MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita1 |
| MS data file | : | PRT1270_T-BRSC_1_20250714114344.mgf |
| Database | : | P-putida 20180924 (5,556 sequences; 1,892,173 residues) |
| Timestamp | : | 12 Aug 2025 at 22:47:09 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,938 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
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101| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88NV5_PSEPK | 136 | Cold-shock domain family protein OS=Pseudomonas putida (strain KT2440) GN=PP_1099 PE=4 SV=1 |
102| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88N90_PSEPK | 135 | Lipoprotein, putative OS=Pseudomonas putida (strain KT2440) GN=PP_1322 PE=4 SV=1 |
103| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL25_PSEPK | 134 | 50S ribosomal protein L25 OS=Pseudomonas putida (strain KT2440) GN=rplY PE=3 SV=2 |
104| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | TIG_PSEPK | 133 | Trigger factor OS=Pseudomonas putida (strain KT2440) GN=tig PE=3 SV=2 |
105| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88CK1_PSEPK | 131 | Polyamine-transporting ATPase OS=Pseudomonas putida (strain KT2440) GN=potG PE=3 SV=1 |
106| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88CA3_PSEPK | 131 | Aldehyde dehydrogenase family protein OS=Pseudomonas putida (strain KT2440) GN=PP_5278 PE=3 SV=1 |
107| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88PL5_PSEPK | 130 | Protein translocase subunit SecD OS=Pseudomonas putida (strain KT2440) GN=secD PE=3 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 107.1 | Q88PL5_PSEPK |
130 | 66929 | 9 (5) | 9 (5) | 0.18 | |
| Protein translocase subunit SecD OS=Pseudomonas putida (strain KT2440) GN=secD PE=3 SV=1 | |||||||
9 peptide matches (9 non-duplicate, 0 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 322 |
308.6876 | 615.3606 | 615.3592 | 2.42 | 0 | 15 | 0.44 | 4Score > 24 indicates identity |
U | K.ALVDAK.I | |
| 404 |
671.3745 | 670.3672 | 670.3650 | 3.35 | 0 | 18 | 0.051 | 4Score > 18 indicates identity |
U | E.DQLPAK.D | |
| 564 |
372.7351 | 743.4556 | 743.4541 | 2.06 | 0 | 4 | 1.9 | 9Score > 19 indicates identity |
U | R.LALTQAK.V | |
| 707 |
408.2351 | 814.4556 | 814.4548 | 1.00 | 1 | 3 | 6.7 | 9Score > 23 indicates identity |
U | R.NELAVLR.L + Deamidated (NQ) | |
| 790 |
425.7255 | 849.4364 | 849.4344 | 2.35 | 1 | 18 | 0.082 | 1Score > 22 indicates identity Score > 19 indicates homology |
U | K.TANLEFR.F | |
| 2033 |
712.4032 | 1422.7918 | 1422.7831 | 6.17 | 1 | 37 | 0.00052 | 1Score > 17 indicates identity |
U | R.VNELGVAEPLVQR.Q | |
| 2698 |
866.0013 | 1729.9880 | 1729.9727 | 8.90 | 0 | 35 | 0.00035 | 1Score > 13 indicates identity |
U | K.IISLATIQSPLGSQFR.I | |
| 3765 |
782.0862 | 2343.2368 | 2343.2223 | 6.18 | 0 | 71 | 2.7e-007 | 1Score > 18 indicates identity |
U | K.ALGDDYVVALNLAQTTPQWLR.N | |
| 4284 |
735.3846 | 2937.5093 | 2937.4581 | 17.4 | 1 | 34 | 0.0011 | 1Score > 17 indicates identity |
U | R.GLIITGDQVTDAQASFDEHGRPQVNIR.L + Deamidated (NQ) |
| Score | Mass | Subset of | |||
|---|---|---|---|---|---|
| Q88FF1_PSEPK |
18 | 69669 | 107.1 | ||
| Peptide ABC transporter, periplamic peptide-binding protein OS=Pseudomonas putida (strain KT2440) GN=PP_4146 PE=4 SV=1 | |||||
108| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | METN1_PSEPK | 129 | Methionine import ATP-binding protein MetN 1 OS=Pseudomonas putida (strain KT2440) GN=metN1 PE=3 SV=1 |
109| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88C77_PSEPK | 129 | Ferric siderophore transport system protein ExbB OS=Pseudomonas putida (strain KT2440) GN=exbB PE=3 SV=1 |
110| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | EFTS_PSEPK | 128 | Elongation factor Ts OS=Pseudomonas putida (strain KT2440) GN=tsf PE=3 SV=1 |
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