MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita1 |
| MS data file | : | PRT1270_T-BRSC_1_20250714114344.mgf |
| Database | : | P-putida 20180924 (5,556 sequences; 1,892,173 residues) |
| Timestamp | : | 12 Aug 2025 at 22:47:09 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,938 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle () in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
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101| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88NV5_PSEPK | 136 | Cold-shock domain family protein OS=Pseudomonas putida (strain KT2440) GN=PP_1099 PE=4 SV=1 |
102| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88N90_PSEPK | 135 | Lipoprotein, putative OS=Pseudomonas putida (strain KT2440) GN=PP_1322 PE=4 SV=1 |
103| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL25_PSEPK | 134 | 50S ribosomal protein L25 OS=Pseudomonas putida (strain KT2440) GN=rplY PE=3 SV=2 |
104| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | TIG_PSEPK | 133 | Trigger factor OS=Pseudomonas putida (strain KT2440) GN=tig PE=3 SV=2 |
105| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88CK1_PSEPK | 131 | Polyamine-transporting ATPase OS=Pseudomonas putida (strain KT2440) GN=potG PE=3 SV=1 |
106| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88CA3_PSEPK | 131 | Aldehyde dehydrogenase family protein OS=Pseudomonas putida (strain KT2440) GN=PP_5278 PE=3 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 106.1 | Q88CA3_PSEPK |
131 | 53249 | 4 (4) | 4 (4) | 0.18 | |
| Aldehyde dehydrogenase family protein OS=Pseudomonas putida (strain KT2440) GN=PP_5278 PE=3 SV=1 | |||||||
4 peptide matches (4 non-duplicate, 0 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 543 |
367.7147 | 733.4148 | 733.4123 | 3.53 | 0 | 25 | 0.015 | 1Score > 19 indicates identity |
U | R.FADLLR.K | |
| 2808 |
594.6807 | 1781.0203 | 1780.9934 | 15.1 | 1 | 17 | 0.018 | 1Score > 13 indicates identity |
U | K.LGPALATGNSVVLKPSEK.S + Deamidated (NQ) | |
| 4053 |
887.1788 | 2658.5146 | 2658.4745 | 15.1 | 1 | 62 | 6e-007 | 1Score > 13 indicates identity |
U | R.IAQLAIEAGIPAGVLNVLPGYGHTVGK.A + Deamidated (NQ) | |
| 4695 |
942.4879 | 3765.9225 | 3765.8672 | 14.7 | 1 | 70 | 1.3e-007 | 1Score > 14 indicates identity |
U | K.GWKPGNPLDPQTTVGALVDTQQMNTVLSYIEAGHK.D + Deamidated (NQ) |
107| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88PL5_PSEPK | 130 | Protein translocase subunit SecD OS=Pseudomonas putida (strain KT2440) GN=secD PE=3 SV=1 |
108| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | METN1_PSEPK | 129 | Methionine import ATP-binding protein MetN 1 OS=Pseudomonas putida (strain KT2440) GN=metN1 PE=3 SV=1 |
109| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88C77_PSEPK | 129 | Ferric siderophore transport system protein ExbB OS=Pseudomonas putida (strain KT2440) GN=exbB PE=3 SV=1 |
110| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | EFTS_PSEPK | 128 | Elongation factor Ts OS=Pseudomonas putida (strain KT2440) GN=tsf PE=3 SV=1 |
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