MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita1 |
| MS data file | : | PRT1270_T-BRSC_1_20250714114344.mgf |
| Database | : | P-putida 20180924 (5,556 sequences; 1,892,173 residues) |
| Timestamp | : | 12 Aug 2025 at 22:47:09 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,938 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
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121| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | PUR4_PSEPK | 119 | Phosphoribosylformylglycinamidine synthase OS=Pseudomonas putida (strain KT2440) GN=purL PE=3 SV=1 |
122| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88GH0_PSEPK | 118 | Beta-ketothiolase OS=Pseudomonas putida (strain KT2440) GN=PP_3754 PE=3 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 122.1 | Q88GH0_PSEPK |
118 | 41133 | 5 (3) | 5 (3) | 0.18 | |
| Beta-ketothiolase OS=Pseudomonas putida (strain KT2440) GN=PP_3754 PE=3 SV=1 | |||||||
5 peptide matches (5 non-duplicate, 0 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 309 |
303.1460 | 604.2774 | 604.2704 | 11.6 | 0 | 7 | 1.2 | 2Score > 23 indicates identity Score > 20 indicates homology |
R.ADVTAE.Q | ||
| 1110 |
489.2757 | 976.5368 | 976.5229 | 14.2 | 0 | 2 | 3.4 | 4Score > 20 indicates identity |
U | R.FASQIVALE.L | |
| 1179 |
507.7924 | 1013.5702 | 1013.5658 | 4.39 | 0 | 25 | 0.024 | 1Score > 21 indicates identity |
U | R.GPYLLPQAR.W | |
| 1625 |
609.8630 | 1217.7114 | 1217.7019 | 7.80 | 1 | 67 | 3.5e-007 | 1Score > 15 indicates identity |
U | R.FASQIVALELK.T | |
| 1854 |
671.3788 | 1340.7430 | 1340.7300 | 9.76 | 0 | 63 | 2.6e-006 | 1Score > 20 indicates identity |
U | K.DLPLADLASAVTR.A |
123| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88N95_PSEPK | 118 | Ubiquinol-cytochrome c reductase iron-sulfur subunit OS=Pseudomonas putida (strain KT2440) GN=petA PE=4 SV=1 |
124| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ILVC_PSEPK | 115 | Ketol-acid reductoisomerase OS=Pseudomonas putida (strain KT2440) GN=ilvC PE=3 SV=1 |
125| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88PB7_PSEPK | 111 | Rod shape-determining protein MreB OS=Pseudomonas putida (strain KT2440) GN=mreB PE=4 SV=1 |
126| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88PN6_PSEPK | 111 | Cytochrome o ubiquinol oxidase, subunit I OS=Pseudomonas putida (strain KT2440) GN=cyoB PE=3 SV=1 |
127| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88LG1_PSEPK | 111 | Aromatic-amino-acid aminotransferase OS=Pseudomonas putida (strain KT2440) GN=tyrB-1 PE=3 SV=1 |
128| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ARCA_PSEPK | 109 | Arginine deiminase OS=Pseudomonas putida (strain KT2440) GN=arcA PE=3 SV=1 |
129| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88N37_PSEPK | 109 | 3-carboxy-cis,cis-muconate cycloisomerase OS=Pseudomonas putida (strain KT2440) GN=pcaB PE=1 SV=1 |
130| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | CARB_PSEPK | 108 | Carbamoyl-phosphate synthase large chain OS=Pseudomonas putida (strain KT2440) GN=carB PE=3 SV=2 |
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