MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita1 |
| MS data file | : | PRT1270_T-BRSC_1_20250714114344.mgf |
| Database | : | P-putida 20180924 (5,556 sequences; 1,892,173 residues) |
| Timestamp | : | 12 Aug 2025 at 22:47:09 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,938 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
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111| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL22_PSEPK | 128 | 50S ribosomal protein L22 OS=Pseudomonas putida (strain KT2440) GN=rplV PE=3 SV=1 |
112| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88NA3_PSEPK | 128 | Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_1309 PE=4 SV=1 |
113| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88N87_PSEPK | 128 | Lipoprotein, putative OS=Pseudomonas putida (strain KT2440) GN=PP_1325 PE=4 SV=1 |
114| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88FI0_PSEPK | 127 | Isocitrate lyase OS=Pseudomonas putida (strain KT2440) GN=aceA PE=4 SV=1 |
115| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88F46_PSEPK | 126 | Cbb3-type cytochrome c oxidase subunit OS=Pseudomonas putida (strain KT2440) GN=ccoP-1 PE=3 SV=1 |
116| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88KX6_PSEPK | 126 | VacJ lipoprotein OS=Pseudomonas putida (strain KT2440) GN=vacJ PE=4 SV=1 |
117| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88HA4_PSEPK | 125 | Multidrug efflux RND transporter OS=Pseudomonas putida (strain KT2440) GN=PP_3456 PE=4 SV=1 |
118| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | KPRS_PSEPK | 125 | Ribose-phosphate pyrophosphokinase OS=Pseudomonas putida (strain KT2440) GN=prs PE=3 SV=1 |
119| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88L48_PSEPK | 124 | CmpX protein OS=Pseudomonas putida (strain KT2440) GN=cmpX PE=4 SV=1 |
120| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | DNAK_PSEPK | 119 | Chaperone protein DnaK OS=Pseudomonas putida (strain KT2440) GN=dnaK PE=3 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 120.1 | DNAK_PSEPK |
119 | 68873 | 9 (4) | 8 (4) | 0.14 | |
| Chaperone protein DnaK OS=Pseudomonas putida (strain KT2440) GN=dnaK PE=3 SV=1 | |||||||
9 peptide matches (9 non-duplicate, 0 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 289 |
589.3196 | 588.3123 | 588.3119 | 0.74 | 0 | 26 | 0.049 | 2Score > 25 indicates identity |
U | E.DVVQK.D + Deamidated (NQ) | |
| 617 |
775.4332 | 774.4259 | 774.4123 | 17.6 | 0 | 4 | 3.7 | 8Score > 22 indicates identity |
U | K.NTTIPTK.K + Deamidated (NQ) | |
| 658 |
790.3958 | 789.3885 | 789.3868 | 2.19 | 1 | 3 | 5.5 | 9Score > 22 indicates identity |
U | R.KQAAQNK.S + 3 Deamidated (NQ) | |
| 1027 |
472.7719 | 943.5292 | 943.5338 | -4.87 | 1 | 1 | 5.6 | 6Score > 21 indicates identity |
U | E.DVVQKDIK.L | |
| 1756 |
642.8351 | 1283.6556 | 1283.6795 | -18.6 | 1 | 1 | 4.1 | 4Score > 20 indicates identity |
U | E.MAPPQISAEVLK.K + Deamidated (NQ) | |
| 3405 |
1067.5671 | 2133.1196 | 2133.0954 | 11.4 | 1 | 38 | 0.00068 | 1Score > 18 indicates identity |
U | R.TTPSIVAYANDGEILVGQSAK.R | |
| 3673 |
765.4174 | 2293.2304 | 2293.1954 | 15.2 | 1 | 22 | 0.014 | 1Score > 16 indicates identity |
U | R.GVPQIEVTFDIDANGILHVGAK.D + Deamidated (NQ) | |
| 3674 |
765.4196 | 2293.2370 | 2293.1954 | 18.1 | 1 | 1 | 1.7 | 1Score > 16 indicates identity |
U | R.GVPQIEVTFDIDANGILHVGAK.D + Deamidated (NQ) | |
| 3929 |
835.0856 | 2502.2350 | 2502.1987 | 14.5 | 1 | 93 | 2.6e-009 | 1Score > 19 indicates identity |
U | K.SQVFSTADDNQSAVTIHVLQGER.K + Deamidated (NQ) |
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