MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 12 Aug 2025 at 22:47:09 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 11–20 (out of 313)

Page: Previous 1 2 3 4 5 6 7 … 32 Next 

-11

Accession Score Description
Family member distances as a dendrogram 1 Q88NK1_PSEPK 752 Porin D OS=Pseudomonas putida (strain KT2440) GN=oprD PE=4 SV=1
3 Q88MB8_PSEPK 45 GTP pyrophosphokinase OS=Pseudomonas putida (strain KT2440) GN=relA PE=3 SV=1
2 Q88R66_PSEPK 273 Outer membrane protein OprE3 OS=Pseudomonas putida (strain KT2440) GN=oprQ PE=4 SV=1
Cut threshold

Score Mass Matches Sequences emPAI
Q88NK1_PSEPK 752 46092 40 (31) 21 (16) 2.21
Porin D OS=Pseudomonas putida (strain KT2440) GN=oprD PE=4 SV=1
Q88R66_PSEPK 273 47758 12 (10) 10 (8) 0.60
Outer membrane protein OprE3 OS=Pseudomonas putida (strain KT2440) GN=oprQ PE=4 SV=1
Q88MB8_PSEPK 45 84014 4 (2) 4 (2) 0.05
GTP pyrophosphokinase OS=Pseudomonas putida (strain KT2440) GN=relA PE=3 SV=1

-53 peptide matches (44 non-duplicate, 9 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U 1 2 3 Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U 1 2 3 Peptide
289   589.3196 588.3123 588.3119 0.74 0 43 0.00085 +1Score > 25 indicates identity U X R.DVDIK.Y
356   319.1694 636.3242 636.3231 1.77 0 26 0.022 +1Score > 22 indicates identity U X X DLSFR  
370   324.1667 646.3188 646.3187 0.24 0 28 0.018 +1Score > 24 indicates identity U X K.SWQAR.Y
371   647.3278 646.3205 646.3187 2.83 0 27 0.029 +1Score > 24 indicates identity U X K.SWQAR.Y
392   333.6772 665.3398 665.3384 2.12 0 19 0.034 +1Score > 20 indicates identity
Score > 17 indicates homology		 U X R.DSFGLK.E
393   666.3480 665.3407 665.3384 3.43 0 46 0.00014 +1Score > 20 indicates identity U X R.DSFGLK.E
614   387.7410 773.4674 773.4647 3.56 0 20 0.02 +1Score > 16 indicates identity U X R.ISNTVLK.Y
667   795.3905 794.3832 794.3810 2.77 1 46 0.00013 +1Score > 20 indicates identity U X K.GFIEDSK.L
668   398.1992 794.3838 794.3810 3.55 1 25 0.02 +1Score > 20 indicates identity U X K.GFIEDSK.L
710   408.7083 815.4020 815.4000 2.54 0 3 1.2 +5Score > 22 indicates identity
Score > 16 indicates homology		 U X R.MLYFSR.D
833   434.2046 866.3946 866.3923 2.76 1 21 0.032 +1Score > 19 indicates identity U X K.VEDYWR.K
896   894.4710 893.4637 893.4719 -9.14 1 4 2.4 +8Score > 20 indicates identity U X R.VRQATYR.S + Deamidated (NQ)
951   460.2550 918.4954 918.4923 3.43 1 42 0.00033 +1Score > 20 indicates identity
Score > 20 indicates homology		 U X R.EIFNQIR.Y
1107   488.7600 975.5054 975.5025 2.97 0 36 0.002 +1Score > 22 indicates identity U X R.YVVQDGPAK.D
1159   502.2817 1002.5488 1002.5346 14.3 1 13 0.3 +2Score > 20 indicates identity U X K.VTLAEVSQR.F + Deamidated (NQ)
1160   1004.5444 1003.5371 1003.5338 3.28 1 63 3.9e-006 +1Score > 21 indicates identity U X K.YVLQEGPAK.D
1161   502.7768 1003.5390 1003.5338 5.20 1 39 0.00094 +1Score > 21 indicates identity U X K.YVLQEGPAK.D
1231   519.2873 1036.5600 1036.5553 4.58 0 38 0.00066 +1Score > 18 indicates identity U X K.SINVLGGSYK.F
1269   354.2078 1059.6016 1059.5825 18.0 0 3 1.9 +2Score > 18 indicates identity U X R.AYDRPGLLR.D
1467   384.1887 1149.5443 1149.5414 2.46 1 43 0.00028 +1Score > 21 indicates identity
Score > 20 indicates homology		 U X R.AFDGDKLDNR.A
1468   575.7801 1149.5456 1149.5414 3.66 1 52 5.2e-005 +1Score > 21 indicates identity U X R.AFDGDKLDNR.A
1501 +3 580.3580 1158.7014 1158.6900 9.89 1 39 0.00013 +1Score > 13 indicates identity U X R.LIVEYPLSIL.-
1502 +2 1159.7113 1158.7040 1158.6900 12.1 1 51 7.5e-006 +1Score > 13 indicates identity U X R.LIVEYPLSIL.-
1613   607.3328 1212.6510 1212.6431 6.58 1 1 1 +1Score > 21 indicates identity
Score > 14 indicates homology		 U X R.VFVEYPISIF.-
1669 +1 618.3076 1234.6006 1234.5942 5.19 0 46 0.00025 +1Score > 22 indicates identity U X R.AGTGLFPTGSDGR.S
1843   667.8602 1333.7058 1333.6990 5.13 0 52 3.5e-005 +1Score > 20 indicates identity U X K.LAYVVGDNINTR.N
2112   490.9308 1469.7706 1469.7627 5.38 1 60 5.3e-006 +1Score > 20 indicates identity U X K.GFIEDSHANVLLR.N
2210   753.9332 1505.8518 1505.8341 11.8 1 66 4.4e-007 +1Score > 15 indicates identity U X R.LLPESFTGTLITSK.E
2844 +1 901.9124 1801.8102 1801.8006 5.34 1 90 4.5e-009 +1Score > 19 indicates identity U X R.SSDGVYYDSPSIDELR.L
2910 +1 919.9548 1837.8950 1837.8847 5.64 0 92 2.4e-009 +1Score > 21 indicates identity
Score > 18 indicates homology		 U X K.VGDQFTALPVFATDDSR.L
2914   613.9751 1838.9035 1838.8687 18.9 0 63 3.8e-006 +1Score > 21 indicates identity U X K.VGDQFTALPVFATDDSR.L + Deamidated (NQ)
3347   703.6845 2108.0317 2107.9925 18.6 1 8 0.93 +1Score > 20 indicates identity U X R.YDLNFAEYGIPGLTFMTR.Y + Deamidated (NQ)
3632   754.3933 2260.1581 2260.1164 18.4 0 17 0.084 +1Score > 19 indicates identity U X R.AFSLSGAYNIGAHTFTLAYQK.V + Deamidated (NQ)
3895 +1 826.0708 2475.1906 2475.1554 14.2 0 45 0.00013 +1Score > 19 indicates identity U X K.VTGDGDYGYGIDGGGTIFLANSVAR.S + Deamidated (NQ)
3926   834.0709 2499.1909 2499.1805 4.13 1 16 0.1 +1Score > 19 indicates identity U X K.EANVVGGTYAFTDNLSTSLYYSK.V
3927   834.4097 2500.2073 2500.1646 17.1 1 16 0.13 +1Score > 20 indicates identity U X K.EANVVGGTYAFTDNLSTSLYYSK.V + Deamidated (NQ)
4237   959.4918 2875.4536 2875.4068 16.3 0 63 1.4e-006 +1Score > 17 indicates identity U X K.HYLGLNYVFPIASDQSLTLDFNGYK.S + Deamidated (NQ)
4464   1061.5239 3181.5499 3181.4920 18.2 0 42 0.00016 +1Score > 17 indicates identity U X K.YYANVNWALPISDNQGLVFDFNIYDTK.S + 2 Deamidated (NQ)
4465   1061.5240 3181.5502 3181.4920 18.3 0 9 0.34 +1Score > 17 indicates identity U X K.YYANVNWALPISDNQGLVFDFNIYDTK.S + 2 Deamidated (NQ)
4477   797.6564 3186.5965 3186.5582 12.0 1 39 0.00042 +1Score > 18 indicates identity U X R.VHQPVNTDGSINLEAWLDHVVSVDSALDR.A + Deamidated (NQ)
4539   817.4077 3265.6017 3265.5428 18.0 0 12 0.19 +1Score > 18 indicates identity U X K.AAGLNGDSNTIWSLAATYAYGAHSFTLAHQR.S + 2 Deamidated (NQ)
4559   1103.8885 3308.6437 3308.6030 12.3 1 68 5.2e-007 +1Score > 17 indicates identity U X R.KYYANVNWALPISDNQGLVFDFNIYDTK.S + Deamidated (NQ)

3 subsets and intersections (4 subset proteins in total)

Score Mass Subset of
Q88RT3_PSEPK 81 0 11.1
description
Q88MZ9_PSEPK 31 0 11.1
description
Q88EK9_PSEPK 26 0 11.1
description
1 sameset of Q88EK9_PSEPK
Q88NY6_PSEPK 26 70584
Histidine kinase OS=Pseudomonas putida (strain KT2440) GN=PP_1067 PE=4 SV=1

+12

Accession Score Description
1 Q88FA9_PSEPK 748 2-oxoglutarate dehydrogenase, E1 component OS=Pseudomonas putida (strain KT2440) GN=kgdA PE=4 SV=1

+13

Accession Score Description
1 RPOC_PSEPK 704 DNA-directed RNA polymerase subunit beta' OS=Pseudomonas putida (strain KT2440) GN=rpoC PE=3 SV=1

+14

Accession Score Description
1 RL3_PSEPK 676 50S ribosomal protein L3 OS=Pseudomonas putida (strain KT2440) GN=rplC PE=3 SV=2

+15

Accession Score Description
1 Q88FB1_PSEPK 674 Dihydrolipoyl dehydrogenase OS=Pseudomonas putida (strain KT2440) GN=lpdG PE=4 SV=1

+16

Accession Score Description
1 RS2_PSEPK 613 30S ribosomal protein S2 OS=Pseudomonas putida (strain KT2440) GN=rpsB PE=3 SV=1

+17

Accession Score Description
1 RS5_PSEPK 611 30S ribosomal protein S5 OS=Pseudomonas putida (strain KT2440) GN=rpsE PE=3 SV=1

+18

Accession Score Description
1 Q88R99_PSEPK 608 Porin E OS=Pseudomonas putida (strain KT2440) GN=oprE PE=4 SV=1

+19

Accession Score Description
1 CH60_PSEPK 589 60 kDa chaperonin OS=Pseudomonas putida (strain KT2440) GN=groL PE=3 SV=1

+20

Accession Score Description
1 Q88PD7_PSEPK 581 Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_0913 PE=4 SV=1
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