MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita1 |
| MS data file | : | PRT1270_T-BRSC_1_20250714114344.mgf |
| Database | : | P-putida 20180924 (5,556 sequences; 1,892,173 residues) |
| Timestamp | : | 12 Aug 2025 at 22:47:09 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,938 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
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121| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | PUR4_PSEPK | 119 | Phosphoribosylformylglycinamidine synthase OS=Pseudomonas putida (strain KT2440) GN=purL PE=3 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 121.1 | PUR4_PSEPK |
119 | 141586 | 10 (5) | 10 (5) | 0.08 | |
| Phosphoribosylformylglycinamidine synthase OS=Pseudomonas putida (strain KT2440) GN=purL PE=3 SV=1 | |||||||
10 peptide matches (10 non-duplicate, 0 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 482 |
703.4376 | 702.4303 | 702.4276 | 3.92 | 1 | 10 | 0.16 | 2Score > 15 indicates identity |
U | E.KLSDIK.L | |
| 735 |
414.7526 | 827.4906 | 827.4865 | 5.02 | 0 | 10 | 0.83 | 1Score > 22 indicates identity |
U | K.TLTPQLR.M | |
| 771 |
422.2793 | 842.5440 | 842.5378 | 7.40 | 0 | 31 | 0.00078 | 1Score > 13 indicates identity |
U | R.LFLVVPR.L | |
| 1073 |
480.7735 | 959.5324 | 959.5175 | 15.6 | 1 | 1 | 3.5 | 3Score > 19 indicates identity |
U | K.LLEQLSQK.V + 2 Deamidated (NQ) | |
| 1195 |
511.2923 | 1020.5700 | 1020.5604 | 9.46 | 0 | 34 | 0.0013 | 1Score > 17 indicates identity |
U | K.SFLITIGDR.T | |
| 1421 |
565.7740 | 1129.5334 | 1129.5193 | 12.6 | 1 | 30 | 0.0037 | 1Score > 21 indicates identity Score > 18 indicates homology |
U | R.DAFQAFFER.T | |
| 1727 |
636.8400 | 1271.6654 | 1271.6470 | 14.5 | 0 | 14 | 0.35 | 1Score > 22 indicates identity |
U | K.DNASVIVGNVAGR.F + Deamidated (NQ) | |
| 1755 |
641.3650 | 1280.7154 | 1280.7088 | 5.17 | 0 | 55 | 8.8e-006 | 1Score > 17 indicates identity |
U | R.TPLALLDAPASGR.M | |
| 1915 |
684.8696 | 1367.7246 | 1367.7085 | 11.8 | 1 | 36 | 0.0011 | 1Score > 19 indicates identity |
U | R.YVLAVSAVDFER.F | |
| 2514 |
825.9281 | 1649.8416 | 1649.8301 | 7.01 | 0 | 0 | 2.4 | 2Score > 20 indicates identity Score > 16 indicates homology |
U | K.LGFDVNDDIAAPYIK.K |
122| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88GH0_PSEPK | 118 | Beta-ketothiolase OS=Pseudomonas putida (strain KT2440) GN=PP_3754 PE=3 SV=1 |
123| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88N95_PSEPK | 118 | Ubiquinol-cytochrome c reductase iron-sulfur subunit OS=Pseudomonas putida (strain KT2440) GN=petA PE=4 SV=1 |
124| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ILVC_PSEPK | 115 | Ketol-acid reductoisomerase OS=Pseudomonas putida (strain KT2440) GN=ilvC PE=3 SV=1 |
125| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88PB7_PSEPK | 111 | Rod shape-determining protein MreB OS=Pseudomonas putida (strain KT2440) GN=mreB PE=4 SV=1 |
126| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88PN6_PSEPK | 111 | Cytochrome o ubiquinol oxidase, subunit I OS=Pseudomonas putida (strain KT2440) GN=cyoB PE=3 SV=1 |
127| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88LG1_PSEPK | 111 | Aromatic-amino-acid aminotransferase OS=Pseudomonas putida (strain KT2440) GN=tyrB-1 PE=3 SV=1 |
128| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ARCA_PSEPK | 109 | Arginine deiminase OS=Pseudomonas putida (strain KT2440) GN=arcA PE=3 SV=1 |
129| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88N37_PSEPK | 109 | 3-carboxy-cis,cis-muconate cycloisomerase OS=Pseudomonas putida (strain KT2440) GN=pcaB PE=1 SV=1 |
130| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | CARB_PSEPK | 108 | Carbamoyl-phosphate synthase large chain OS=Pseudomonas putida (strain KT2440) GN=carB PE=3 SV=2 |
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