MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita1 |
| MS data file | : | PRT1270_T-BRSC_1_20250714114344.mgf |
| Database | : | P-putida 20180924 (5,556 sequences; 1,892,173 residues) |
| Timestamp | : | 12 Aug 2025 at 22:47:09 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,938 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle () in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
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131| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88N39_PSEPK | 107 | Beta-ketoadipyl CoA thiolase PcaF OS=Pseudomonas putida (strain KT2440) GN=pcaF PE=3 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 131.1 | Q88N39_PSEPK |
107 | 42053 | 6 (5) | 5 (4) | 0.30 | |
| Beta-ketoadipyl CoA thiolase PcaF OS=Pseudomonas putida (strain KT2440) GN=pcaF PE=3 SV=1 | |||||||
6 peptide matches (6 non-duplicate, 0 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 275 |
583.3983 | 582.3910 | 582.3853 | 9.78 | 0 | 2 | 0.62 | 4Score > 13 indicates identity |
U | E.IVPVR.I | |
| 1178 |
506.7883 | 1011.5620 | 1011.5600 | 1.98 | 0 | 47 | 6.4e-005 | 1Score > 18 indicates identity |
U | R.ADDLAAVPLK.A | |
| 1333 |
545.7815 | 1089.5484 | 1089.5455 | 2.73 | 1 | 41 | 0.00074 | 1Score > 22 indicates identity |
U | K.LEDTTIGWR.F | |
| 1715 |
422.2616 | 1263.7630 | 1263.7551 | 6.27 | 1 | 47 | 1.8e-005 | 1Score > 13 indicates identity |
U | R.LVLTALHQLEK.S | |
| 3096 |
983.5740 | 1965.1334 | 1965.0968 | 18.6 | 1 | 19 | 0.011 | 1Score > 13 indicates identity |
U | R.MALLLAGLPESIPGVTLNR.L + Deamidated (NQ) | |
| 3097 |
656.0524 | 1965.1354 | 1965.0968 | 19.6 | 1 | 15 | 0.029 | 1Score > 13 indicates identity |
U | R.MALLLAGLPESIPGVTLNR.L + Deamidated (NQ) |
132| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88MC7_PSEPK | 107 | Thioredoxin, putative OS=Pseudomonas putida (strain KT2440) GN=PP_1646 PE=4 SV=1 |
133| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88R47_PSEPK | 107 | Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_0287 PE=4 SV=1 |
134| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88P44_PSEPK | 106 | Glyceraldehyde-3-phosphate dehydrogenase OS=Pseudomonas putida (strain KT2440) GN=gap-1 PE=3 SV=1 |
135| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ATPE_PSEPK | 104 | ATP synthase epsilon chain OS=Pseudomonas putida (strain KT2440) GN=atpC PE=3 SV=1 |
136| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | GUAA_PSEPK | 103 | GMP synthase [glutamine-hydrolyzing] OS=Pseudomonas putida (strain KT2440) GN=guaA PE=3 SV=1 |
137| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | NUOI_PSEPK | 103 | NADH-quinone oxidoreductase subunit I OS=Pseudomonas putida (strain KT2440) GN=nuoI PE=3 SV=1 |
138| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88DB6_PSEPK | 103 | Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_4911 PE=4 SV=1 |
139| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88PJ5_PSEPK | 100 | Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_0855 PE=4 SV=1 |
140| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL17_PSEPK | 100 | 50S ribosomal protein L17 OS=Pseudomonas putida (strain KT2440) GN=rplQ PE=3 SV=1 |
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