DNA-directed RNA polymerase subunit beta' OS=Pseudomonas putida (strain KT2440) GN=rpoC PE=3 SV=1 |
14| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL3_PSEPK | 676 | 50S ribosomal protein L3 OS=Pseudomonas putida (strain KT2440) GN=rplC PE=3 SV=2 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 14.1 | RL3_PSEPK |
676 | 22703 | 27 (23) | 11 (9) | 4.79 | |
| 50S ribosomal protein L3 OS=Pseudomonas putida (strain KT2440) GN=rplC PE=3 SV=2 | |||||||
27 peptide matches (21 non-duplicate, 6 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
| 330 |
1 |
311.6823 | 621.3500 | 621.3486 | 2.30 | 0 | 16 | 0.035 | 1Score > 13 indicates identity |
U | R.VTQFK.T |
| 332 |
622.3580 | 621.3507 | 621.3486 | 3.39 | 0 | 20 | 0.011 | 1Score > 13 indicates identity |
U | R.VTQFK.T | |
| 458 |
693.3949 | 692.3876 | 692.3857 | 2.74 | 0 | 61 | 2.8e-006 | 1Score > 18 indicates identity |
U | K.GFAGTIK.R | |
| 459 |
347.2014 | 692.3882 | 692.3857 | 3.64 | 0 | 22 | 0.024 | 1Score > 18 indicates identity |
U | K.GFAGTIK.R | |
| 496 |
1 |
357.2516 | 712.4886 | 712.4847 | 5.57 | 0 | 30 | 0.0011 | 1Score > 13 indicates identity |
U | R.NLLLIK.G |
| 497 |
713.4960 | 712.4887 | 712.4847 | 5.68 | 0 | 35 | 0.00031 | 1Score > 13 indicates identity |
U | R.NLLLIK.G | |
| 501 |
715.4481 | 714.4408 | 714.4388 | 2.79 | 0 | 35 | 0.0029 | 1Score > 22 indicates identity |
U | M.TIGVIGR.K | |
| 502 |
358.2283 | 714.4420 | 714.4388 | 4.51 | 0 | 26 | 0.015 | 2Score > 20 indicates identity |
U | M.TIGVIGR.K | |
| 662 |
793.4011 | 792.3938 | 792.3919 | 2.46 | 1 | 18 | 0.077 | 1Score > 19 indicates identity |
U | R.GVWEFR.L | |
| 663 |
397.2043 | 792.3940 | 792.3919 | 2.74 | 1 | 12 | 0.34 | 1Score > 19 indicates identity |
U | R.GVWEFR.L | |
| 786 |
425.2464 | 848.4782 | 848.4868 | -10.1 | 1 | 1 | 1.4 | 2Score > 20 indicates identity Score > 15 indicates homology |
U | K.GFAGTIKR.W | |
| 1069 |
958.5339 | 957.5266 | 957.5244 | 2.37 | 1 | 54 | 2.9e-005 | 1Score > 22 indicates identity |
U | R.AVQVTVGER.R | |
| 1070 |
479.7712 | 957.5278 | 957.5244 | 3.65 | 1 | 47 | 4.2e-005 | 1Score > 22 indicates identity Score > 16 indicates homology |
U | R.AVQVTVGER.R | |
| 1351 |
367.5343 | 1099.5811 | 1099.5774 | 3.30 | 0 | 31 | 0.0063 | 1Score > 22 indicates identity |
U | R.VTAAQAGHFAK.A | |
| 1352 |
550.7981 | 1099.5816 | 1099.5774 | 3.83 | 0 | 75 | 2.8e-007 | 1Score > 22 indicates identity |
U | R.VTAAQAGHFAK.A | |
| 1354 |
551.2931 | 1100.5716 | 1100.5614 | 9.27 | 0 | 54 | 2e-005 | 1Score > 22 indicates identity Score > 19 indicates homology |
U | R.VTAAQAGHFAK.A + Deamidated (NQ) | |
| 1418 |
1 |
565.3352 | 1128.6558 | 1128.6503 | 4.93 | 1 | 53 | 1.3e-005 | 1Score > 17 indicates identity |
U | R.VTVQSLEVVR.V |
| 2506 |
825.4725 | 1648.9304 | 1648.9261 | 2.65 | 0 | 63 | 5.6e-007 | 1Score > 13 indicates identity |
U | K.GAVPGATGGDVVVRPAVK.A | |
| 2507 |
1 |
550.6510 | 1648.9312 | 1648.9261 | 3.08 | 0 | 49 | 1.4e-005 | 1Score > 13 indicates identity |
U | K.GAVPGATGGDVVVRPAVK.A |
| 2762 |
2 |
882.4694 | 1762.9242 | 1762.9101 | 8.00 | 1 | 89 | 6.5e-009 | 1Score > 19 indicates identity |
U | K.AELFTAGQLVDVTGQSK.G |
| 2766 |
588.9826 | 1763.9260 | 1763.8942 | 18.0 | 1 | 47 | 9.9e-005 | 1Score > 19 indicates identity |
U | K.AELFTAGQLVDVTGQSK.G + Deamidated (NQ) |
15| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88FB1_PSEPK | 674 | Dihydrolipoyl dehydrogenase OS=Pseudomonas putida (strain KT2440) GN=lpdG PE=4 SV=1 |
16| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RS2_PSEPK | 613 | 30S ribosomal protein S2 OS=Pseudomonas putida (strain KT2440) GN=rpsB PE=3 SV=1 |
17| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RS5_PSEPK | 611 | 30S ribosomal protein S5 OS=Pseudomonas putida (strain KT2440) GN=rpsE PE=3 SV=1 |
18| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88R99_PSEPK | 608 | Porin E OS=Pseudomonas putida (strain KT2440) GN=oprE PE=4 SV=1 |
19| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | CH60_PSEPK | 589 | 60 kDa chaperonin OS=Pseudomonas putida (strain KT2440) GN=groL PE=3 SV=1 |
20| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88PD7_PSEPK | 581 | Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_0913 PE=4 SV=1 |
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