MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 12 Aug 2025 at 22:47:09 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 1–10 (out of 313)

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-1

Accession Score Description
1 Q88NM2_PSEPK 4307 Outer membrane protein H1 OS=Pseudomonas putida (strain KT2440) GN=oprH PE=4 SV=1
Score Mass Matches Sequences emPAI
1.1 Q88NM2_PSEPK 4307 21545 103 (92) 15 (15) 26.00
Outer membrane protein H1 OS=Pseudomonas putida (strain KT2440) GN=oprH PE=4 SV=1

-103 peptide matches (37 non-duplicate, 66 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
417 +1 673.4011 672.3938 672.3919 2.92 0 18 0.19 +3Score > 23 indicates identity U K.SGLLQR.N
418 +2 337.2051 672.3956 672.3919 5.63 0 47 0.00014 +1Score > 21 indicates identity U K.SGLLQR.N
689 +3 401.2518 800.4890 800.4868 2.79 1 34 0.0021 +1Score > 20 indicates identity U R.KSGLLQR.N
691   801.4975 800.4902 800.4868 4.26 1 31 0.0041 +1Score > 20 indicates identity U R.KSGLLQR.N
778 +1 848.3915 847.3842 847.3824 2.10 0 34 0.0029 +1Score > 21 indicates identity U K.DDTWGVR.L
782 +2 424.6999 847.3852 847.3824 3.31 0 36 0.00086 +1Score > 21 indicates identity
Score > 18 indicates homology		 U K.DDTWGVR.L
D:\Xcalibur\Data\Jennifer\PRT1270 Rita DDA\PRT1270_T-BRSC_1_20250714114344.raw

Score > 20 indicates identity
891 +2 894.4703 893.4630 893.4607 2.64 1 41 0.00052 -1Score > 20 indicates identity U K.ASVELGYR.Y
-9.91 1 10 0.57 2 RTLANYR   + Deamidated (NQ)
2.63 1 10 0.61 3 DIFDKTR  
-18.2 0 9 0.8 4 NVFMVIR   + Oxidation (M)
-14.5 0 7 1.3 5 WSSFVLR  
9.95 1 6 1.7 6 AMRTPYR  
2.63 0 5 1.8 7 GSLFDSLR  
-9.94 1 3 3.1 8 TRIFDSR  
-1.13 0 1 4.6 9 LASSITMR   + Oxidation (M)
892 +3 447.7388 893.4630 893.4607 2.67 1 47 0.00012 +1Score > 20 indicates identity U K.ASVELGYR.Y
1208   1031.5146 1030.5073 1030.5043 2.90 0 58 1.5e-005 +1Score > 22 indicates identity U K.LTQDSPGASR.D
1209 +2 516.2616 1030.5086 1030.5043 4.19 0 68 1.5e-006 +1Score > 22 indicates identity U K.LTQDSPGASR.D
1222 +3 518.2962 1034.5778 1034.5761 1.72 0 69 3.4e-007 +1Score > 17 indicates identity U K.LFGGGSLGVTK.L
1227 +2 1035.5902 1034.5829 1034.5761 6.63 0 91 2.3e-009 +1Score > 17 indicates identity U K.LFGGGSLGVTK.L
1799 +1 655.3304 1308.6462 1308.6422 3.07 1 77 1.4e-007 +1Score > 21 indicates identity U R.TNAATEVGAHGGPK.D
1801 +2 437.2231 1308.6475 1308.6422 4.01 1 66 1.4e-006 +1Score > 21 indicates identity
Score > 20 indicates homology		 U R.TNAATEVGAHGGPK.D
1911 +2 456.5702 1366.6888 1366.6841 3.43 0 60 3.4e-006 +1Score > 20 indicates identity
Score > 18 indicates homology		 U R.NTDHLNADGIIGK.D
1913 +2 684.3520 1366.6894 1366.6841 3.93 0 71 4.2e-007 +1Score > 20 indicates identity U R.NTDHLNADGIIGK.D
2189 +14 752.3901 1502.7656 1502.7616 2.67 0 103 3.6e-010 +1Score > 21 indicates identity U R.LTSSAQTYLAASYK.F
2192   501.9298 1502.7676 1502.7616 3.95 0 27 0.0088 +1Score > 21 indicates identity
Score > 19 indicates homology		 U R.LTSSAQTYLAASYK.F
2206   502.2605 1503.7597 1503.7457 9.32 0 36 0.00068 +1Score > 21 indicates identity
Score > 16 indicates homology		 U R.LTSSAQTYLAASYK.F + Deamidated (NQ)
2511   550.9509 1649.8309 1649.8301 0.49 1 26 0.015 +1Score > 20 indicates identity U R.LTSSAQTYLAASYKF.-
2513 +9 825.9270 1649.8394 1649.8301 5.69 1 120 5.4e-012 +1Score > 20 indicates identity U R.LTSSAQTYLAASYKF.-
2929   925.4063 1848.7980 1848.7989 -0.44 0 101 1.5e-010 +1Score > 15 indicates identity U R.YYMTYDNVSGDHSGLK.L
2930 +1 617.2750 1848.8032 1848.7989 2.33 0 70 2.4e-007 +1Score > 16 indicates identity U R.YYMTYDNVSGDHSGLK.L
3349 +1 1055.5210 2109.0274 2109.0630 -16.9 1 18 0.088 +1Score > 20 indicates identity U R.QENLLGSYDLFLPVGDTTK.L
3354 +3 1056.0457 2110.0768 2110.0470 14.1 1 88 6.9e-009 +1Score > 19 indicates identity U R.QENLLGSYDLFLPVGDTTK.L + Deamidated (NQ)
3356 +2 704.3669 2110.0789 2110.0470 15.1 1 32 0.0026 +1Score > 19 indicates identity U R.QENLLGSYDLFLPVGDTTK.L + Deamidated (NQ)
3535   1099.5398 2197.0650 2197.0400 11.4 1 102 3.1e-010 +1Score > 20 indicates identity U R.NTDHLNADGIIGKDDTWGVR.L + Deamidated (NQ)
3537 +1 733.3645 2197.0717 2197.0400 14.4 1 83 2.5e-008 +1Score > 20 indicates identity U R.NTDHLNADGIIGKDDTWGVR.L + Deamidated (NQ)
3538 +1 550.2752 2197.0717 2197.0400 14.4 1 52 1.8e-005 +1Score > 20 indicates identity
Score > 17 indicates homology		 U R.NTDHLNADGIIGKDDTWGVR.L + Deamidated (NQ)
3539   550.2756 2197.0733 2197.0400 15.2 1 45 6.4e-005 +1Score > 20 indicates identity
Score > 15 indicates homology		 U R.NTDHLNADGIIGKDDTWGVR.L + Deamidated (NQ)
3541   733.3655 2197.0747 2197.0400 15.8 1 60 5.2e-006 +1Score > 20 indicates identity U R.NTDHLNADGIIGKDDTWGVR.L + Deamidated (NQ)
3707   769.3698 2305.0876 2305.0750 5.44 0 33 0.0022 +1Score > 19 indicates identity U R.DTDYGYAYGLQAGVIQDITDK.A
3708 +2 1153.5514 2305.0882 2305.0750 5.74 0 118 6.6e-012 +1Score > 19 indicates identity U R.DTDYGYAYGLQAGVIQDITDK.A
3712 +3 1154.0543 2306.0940 2306.0590 15.2 0 119 5.4e-012 +1Score > 19 indicates identity U R.DTDYGYAYGLQAGVIQDITDK.A + Deamidated (NQ)
3714   769.7081 2306.1025 2306.0590 18.8 0 64 1.8e-006 +1Score > 20 indicates identity U R.DTDYGYAYGLQAGVIQDITDK.A + Deamidated (NQ)
3715 +1 769.7082 2306.1028 2306.0590 19.0 0 74 2.1e-007 +1Score > 19 indicates identity U R.DTDYGYAYGLQAGVIQDITDK.A + Deamidated (NQ)
4564   1107.2048 3318.5926 3318.5528 12.0 1 57 5.9e-006 +1Score > 17 indicates identity U K.LTQDSPGASRDTDYGYAYGLQAGVIQDITDK.A + Deamidated (NQ)

+2

Accession Score Description
Family member distances as a dendrogram 1 EFTU2_PSEPK 2609 Elongation factor Tu-B OS=Pseudomonas putida (strain KT2440) GN=tufB PE=3 SV=1
2 EFTU1_PSEPK 2412 Elongation factor Tu-A OS=Pseudomonas putida (strain KT2440) GN=tufA PE=1 SV=1

+3

Accession Score Description
1 ATPB_PSEPK 2344 ATP synthase subunit beta OS=Pseudomonas putida (strain KT2440) GN=atpD PE=3 SV=1

+4

Accession Score Description
1 Q88ES5_PSEPK 2322 Flagellin OS=Pseudomonas putida (strain KT2440) GN=fliC PE=3 SV=1

+5

Accession Score Description
1 Q88L46_PSEPK 1745 Outer membrane protein OprF OS=Pseudomonas putida (strain KT2440) GN=oprF PE=3 SV=1

+6

Accession Score Description
1 ATPA_PSEPK 1711 ATP synthase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=atpA PE=3 SV=1

+7

Accession Score Description
1 Q88FB0_PSEPK 1295 Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex OS=Pseudomonas putida (strain KT2440) GN=kgdB PE=3 SV=1

+8

Accession Score Description
1 Q88FA7_PSEPK 1233 Succinate dehydrogenase flavoprotein subunit OS=Pseudomonas putida (strain KT2440) GN=sdhA PE=3 SV=1

+9

Accession Score Description
1 RPOB_PSEPK 843 DNA-directed RNA polymerase subunit beta OS=Pseudomonas putida (strain KT2440) GN=rpoB PE=3 SV=1

+10

Accession Score Description
1 Q88FA8_PSEPK 775 Succinate dehydrogenase, iron-sulfur protein OS=Pseudomonas putida (strain KT2440) GN=sdhB PE=4 SV=1
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