MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita1 |
| MS data file | : | PRT1270_T-BRSC_1_20250714114344.mgf |
| Database | : | P-putida 20180924 (5,556 sequences; 1,892,173 residues) |
| Timestamp | : | 12 Aug 2025 at 22:47:09 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,938 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
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11| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88NK1_PSEPK | 752 | Porin D OS=Pseudomonas putida (strain KT2440) GN=oprD PE=4 SV=1 | |
| 3 | Q88MB8_PSEPK | 45 | GTP pyrophosphokinase OS=Pseudomonas putida (strain KT2440) GN=relA PE=3 SV=1 | |
| 2 | Q88R66_PSEPK | 273 | Outer membrane protein OprE3 OS=Pseudomonas putida (strain KT2440) GN=oprQ PE=4 SV=1 | |
12| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88FA9_PSEPK | 748 | 2-oxoglutarate dehydrogenase, E1 component OS=Pseudomonas putida (strain KT2440) GN=kgdA PE=4 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 12.1 | Q88FA9_PSEPK |
748 | 106911 | 40 (29) | 27 (22) | 0.73 | |
| 2-oxoglutarate dehydrogenase, E1 component OS=Pseudomonas putida (strain KT2440) GN=kgdA PE=4 SV=1 | |||||||
40 peptide matches (36 non-duplicate, 4 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
| 273 |
581.3325 | 580.3252 | 580.3221 | 5.47 | 0 | 33 | 0.00084 | 1Score > 14 indicates identity |
U | K.YLGTK.Y | |
| 305 |
603.3480 | 602.3407 | 602.3388 | 3.26 | 1 | 13 | 0.36 | 1Score > 22 indicates identity Score > 21 indicates homology |
U | R.LESVR.G | |
| 438 |
686.3508 | 685.3435 | 685.3435 | 0.0044 | 0 | 6 | 0.94 | 1Score > 18 indicates identity |
U | R.TYFQK.L | |
| 439 |
343.6797 | 685.3448 | 685.3435 | 1.94 | 0 | 4 | 1.3 | 1Score > 18 indicates identity |
U | R.TYFQK.L | |
| 462 |
348.1724 | 694.3302 | 694.3286 | 2.40 | 1 | 30 | 0.0058 | 1Score > 20 indicates identity |
U | K.IYEDR.Q | |
| 463 |
348.1724 | 694.3302 | 694.3286 | 2.40 | 1 | 17 | 0.12 | 1Score > 20 indicates identity |
K.IDEYR.N | ||
| 559 |
372.2235 | 742.4324 | 742.4337 | -1.73 | 1 | 35 | 0.0036 | 1Score > 23 indicates identity |
U | K.QVEVLR.L | |
| 598 |
382.2271 | 762.4396 | 762.4388 | 1.12 | 0 | 10 | 0.33 | 5Score > 18 indicates identity |
U | R.LIQAYR.M | |
| 599 |
382.7204 | 763.4262 | 763.4228 | 4.51 | 0 | 14 | 0.2 | 2Score > 20 indicates identity |
U | R.LIQAYR.M + Deamidated (NQ) | |
| 714 |
410.7335 | 819.4524 | 819.4491 | 4.15 | 0 | 34 | 0.002 | 1Score > 19 indicates identity |
U | R.AGDLFIGK.E | |
| 737 |
829.4808 | 828.4735 | 828.4705 | 3.66 | 1 | 45 | 0.00033 | 1Score > 23 indicates identity |
U | R.EDIAILR.I | |
| 738 |
415.2443 | 828.4740 | 828.4705 | 4.29 | 1 | 43 | 0.00049 | 1Score > 23 indicates identity |
U | R.EDIAILR.I | |
| 792 |
851.4180 | 850.4107 | 850.4086 | 2.52 | 0 | 15 | 0.18 | 1Score > 21 indicates identity Score > 20 indicates homology |
U | R.SWFQQR.L | |
| 793 |
426.2128 | 850.4110 | 850.4086 | 2.90 | 0 | 24 | 0.0073 | 1Score > 21 indicates identity Score > 15 indicates homology |
U | R.SWFQQR.L | |
| 854 |
438.2470 | 874.4794 | 874.4760 | 3.96 | 0 | 45 | 0.00019 | 1Score > 21 indicates identity |
U | K.TLQDLSAK.L | |
| 864 |
441.2886 | 880.5626 | 880.5607 | 2.26 | 0 | 23 | 0.0051 | 1Score > 13 indicates identity |
U | R.QVIRPLR.K | |
| 927 |
453.7532 | 905.4918 | 905.4858 | 6.64 | 0 | 11 | 0.14 | 1Score > 21 indicates identity Score > 15 indicates homology |
U | K.LLQDAFTV.- | |
| 1019 |
314.8510 | 941.5312 | 941.5294 | 1.88 | 1 | 27 | 0.011 | 1Score > 20 indicates identity |
U | K.SLVKEPNR.E | |
| 1022 |
471.7757 | 941.5368 | 941.5334 | 3.61 | 0 | 35 | 0.0019 | 1Score > 21 indicates identity |
U | R.DHFVLLAK.N | |
| 1041 |
949.5016 | 948.4943 | 948.4916 | 2.83 | 0 | 49 | 7.3e-005 | 1Score > 20 indicates identity |
U | R.DIFDALQK.T | |
| 1042 |
475.2563 | 948.4980 | 948.4916 | 6.76 | 0 | 4 | 1 | 1Score > 20 indicates identity Score > 17 indicates homology |
U | R.DIFDALQK.T | |
| 1129 |
1 |
496.2844 | 990.5542 | 990.5498 | 4.49 | 1 | 32 | 0.0026 | 1Score > 18 indicates identity |
U | K.ALVLEYAGR.D |
| 1134 |
497.8382 | 993.6618 | 993.6586 | 3.22 | 0 | 39 | 0.00012 | 1Score > 13 indicates identity |
U | R.KPLVVLTPK.S | |
| 1135 |
332.2280 | 993.6622 | 993.6586 | 3.55 | 0 | 81 | 7.9e-009 | 1Score > 13 indicates identity |
U | R.KPLVVLTPK.S | |
| 1236 |
521.7788 | 1041.5430 | 1041.5382 | 4.62 | 1 | 31 | 0.0038 | 1Score > 20 indicates identity |
U | K.VYYDLLEK.R | |
| 1318 |
542.7636 | 1083.5126 | 1083.5084 | 3.91 | 1 | 50 | 3.6e-005 | 1Score > 18 indicates identity |
U | R.STEYATDVAK.M | |
| 1358 |
1 |
552.8276 | 1103.6406 | 1103.6339 | 6.12 | 0 | 53 | 1.2e-005 | 1Score > 16 indicates identity |
U | R.LNVLVNTFGK.N |
| 1547 |
393.8888 | 1178.6446 | 1178.6408 | 3.23 | 0 | 34 | 0.0017 | 1Score > 18 indicates identity |
U | R.NALDNGLHVVK.S | |
| 1696 |
626.3293 | 1250.6440 | 1250.6329 | 8.90 | 0 | 67 | 6.2e-007 | 1Score > 20 indicates identity Score > 17 indicates homology |
U | R.DVVIDLVCYR.R | |
| 2690 |
576.6255 | 1726.8547 | 1726.8486 | 3.52 | 0 | 120 | 7e-012 | 1Score > 21 indicates identity |
U | K.LPADGSTATDVSHSTIR.D | |
| 3041 |
959.0056 | 1915.9966 | 1915.9792 | 9.11 | 0 | 59 | 7e-006 | 1Score > 20 indicates identity |
U | K.DASTYIPLQNLFPGQPR.F | |
| 3043 |
1 |
959.5050 | 1916.9954 | 1916.9632 | 16.8 | 0 | 50 | 4.6e-005 | 1Score > 19 indicates identity |
U | K.DASTYIPLQNLFPGQPR.F + Deamidated (NQ) |
| 3045 |
640.0077 | 1917.0013 | 1916.9632 | 19.9 | 0 | 39 | 0.00058 | 1Score > 19 indicates identity |
U | K.DASTYIPLQNLFPGQPR.F + Deamidated (NQ) | |
| 3948 |
841.7893 | 2522.3461 | 2522.3242 | 8.69 | 0 | 23 | 0.013 | 1Score > 16 indicates identity |
U | K.TGGTVHIVINNQVGFTISNPLDAR.S | |
| 3952 |
842.4532 | 2524.3378 | 2524.2922 | 18.1 | 0 | 9 | 0.36 | 1Score > 17 indicates identity |
U | K.TGGTVHIVINNQVGFTISNPLDAR.S + 2 Deamidated (NQ) | |
| 4667 |
1 |
903.4769 | 3609.8785 | 3609.8103 | 18.9 | 0 | 37 | 0.0003 | 1Score > 15 indicates identity |
U | K.LDPLGLWQRPAPVDLSINHYGLTNADLDTTFR.A + 2 Deamidated (NQ) |
13| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RPOC_PSEPK | 704 | DNA-directed RNA polymerase subunit beta' OS=Pseudomonas putida (strain KT2440) GN=rpoC PE=3 SV=1 |
14| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL3_PSEPK | 676 | 50S ribosomal protein L3 OS=Pseudomonas putida (strain KT2440) GN=rplC PE=3 SV=2 |
15| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88FB1_PSEPK | 674 | Dihydrolipoyl dehydrogenase OS=Pseudomonas putida (strain KT2440) GN=lpdG PE=4 SV=1 |
16| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RS2_PSEPK | 613 | 30S ribosomal protein S2 OS=Pseudomonas putida (strain KT2440) GN=rpsB PE=3 SV=1 |
17| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RS5_PSEPK | 611 | 30S ribosomal protein S5 OS=Pseudomonas putida (strain KT2440) GN=rpsE PE=3 SV=1 |
18| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88R99_PSEPK | 608 | Porin E OS=Pseudomonas putida (strain KT2440) GN=oprE PE=4 SV=1 |
19| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | CH60_PSEPK | 589 | 60 kDa chaperonin OS=Pseudomonas putida (strain KT2440) GN=groL PE=3 SV=1 |
20| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88PD7_PSEPK | 581 | Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_0913 PE=4 SV=1 |
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