MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 12 Aug 2025 at 22:47:09 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 11–20 (out of 313)

Page: Previous 1 2 3 4 5 6 7 … 32 Next 

+11

Accession Score Description
Family member distances as a dendrogram 1 Q88NK1_PSEPK 752 Porin D OS=Pseudomonas putida (strain KT2440) GN=oprD PE=4 SV=1
3 Q88MB8_PSEPK 45 GTP pyrophosphokinase OS=Pseudomonas putida (strain KT2440) GN=relA PE=3 SV=1
2 Q88R66_PSEPK 273 Outer membrane protein OprE3 OS=Pseudomonas putida (strain KT2440) GN=oprQ PE=4 SV=1

+12

Accession Score Description
1 Q88FA9_PSEPK 748 2-oxoglutarate dehydrogenase, E1 component OS=Pseudomonas putida (strain KT2440) GN=kgdA PE=4 SV=1

-13

Accession Score Description
1 RPOC_PSEPK 704 DNA-directed RNA polymerase subunit beta' OS=Pseudomonas putida (strain KT2440) GN=rpoC PE=3 SV=1
Score Mass Matches Sequences emPAI
13.1 RPOC_PSEPK 704 155386 33 (22) 29 (22) 0.38
DNA-directed RNA polymerase subunit beta' OS=Pseudomonas putida (strain KT2440) GN=rpoC PE=3 SV=1

-33 peptide matches (32 non-duplicate, 1 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
461   347.7042 693.3938 693.3922 2.38 0 19 0.036 +1Score > 17 indicates identity U R.APTLHR.L
478 +1 352.2034 702.3922 702.3912 1.51 1 15 0.28 +3Score > 22 indicates identity U R.INETVK.E
482   703.4376 702.4303 702.4276 3.90 0 7 0.29 +4Score > 15 indicates identity U R.GLATTIK.A
483   352.2225 702.4304 702.4276 4.08 0 17 0.03 +1Score > 15 indicates identity U R.GLATTIK.A
563   372.2564 742.4982 742.4953 4.01 0 31 0.00073 +1Score > 13 indicates identity U R.VLLGITK.A
613   386.7264 771.4382 771.4239 18.6 1 10 1.2 +6Score > 24 indicates identity U K.ENVVVGR.L
664   397.2092 792.4038 792.4051 -1.61 0 5 2.1 +5Score > 21 indicates identity U K.SLADMIK.G + Oxidation (M)
738   415.2443 828.4740 828.4817 -9.26 1 12 0.73 +10Score > 23 indicates identity U R.QNLLGKR.V + Deamidated (NQ)
785   849.4850 848.4777 848.4868 -10.7 0 6 1.3 +6Score > 20 indicates identity U E.HPVLLNR.A + Deamidated (NQ)
828   430.7469 859.4792 859.4763 3.37 0 22 0.056 +1Score > 22 indicates identity U R.IVDTTVGR.A
830   430.7499 859.4852 859.4803 5.70 0 37 0.0014 +1Score > 20 indicates identity U K.VIDLWSK.A
842   436.2866 870.5586 870.5538 5.53 0 54 3.6e-006 +1Score > 13 indicates identity U R.LLGVSALAK.Y
948   458.7777 915.5408 915.5389 2.12 1 54 1.3e-005 +1Score > 18 indicates identity U R.KGLADTALK.T
1000   470.2334 938.4522 938.4498 2.63 1 23 0.024 +1Score > 19 indicates identity U R.SWSFGEVK.K
1125   494.7892 987.5638 987.5601 3.84 1 56 1.1e-005 +1Score > 19 indicates identity U R.VLTEAAVTGK.R
1152   501.2749 1000.5352 1000.5302 5.09 0 36 0.00089 +1Score > 22 indicates identity
Score > 18 indicates homology		 U R.ADGNLVAVSR.S
1166   503.2654 1004.5162 1004.5138 2.40 0 59 3.2e-006 +1Score > 21 indicates identity
Score > 17 indicates homology		 U R.TSAADSVQVK.N
1169   503.7581 1005.5016 1005.4978 3.78 0 15 0.21 +2Score > 21 indicates identity U R.TSAADSVQVK.N + Deamidated (NQ)
1184   339.5147 1015.5223 1015.5199 2.31 0 32 0.0025 +1Score > 22 indicates identity
Score > 19 indicates homology		 U R.TFHIGGAASR.T
1237   521.8186 1041.6226 1041.6183 4.22 0 30 0.0035 +1Score > 18 indicates identity U R.SVITVGPTLR.L
1265   530.2897 1058.5648 1058.5608 3.85 1 38 0.00035 +1Score > 22 indicates identity
Score > 16 indicates homology		 U R.IIGNATDEVK.E
1574   596.3060 1190.5974 1190.5932 3.61 1 48 0.00012 +1Score > 22 indicates identity U R.VFADLQEVDR.V
1806   657.8246 1313.6346 1313.6252 7.22 0 69 6.8e-007 +1Score > 20 indicates identity U R.FATSDLNDLYR.R
1830   662.8994 1323.7842 1323.7762 6.09 0 63 4.9e-007 +1Score > 13 indicates identity U R.LLDLSAPDIIVR.N
1849   446.6024 1336.7854 1336.7755 7.42 1 52 8.5e-006 +1Score > 13 indicates identity U R.YKLPYGAVISVK.E
2006   706.3684 1410.7222 1410.7143 5.63 1 82 3.3e-008 +1Score > 21 indicates identity
Score > 20 indicates homology		 U K.YIVNEIQDVYR.L
2146   495.6019 1483.7839 1483.7783 3.75 1 70 5.9e-007 +1Score > 20 indicates identity U R.LIPAGTGLAYHSER.K
2230   757.4431 1512.8716 1512.8552 10.9 1 74 5.3e-008 +1Score > 14 indicates identity U R.LGIQAFEPVLIEGK.A
2332   393.9612 1571.8157 1571.8096 3.86 1 12 0.39 +1Score > 20 indicates identity U K.WDPHTHPIVTELK.G
2642   569.6180 1705.8322 1705.8271 2.95 1 68 5.1e-007 +1Score > 21 indicates identity
Score > 18 indicates homology		 U R.GEVISDGPSDPHDILR.L
2803   889.4735 1776.9324 1776.9159 9.33 1 1 3.5 +2Score > 19 indicates identity U R.EGLSVLQYFISTHGAR.K
2804   593.3192 1776.9358 1776.9159 11.2 1 52 2.6e-005 +1Score > 19 indicates identity U R.EGLSVLQYFISTHGAR.K

1 subset or intersection (1 subset protein in total)

Score Mass Subset of
ARGD_PSEPK 17 43862 13.1
Acetylornithine aminotransferase OS=Pseudomonas putida (strain KT2440) GN=argD PE=3 SV=1

+14

Accession Score Description
1 RL3_PSEPK 676 50S ribosomal protein L3 OS=Pseudomonas putida (strain KT2440) GN=rplC PE=3 SV=2

+15

Accession Score Description
1 Q88FB1_PSEPK 674 Dihydrolipoyl dehydrogenase OS=Pseudomonas putida (strain KT2440) GN=lpdG PE=4 SV=1

+16

Accession Score Description
1 RS2_PSEPK 613 30S ribosomal protein S2 OS=Pseudomonas putida (strain KT2440) GN=rpsB PE=3 SV=1

+17

Accession Score Description
1 RS5_PSEPK 611 30S ribosomal protein S5 OS=Pseudomonas putida (strain KT2440) GN=rpsE PE=3 SV=1

+18

Accession Score Description
1 Q88R99_PSEPK 608 Porin E OS=Pseudomonas putida (strain KT2440) GN=oprE PE=4 SV=1

+19

Accession Score Description
1 CH60_PSEPK 589 60 kDa chaperonin OS=Pseudomonas putida (strain KT2440) GN=groL PE=3 SV=1

+20

Accession Score Description
1 Q88PD7_PSEPK 581 Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_0913 PE=4 SV=1
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