MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita1 |
| MS data file | : | PRT1270_T-BRSC_1_20250714114344.mgf |
| Database | : | P-putida 20180924 (5,556 sequences; 1,892,173 residues) |
| Timestamp | : | 12 Aug 2025 at 22:47:09 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,938 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
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111| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL22_PSEPK | 128 | 50S ribosomal protein L22 OS=Pseudomonas putida (strain KT2440) GN=rplV PE=3 SV=1 |
112| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88NA3_PSEPK | 128 | Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_1309 PE=4 SV=1 |
113| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88N87_PSEPK | 128 | Lipoprotein, putative OS=Pseudomonas putida (strain KT2440) GN=PP_1325 PE=4 SV=1 |
114| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88FI0_PSEPK | 127 | Isocitrate lyase OS=Pseudomonas putida (strain KT2440) GN=aceA PE=4 SV=1 |
115| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88F46_PSEPK | 126 | Cbb3-type cytochrome c oxidase subunit OS=Pseudomonas putida (strain KT2440) GN=ccoP-1 PE=3 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 115.1 | Q88F46_PSEPK |
126 | 34122 | 5 (4) | 5 (4) | 0.30 | |
| Cbb3-type cytochrome c oxidase subunit OS=Pseudomonas putida (strain KT2440) GN=ccoP-1 PE=3 SV=1 | |||||||
| 1 sameset of Q88F46_PSEPK | |||||||
| Q88F41_PSEPK |
126 | 35819 | 4 (4) | 4 (4) | 0.28 | ||
| Cbb3-type cytochrome c oxidase subunit OS=Pseudomonas putida (strain KT2440) GN=ccoP-2 PE=3 SV=1 | |||||||
5 peptide matches (5 non-duplicate, 0 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 500 |
358.2035 | 714.3924 | 714.3912 | 1.76 | 1 | 17 | 0.045 | 1Score > 22 indicates identity Score > 16 indicates homology |
U | R.TLPEGAK.A | |
| 637 |
390.2277 | 778.4408 | 778.4378 | 3.97 | 0 | 34 | 0.0014 | 1Score > 18 indicates identity |
U | R.FGPIFAK.Y | |
| 1156 |
501.7493 | 1001.4840 | 1001.4818 | 2.23 | 1 | 3 | 3.8 | 3Score > 22 indicates identity |
U | R.WGGDPETIK.T | |
| 2410 |
805.3782 | 1608.7418 | 1608.7321 | 6.05 | 0 | 43 | 0.00012 | 1Score > 20 indicates identity Score > 16 indicates homology |
U | K.GAFGFPNLTDNDWR.W | |
| 4051 |
885.4629 | 2653.3669 | 2653.3210 | 17.3 | 1 | 85 | 1.4e-008 | 1Score > 19 indicates identity |
U | R.HGVMPAWAEVIGDQGVADVAAFVVSK.L + Deamidated (NQ) |
116| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88KX6_PSEPK | 126 | VacJ lipoprotein OS=Pseudomonas putida (strain KT2440) GN=vacJ PE=4 SV=1 |
117| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88HA4_PSEPK | 125 | Multidrug efflux RND transporter OS=Pseudomonas putida (strain KT2440) GN=PP_3456 PE=4 SV=1 |
118| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | KPRS_PSEPK | 125 | Ribose-phosphate pyrophosphokinase OS=Pseudomonas putida (strain KT2440) GN=prs PE=3 SV=1 |
119| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88L48_PSEPK | 124 | CmpX protein OS=Pseudomonas putida (strain KT2440) GN=cmpX PE=4 SV=1 |
120| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | DNAK_PSEPK | 119 | Chaperone protein DnaK OS=Pseudomonas putida (strain KT2440) GN=dnaK PE=3 SV=1 |
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