MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita1 |
| MS data file | : | PRT1270_T-BRSC_1_20250714114344.mgf |
| Database | : | P-putida 20180924 (5,556 sequences; 1,892,173 residues) |
| Timestamp | : | 12 Aug 2025 at 22:47:09 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,938 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
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101| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88NV5_PSEPK | 136 | Cold-shock domain family protein OS=Pseudomonas putida (strain KT2440) GN=PP_1099 PE=4 SV=1 |
102| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88N90_PSEPK | 135 | Lipoprotein, putative OS=Pseudomonas putida (strain KT2440) GN=PP_1322 PE=4 SV=1 |
103| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL25_PSEPK | 134 | 50S ribosomal protein L25 OS=Pseudomonas putida (strain KT2440) GN=rplY PE=3 SV=2 |
104| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | TIG_PSEPK | 133 | Trigger factor OS=Pseudomonas putida (strain KT2440) GN=tig PE=3 SV=2 |
105| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88CK1_PSEPK | 131 | Polyamine-transporting ATPase OS=Pseudomonas putida (strain KT2440) GN=potG PE=3 SV=1 |
106| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88CA3_PSEPK | 131 | Aldehyde dehydrogenase family protein OS=Pseudomonas putida (strain KT2440) GN=PP_5278 PE=3 SV=1 |
107| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88PL5_PSEPK | 130 | Protein translocase subunit SecD OS=Pseudomonas putida (strain KT2440) GN=secD PE=3 SV=1 |
108| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | METN1_PSEPK | 129 | Methionine import ATP-binding protein MetN 1 OS=Pseudomonas putida (strain KT2440) GN=metN1 PE=3 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 108.1 | METN1_PSEPK |
129 | 36761 | 9 (2) | 8 (2) | 0.13 | |
| Methionine import ATP-binding protein MetN 1 OS=Pseudomonas putida (strain KT2440) GN=metN1 PE=3 SV=1 | |||||||
9 peptide matches (9 non-duplicate, 0 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 226 |
515.3321 | 514.3248 | 514.3227 | 4.08 | 0 | 5 | 4.1 | 8Score > 26 indicates identity Score > 23 indicates homology |
R.VGIAR.A | ||
| 246 |
535.3007 | 534.2934 | 534.2914 | 3.77 | 0 | 6 | 0.85 | 2Score > 18 indicates identity |
E.AAFAR.F | ||
| 535 |
366.2067 | 730.3988 | 730.3861 | 17.5 | 0 | 7 | 2.8 | 9Score > 24 indicates identity |
U | R.ALSTNPK.I + Deamidated (NQ) | |
| 717 |
822.4489 | 821.4416 | 821.4508 | -11.1 | 1 | 8 | 0.69 | 6Score > 19 indicates identity |
U | K.TYRVAGR.E | |
| 1426 |
566.2876 | 1130.5606 | 1130.5642 | -3.11 | 0 | 1 | 8.3 | 8Score > 22 indicates identity |
U | R.VAVMDAGQIVE.Q | |
| 2359 |
791.4130 | 1580.8114 | 1580.8059 | 3.48 | 1 | 14 | 0.25 | 2Score > 21 indicates identity |
U | E.DVTAFNASQLRGFR.Q | |
| 2360 |
527.9451 | 1580.8135 | 1580.8059 | 4.77 | 1 | 5 | 1.9 | 2Score > 21 indicates identity Score > 20 indicates homology |
U | E.DVTAFNASQLRGFR.Q | |
| 2867 |
906.0152 | 1810.0158 | 1809.9836 | 17.8 | 1 | 78 | 3.4e-008 | 1Score > 16 indicates identity |
U | K.TVADNVALPLALAGELSR.S + Deamidated (NQ) | |
| 3009 |
948.0157 | 1894.0168 | 1893.9836 | 17.5 | 0 | 68 | 5.7e-007 | 1Score > 18 indicates identity |
U | R.LTFQGDATYAPLLGTVAR.E + Deamidated (NQ) |
109| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88C77_PSEPK | 129 | Ferric siderophore transport system protein ExbB OS=Pseudomonas putida (strain KT2440) GN=exbB PE=3 SV=1 |
110| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | EFTS_PSEPK | 128 | Elongation factor Ts OS=Pseudomonas putida (strain KT2440) GN=tsf PE=3 SV=1 |
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