MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 12 Aug 2025 at 22:47:09 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 131–140 (out of 313)


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+131

Accession Score Description
1 Q88N39_PSEPK 107 Beta-ketoadipyl CoA thiolase PcaF OS=Pseudomonas putida (strain KT2440) GN=pcaF PE=3 SV=1

+132

Accession Score Description
1 Q88MC7_PSEPK 107 Thioredoxin, putative OS=Pseudomonas putida (strain KT2440) GN=PP_1646 PE=4 SV=1

+133

Accession Score Description
1 Q88R47_PSEPK 107 Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_0287 PE=4 SV=1

+134

Accession Score Description
1 Q88P44_PSEPK 106 Glyceraldehyde-3-phosphate dehydrogenase OS=Pseudomonas putida (strain KT2440) GN=gap-1 PE=3 SV=1

-135

Accession Score Description
1 ATPE_PSEPK 104 ATP synthase epsilon chain OS=Pseudomonas putida (strain KT2440) GN=atpC PE=3 SV=1
Score Mass Matches Sequences emPAI
135.1 ATPE_PSEPK 104 14585 5 (3) 3 (3) 0.57
ATP synthase epsilon chain OS=Pseudomonas putida (strain KT2440) GN=atpC PE=3 SV=1

-5 peptide matches (4 non-duplicate, 1 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
1021 +1 471.7745 941.5344 941.5294 5.37 1 63 2.7e-006 +1Score > 20 indicates identity U R.LAEAAAQLR.T
1026   472.2664 942.5182 942.5134 5.15 1 23 0.042 +1Score > 22 indicates identity U K.AAENALNLK.G
1029   945.4904 944.4831 944.4814 1.81 1 11 0.89 +6Score > 23 indicates identity U K.AAENALNLK.G + 2 Deamidated (NQ)
1559   592.7721 1183.5296 1183.5258 3.26 0 58 6e-006 +1Score > 18 indicates identity U K.GADFDYGAAAAR.L

+136

Accession Score Description
1 GUAA_PSEPK 103 GMP synthase [glutamine-hydrolyzing] OS=Pseudomonas putida (strain KT2440) GN=guaA PE=3 SV=1

+137

Accession Score Description
1 NUOI_PSEPK 103 NADH-quinone oxidoreductase subunit I OS=Pseudomonas putida (strain KT2440) GN=nuoI PE=3 SV=1

+138

Accession Score Description
1 Q88DB6_PSEPK 103 Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_4911 PE=4 SV=1

+139

Accession Score Description
1 Q88PJ5_PSEPK 100 Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_0855 PE=4 SV=1

+140

Accession Score Description
1 RL17_PSEPK 100 50S ribosomal protein L17 OS=Pseudomonas putida (strain KT2440) GN=rplQ PE=3 SV=1
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